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OriGene
SHMT2 Monoclonal Antibody (OTI5H10), TrueMAB™
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FIGURE: 1 / 1
SHMT2 Antibody (CF808846) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHMT2 (RC204239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SHMT2. Positive lysates LY401661 (100 µg) and LC401661 (20 µg) can be purchased separately from OriGene.
Product Details
CF808846
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI5H10
Immunogen
Full length human recombinant protein of human SHMT2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Mammalian serine hydroxymethyltransferase (SHMT) is a tetrameric, pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the reversible interconversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate in the cytoplasm (cSHMT, SHMT1) and mitochondria (mSHMT, SHMT2). cSHMT preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate, and inhibits SAM synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 51e; GLY A+; glycine auxotroph A, human complement for hamster; Glycine hydroxymethyltransferase; mitochondrial serine hydroxymethyltransferase; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); serine hydroxymethyltransferase 2 (mitochondrial) isoform SHMT2_1; serine hydroxymethyltransferase 2 (mitochondrial) isoform SHMT2_2; Serine hydroxymethyltransferase, mitochondrial; Serine methylase; SHMT; SHMT 2; threonine aldolase; unnamed protein product
Gene Aliases: 2700043D08Rik; AA408223; AA986903; GLYA; HEL-S-51e; mSHMT; NEDCASB; SHMT; SHMT2
UniProt ID: (Human) P34897, (Mouse) Q9CZN7
Entrez Gene ID: (Human) 6472, (Mouse) 108037, (Rat) 299857
regulation of oxidative phosphorylation
regulation of translation
glycine metabolic process
glycine biosynthetic process
L-serine metabolic process
L-serine biosynthetic process
one-carbon metabolic process
positive regulation of cell proliferation
formate biosynthetic process
glycine biosynthetic process from serine
response to type I interferon
tetrahydrofolate interconversion
tetrahydrofolate metabolic process
protein tetramerization
protein homotetramerization
regulation of mitochondrial translation
protein K63-linked deubiquitination
regulation of aerobic respiration
methylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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