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Citations & References (26)
Invitrogen™
Coelenterazine h
Coelenterazine h is one of several synthetic coelenterazine analogs that confer different Ca2+ affinities and spectral properties on the aequorinRead more
| Catalog Number | Quantity |
|---|---|
| C6780 also known as C-6780 | 250 μg |
Catalog number C6780
also known as C-6780
Price (MXN)
-
Quantity:
250 μg
Coelenterazine h is one of several synthetic coelenterazine analogs that confer different Ca2+ affinities and spectral properties on the aequorin complex. Like native coelenterazine, these five novel derivatives can be used to reconstitute the aequorin complex both in vivo and in vitro.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodBioluminescence
Dye TypeProtein-Based
Quantity250 μg
Shipping ConditionRoom Temperature
For Use With (Equipment)Luminometer (Microplate), Luminometer (Tube-based)
Product TypeCoelenterazine
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (26)
Citations & References
Abstract
Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx.
Journal:J Cell Sci
PubMed ID:9427292
'We measured folate- and cAMP-induced changes in cytoplasmic free calcium concentration ([Ca2+]i) using recombinant aequorin reconstituted in living Dictyostelium cells with coelenterazine-h. The resulting semi-synthetic protein displayed increased sensitivity to Ca2+ allowing accurate measurement of chemoattractant-induced transients at low resting levels. Both folate- and cAMP-induced Ca2+ responses were developmentally regulated,
Quantitative assessment of beta 1- and beta 2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer.
Journal:J Biol Chem
PubMed ID:12244098
'Quantitative bioluminescence resonance energy transfer (BRET) analysis was applied to the study of beta(1)- and beta(2)-adrenergic receptor homo- and heterodimerization. To assess the relative affinity between each of the protomers, BRET saturation experiments were carried out in HEK-293T cells. beta(1)- and beta(2)-adrenergic receptors were found to have similar propensity to
The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate.
Journal:Biochem Biophys Res Commun
PubMed ID:9144537
'To investigate the use of various luciferases as reporter protein, the substrate specificity of recombinant Renilla luciferase, Oplophorus luciferase and recombinant apoaequorin was examined using 23 kinds of coelenterazine analogues as the substrate. The intensity of luminescence was generally highest with Oplophorus luciferase and lowest with apoaequorin, but varied widely
Characterization of coelenterazine analogs for measurements of Renilla luciferase activity in live cells and living animals.
Journal:Mol Imaging
PubMed ID:15142411
'In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes, luciferases, and delivery of chemical substrates to expressing cells. Coelenterazine (CLZN) is the substrate for a group of bioluminescent enzymes obtained from marine organisms. At present, there are more than 10 commercially available CLZN analogs. To determine which
Imaging [Ca2+]i with aequorin using a photon imaging detector.
Journal:Methods Cell Biol
PubMed ID:8201982