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MDM2 Monoclonal Antibody (OTI16H3), TrueMAB™

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MDM2 Monoclonal Antibody (OTI16H3), TrueMAB™

Product Details

CF804701

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI16H3

Immunogen

Human recombinant protein fragment corresponding to amino acids 119-438 of human MDM2 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alternatively spliced isoform; Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HGNC:6973; human homolog of; MDM2 isoform KB9; MDM2 isoform N1_40; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53 associated; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; unnamed protein product

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Gene Aliases: ACTFS; hdm2; HDMX; LSKB; MDM2

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UniProt ID: (Human) Q00987

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Entrez Gene ID: (Human) 4193

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Function(s)
p53 binding ubiquitin-protein transferase activity protein binding 5S rRNA binding zinc ion binding transferase activity ligase activity SUMO transferase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding receptor serine/threonine kinase binding identical protein binding peroxisome proliferator activated receptor binding ribonucleoprotein complex binding ubiquitin binding metal ion binding ubiquitin protein ligase activity NEDD8 ligase activity disordered domain specific binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination blood vessel development blood vessel remodeling regulation of heart rate heart valve development atrioventricular valve morphogenesis endocardial cushion morphogenesis ventricular septum development atrial septum development ubiquitin-dependent protein catabolic process apoptotic process traversing start control point of mitotic cell cycle heart development positive regulation of cell proliferation response to xenobiotic stimulus response to toxic substance response to iron ion regulation of gene expression positive regulation of gene expression negative regulation of gene expression negative regulation of protein processing negative regulation of neuron projection development protein ubiquitination protein sumoylation DNA damage response, signal transduction by p53 class mediator protein destabilization response to magnesium ion positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein localization to nucleus regulation of protein catabolic process response to cocaine negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of DNA damage response, signal transduction by p53 class mediator protein-containing complex subunit organization establishment of protein localization response to ether positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of mitotic cell cycle response to antibiotic positive regulation of protein export from nucleus response to steroid hormone positive regulation of muscle cell differentiation proteolysis involved in cellular protein catabolic process regulation of cell cycle protein autoubiquitination cardiac septum morphogenesis protein-containing complex assembly regulation of biological quality cellular response to hydrogen peroxide cellular response to antibiotic cellular response to vitamin B1 cellular response to alkaloid cellular response to growth factor stimulus cellular response to peptide hormone stimulus cellular response to estrogen stimulus cellular response to hypoxia cellular response to gamma radiation cellular response to UV-C signal transduction by p53 class mediator fibroblast activation cellular response to actinomycin D regulation of postsynaptic neurotransmitter receptor internalization regulation of protein catabolic process at postsynapse, modulating synaptic transmission negative regulation of signal transduction by p53 class mediator negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator response to formaldehyde positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration amyloid fibril formation response to water-immersion restraint stress negative regulation of apoptotic signaling pathway
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