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Citas y referencias (48)
Invitrogen™
Carboxy-DCFDA (5-(and-6)-Carboxy-2',7'-Dichlorofluorescein Diacetate), mixed isomers
El carboxi-DCFDA puede difundirse pasivamente en las células y es incoloro y no fluorescente hasta que los grupos de acetatoMás información
| Número de catálogo | Cantidad |
|---|---|
| C369 | 100 mg |
Número de catálogo C369
Precio (MXN)
-
Cantidad:
100 mg
El carboxi-DCFDA puede difundirse pasivamente en las células y es incoloro y no fluorescente hasta que los grupos de acetato son escindidos por las esterasas intracelulares para producir el fluoróforo fluorescente, 5-(y-6)-carboxi-2',7' -diclorofluoresceína. Con una pKa de 4,8, este colorante puede usarse como un sensor de pH ácido.
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteIndicador de pH
Peso molecular529.29
Cantidad100 mg
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Trazador polar, Indicador de pH
Para utilizar con (equipo)Microscopio de fluorescencia
Tipo de productoCarboxi-DCFDA
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador entre -5 °C y -30 °C
Citations & References (48)
Citations & References
Abstract
Mitochondrial production of reactive oxygen species mediate dicumarol-induced cytotoxicity in cancer cells.
Journal:J Biol Chem
PubMed ID:17040906
'Dicumarol is a naturally occurring anticoagulant derived from coumarin that induces cytotoxicity and oxidative stress in human pancreatic cancer cells (Cullen, J. J., Hinkhouse, M. M., Grady, M., Gaut, A. W., Liu, J., Zhang, Y., Weydert, C. J. D., Domann, F. E., and Oberley, L. W. (2003) Cancer Res. 63,
Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow.
Journal:
PubMed ID:24385538
'How stem cells interact with the microenvironment to regulate their cell fates and metabolism is largely unknown. Here we demonstrated that the deletion of the cytoskeleton-modulating protein profilin 1 (pfn1) in hematopoietic stem cell (HSCs) led to bone marrow failure, loss of quiescence, and mobilization and apoptosis of HSCs in
KEAP1 is a redox sensitive target that arbitrates the opposing radiosensitive effects of parthenolide in normal and cancer cells.
Journal:
PubMed ID:23674500
'Elevated oxidative stress is observed more frequently in cancer cells than in normal cells. It is therefore expected that additional exposure to a low level of reactive oxygen species (ROS) will push cancer cells toward death, whereas normal cells might maintain redox homeostasis through adaptive antioxidant responses. We previously showed
G-protein ligands inhibit in vitro reactions of vacuole inheritance.
Journal:J Cell Biol
PubMed ID:8027189
'During budding in Saccharomyces cerevisiae, maternal vacuole material is delivered into the growing daughter cell via tubular or vesicular structures. One of the late steps in vacuole inheritance is the fusion in the bud of vesicles derived from the maternal vacuole. This process has been reconstituted in vitro and requires
A trace amount of the human pro-apoptotic factor Bax induces bacterial death accompanied by damage of DNA.
Journal:J Biol Chem
PubMed ID:9556634
'An amount of human pro-apoptotic Bax as low as 0.01% of total protein was sufficient to cause cell death in Escherichia coli. The bacterial cell death was examined using a viable bacteria-specific fluorescence indicator system and loss of colony formation ability. Co-expression of anti-apoptotic Bcl-xL showed a modest inhibitory effect