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OriGene
EGLN2 Monoclonal Antibody (OTI7H3), TrueMAB™
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FIGURE: 1 / 7
EGLN2 Antibody (CF808584) in IHC (P)
Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-EGLN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808584)(1:150)
Product Details
CF808584
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI7H3
Immunogen
Full length human recombinant protein of human EGLN2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-alpha subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13. 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Egl nine homolog 2; EIT-6; Estrogen-induced tag 6; Falkor; HIF-1alpha prolyl-4-hydroxylase-1; HIF-P4H 1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; immediate early response 4; nuclear protein EIT-6; P4H1; PHD1; Prolyl hydroxylase domain-containing protein 1; Prolyl hydroxylase EGLN2; unnamed protein product
Gene Aliases: 0610011A13Rik; C85656; EGLN2; EIT-6; EIT6; Hif-p4h-1; HIF-PH1; HIFPH1; HPH-1; HPH-3; Ier4; PHD-1; PHD1; SM-20
UniProt ID: (Human) Q96KS0, (Mouse) Q91YE2, (Rat) Q6AYU4
Entrez Gene ID: (Human) 112398, (Mouse) 112406, (Rat) 308457
iron ion binding
protein binding
ferrous iron binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxygen sensor activity
L-ascorbic acid binding
peptidyl-proline 4-dioxygenase activity
metal ion binding
dioxygenase activity
hypoxia-inducible factor-proline dioxygenase activity
peptidyl-proline dioxygenase activity
regulation of cell growth
response to hypoxia
peptidyl-proline hydroxylation to 4-hydroxy-L-proline
intracellular estrogen receptor signaling pathway
regulation of neuron apoptotic process
cell redox homeostasis
positive regulation of protein catabolic process
cellular response to hypoxia
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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