Live-cell imaging of rhabdovirus-induced morphological changes in plant nuclear membranes.
AuthorsGoodin M, Yelton S, Ghosh D, Mathews S, Lesnaw J
JournalMol Plant Microbe Interact
PubMed ID16042016
'Potato yellow dwarf virus (PYDV) and Sonchus yellow net virus (SYNV) belong to the genus Nucleorhabdovirus. These viruses replicate in nuclei of infected cells and mature virions accumulate in the perinuclear space after budding through the inner nuclear membrane. Infection of transgenic Nicotiana benthamiana 16c plants (which constitutively express green ... More
Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.
AuthorsKim M, Bellini M, Ceman S,
JournalMol Cell Biol
PubMed ID18936162
The fragile X mental retardation protein FMRP is an RNA binding protein that associates with a large collection of mRNAs. Since FMRP was previously shown to be a nucleocytoplasmic shuttling protein, we examined the hypothesis that FMRP binds its cargo mRNAs in the nucleus. The enhanced green fluorescent protein-tagged FMRP ... More
Transcriptional repression mediated by repositioning of genes to the nuclear lamina.
AuthorsReddy KL, Zullo JM, Bertolino E, Singh H,
JournalNature
PubMed ID18272965
Nuclear compartmentalization seems to have an important role in regulating metazoan genes. Although studies on immunoglobulin and other loci have shown a correlation between positioning at the nuclear lamina and gene repression, the functional consequences of this compartmentalization remain untested. We devised an approach for inducible tethering of genes to ... More
Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli.
AuthorsBendezú FO, de Boer PA,
JournalJ Bacteriol
PubMed ID17993535
Maintenance of rod shape in Escherichia coli requires the shape proteins MreB, MreC, MreD, MrdA (PBP2), and MrdB (RodA). How loss of the Mre proteins affects E. coli viability has been unclear. We generated Mre and Mrd depletion strains under conditions that minimize selective pressure for undefined suppressors and found ... More
POLKADOTS are foci of functional interactions in T-Cell receptor-mediated signaling to NF-kappaB.
Stimulation of the T-cell receptor (TCR) results in the activation of several transcription factors, including NF-kappaB, that are crucial for T-cell proliferation and gain of effector functions. On TCR engagement, several proteins within the TCR-directed NF-kappaB signaling pathway undergo dynamic spatial redistribution, but the significance of these redistribution events is ... More
GLUT1 deficiency links nutrient availability and apoptosis during embryonic development.
AuthorsJensen PJ, Gitlin JD, Carayannopoulos MO
JournalJ Biol Chem
PubMed ID16543226
GLUT1 is essential for human brain development and function, as evidenced by the severe epileptic encephalopathy observed in children with GLUT1 deficiency syndrome resulting from inherited loss-of-function mutations in the gene encoding this facilitative glucose transporter. To further elucidate the pathophysiology of this disorder, the zebrafish orthologue of human GLUT1 ... More
Visualizing morphogenesis in transgenic zebrafish embryos using BODIPY TR methyl ester dye as a vital counterstain for GFP.
AuthorsCooper MS, Szeto DP, Sommers-Herivel G, Topczewski J, Solnica-Krezel L, Kang HC, Johnson I, Kimelman D
JournalDev Dyn
PubMed ID15614774
Green fluorescent protein (GFP) technology is rapidly advancing the study of morphogenesis, by allowing researchers to specifically focus on a subset of labeled cells within the living embryo. However, when imaging GFP-labeled cells using confocal microscopy, it is often essential to simultaneously visualize all of the cells in the embryo ... More
Targeting of amacrine cell neurites to appropriate synaptic laminae in the developing zebrafish retina.
AuthorsGodinho L, Mumm JS, Williams PR, Schroeter EH, Koerber A, Park SW, Leach SD, Wong RO
JournalDevelopment
PubMed ID16258076
Cellular mechanisms underlying the precision by which neurons target their synaptic partners have largely been determined based on the study of projection neurons. By contrast, little is known about how interneurons establish their local connections in vivo. Here, we investigated how developing amacrine interneurons selectively innervate the appropriate region of ... More
Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.
Authors
JournalNat Commun
PubMed ID31980602
Zebrafish Retinal Ganglion Cells Asymmetrically Encode Spectral and Temporal Information across Visual Space.
Authors
JournalCurr Biol
PubMed ID32531283
Nitric Oxide Produced by Macrophages Inhibits Adipocyte Differentiation and Promotes Profibrogenic Responses in Preadipocytes to Induce Adipose Tissue Fibrosis.
Authors
JournalDiabetes
PubMed ID27246913
High-density three-dimensional localization microscopy across large volumes.
Authors
JournalNat Methods
PubMed ID26950745
Fovea-like Photoreceptor Specializations Underlie Single UV Cone Driven Prey-Capture Behavior in Zebrafish.
Authors
JournalNeuron
PubMed ID32473094
eIF4EBP3L acts as a gatekeeper of TORC1 in activity-dependent muscle growth by specifically regulating Mef2ca translational initiation.
Authors
JournalPLoS Biol
PubMed ID24143132
Disturbed retinoid metabolism upon loss of rlbp1a impairs cone function and leads to subretinal lipid deposits and photoreceptor degeneration in the zebrafish retina.
Authors
JournalElife
PubMed ID34668483
Autocrine vitamin D signaling switches off pro-inflammatory programs of TH1 cells.
Authors
JournalNat Immunol
PubMed ID34764490
Strip1 regulates retinal ganglion cell survival by suppressing Jun-mediated apoptosis to promote retinal neural circuit formation.
Authors
JournalElife
PubMed ID35314028
In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation.