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Invitrogen
Perlecan Monoclonal Antibody (A74)
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FIGURE: 1 / 1
Perlecan Antibody (CSI 001-74-02) in ELISA
BMC biotechnology 2010 -
Figure 2 ELISA identifies only Pln.D1 but not Pln.D3-5 in enriched rhPln.198 . The enriched high M r rhPln.198 synthesized by HEK 293 cells was used to coat microtiter wells that were subsequently blocked then incubated with 1.7 mug/ml of different primary antibodies as depicted in the legend. ELISA was completed as described in Methods. Background signal for each antibody binding to uncoated wells was subt... View More
Product Details
CSI 001-74-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
A74
Immunogen
Bovine corneal endothelial cells.
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Amount
428 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
CSI 001-74-02 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). CSI 001-74 cross-reacts with human perlecan. Other species have not been tested.
CSI 001-74-02 has been successfully used in ELISA, immunohistochemistry and Western blotting techniques. CSI 001-74-02 can be used in immunoprecipitation, ELISA and immunostaining of frozen PLP-fixed sections of bovine and human tissues. CSI 001-74-02 can be used in affinity chromatography, in conjunction with CSI 001-71-02 to separate recombinant domain I from full length perlecan.
Epitope of CSI 001-74-02 is located in domain V.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); HSPG; Perlecan; perlecan proteoglycan; PLC; putative proteoglycan; matrix protein; RP11-132G19.2
Gene Aliases: HSPG; HSPG2; PLC; PRCAN; SJA; SJS; SJS1
UniProt ID: (Human) P98160
Entrez Gene ID: (Bovine) 444872, (Human) 3339
angiogenesis
response to hypoxia
positive regulation of endothelial cell proliferation
lipid metabolic process
receptor-mediated endocytosis
inflammatory response
negative regulation of cell adhesion
smoothened signaling pathway
brain development
embryo implantation
negative regulation of cell proliferation
response to xenobiotic stimulus
negative regulation of angiogenesis
cell differentiation
organ regeneration
negative regulation of neuron apoptotic process
circulatory system development
negative regulation of amyloid fibril formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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