Synthesis and pharmacological characterization of novel fluorescent histamine H2-receptor ligands derived from aminopotentidine.
AuthorsXie SX, Petrache G, Schneider E, Ye QZ, Bernhardt G, Seifert R, Buschauer A,
JournalBioorg Med Chem Lett
PubMed ID16730977
'In an effort to develop a non-radioactive alternative to the [3H]tiotidine and [125I]iodoaminopotentidine binding assays for the histamine H2-receptor (H2R), primary amines related to aminopotentidine were prepared and coupled with the succinimidyl esters of the bulky fluorescent dyes S0536 and BODIPY 650/665-X. The primary amines exhibited different degrees of antagonistic ... More
Multicolor in vitro translation.
AuthorsTraverso G, Diehl F, Hurst R, Shuber A, Whitney D, Johnson C, Levin B, Kinzler KW, Vogelstein B
JournalNat Biotechnol
PubMed ID12910244
'In vitro translation is a widely used tool for both analytical and preparative purposes. For analytical purposes, small amounts of proteins are synthesized and visualized by detection of labeled amino acids incorporated during translation. The original strategy of incorporating radioactively labeled amino acids, such as [35S]methionine or [14C]leucine, has been ... More
Glycolipid trafficking in Drosophila undergoes pathway switching in response to aberrant cholesterol levels.
AuthorsHortsch R, Lee E, Erathodiyil N, Hebbar S, Steinert S, Lee JY, Chua DS, Kraut R,
JournalMol Biol Cell
PubMed ID20053687
In lipid storage diseases, the intracellular trafficking of sphingolipids is altered by conditions of aberrant cholesterol accumulation. Drosophila has been used recently to model lipid storage diseases, but the effects of sterol accumulation on sphingolipid trafficking are not known in the fly, and the trafficking of sphingolipids in general has ... More
Single-molecule detection and identification of multiple species by multiparameter fluorescence detection.
AuthorsWidengren J, Kudryavtsev V, Antonik M, Berger S, Gerken M, Seidel CA
JournalAnal Chem
PubMed ID16536444
Two general strategies are introduced to identify and quantify single molecules in dilute solutions by employing a spectroscopic method for data registration and specific burst analysis, denoted multiparameter fluorescence detection (MFD). MFD uses pulsed excitation and time-correlated single-photon counting to simultaneously monitor the evolution of the eight-dimensional fluorescence information (fundamental ... More
2,2'-thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy.
AuthorsStaudt T, Lang MC, Medda R, Engelhardt J, Hell SW
JournalMicrosc Res Tech
PubMed ID17131355
The use of high numerical aperture immersion lenses in optical microscopy is compromised by spherical aberrations induced by the refractive index mismatch between the immersion system and the embedding medium of the sample. Especially when imaging >10 micro m deep into the specimen, the refractive index mismatch results in a ... More
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry.
AuthorsSeo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J
JournalProc Natl Acad Sci U S A
PubMed ID15064393
DNA sequencing by synthesis on a solid surface offers new paradigms to overcome limitations of electrophoresis-based sequencing methods. Here we report DNA sequencing by synthesis using photocleavable (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3 alpha,4 alpha-diaza-s-indacene (Bodipy)-FL-510, dCTP-PC-Bodipy-650, and dUTP-PC-6-carboxy-X-rhodamine (ROX)] on a glass chip constructed by 1,3-dipolar azide-alkyne cycloaddition coupling chemistry. Each ... More