Improved retrograde axonal transport and subsequent visualization of tetramethylrhodamine (TMR) -dextran amine by means of an acidic injection vehicle and antibodies against TMR.
AuthorsKaneko T, Saeki K, Lee T, Mizuno N
JournalJ Neurosci Methods
PubMed ID8740593
'We studied the ability of various dextran amines (DA) to retrogradely label cortical neurons to the full extent of their dendritic configurations. Corticothalamic neurons were labeled by pressure injection of DA into the ventrobasal thalamic nuclei of the rat brain. Of fluorescein-, Texas Red-, Cascade Blue- and tetramethylrhodamine (TMR)-DAs of ... More
Evidence for differential cortical input to direct pathway versus indirect pathway striatal projection neurons in rats.
AuthorsLei W, Jiao Y, Del Mar N, Reiner A,
JournalJ Neurosci
PubMed ID15385612
'The two main types of corticostriatal neurons are those that project only intratelencephalically (IT-type), the intrastriatal terminals of which are 0.41 microm in mean diameter, and those that send their main axon into pyramidal tract and have a collateral projection to striatum (PT-type), the intrastriatal terminals of which are 0.82 ... More
Conjunction of vocal production and perception regulates expression of the immediate early gene ZENK in a novel cortical region of songbirds.
AuthorsBottjer SW, Alderete TL, Chang D,
JournalJ Neurophysiol
PubMed ID20107119
'The cortical nucleus LMAN (lateral magnocellular nucleus of the anterior nidopallium) provides the output of a basal ganglia pathway that is necessary for acquisition of learned vocal behavior during development in songbirds. LMAN is composed of two subregions, a core and a surrounding shell, that give rise to independent pathways ... More
Resolving the detailed structure of cortical and thalamic neurons in the adult rat brain with refined biotinylated dextran amine labeling.
AuthorsLing C, Hendrickson ML, Kalil RE,
JournalPLoS One
PubMed ID23144777
Biotinylated dextran amine (BDA) has been used frequently for both anterograde and retrograde pathway tracing in the central nervous system. Typically, BDA labels axons and cell somas in sufficient detail to identify their topographical location accurately. However, BDA labeling often has proved to be inadequate to resolve the fine structural ... More
Neuronal classification and marker gene identification via single-cell expression profiling of brainstem vestibular neurons subserving cerebellar learning.
AuthorsKodama T, Guerrero S, Shin M, Moghadam S, Faulstich M, du Lac S,
JournalJ Neurosci
PubMed ID22674258
Identification of marker genes expressed in specific cell types is essential for the genetic dissection of neural circuits. Here we report a new strategy for classifying heterogeneous populations of neurons into functionally distinct types and for identifying associated marker genes. Quantitative single-cell expression profiling of genes related to neurotransmitters and ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog.
AuthorsScalia F, Galoyan SM, Eisner S, Harris E, Su W
JournalJ Neurosci Methods
PubMed ID9350968
Anatomical study of the topographic organization of retinal projections requires a tracer capable of resolving fine morphological detail and permitting analysis of the projection from either the whole retina or selected areas. To obtain a permanent record of the experiments and to have access to ultrastructural data, it is preferable ... More
Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage.
AuthorsFujii R, Yamashita S, Hibi M, Hirano T
JournalJ Cell Biol
PubMed ID10995439
Cleavage is one of the initial steps of embryogenesis, and is characterized by a series of symmetric and synchronous cell divisions. We showed that p38 MAP kinase (p38) is asymmetrically activated on one side of the blastodisc during the early cleavage period in zebrafish (Danio rerio) embryos. When a dominant ... More
Improved Golgi-like visualization in retrogradely projecting neurons after EGFP-adenovirus infection in adult rat and monkey.
AuthorsTomioka R, Rockland KS
JournalJ Histochem Cytochem
PubMed ID16344324
An adenovirus vector was generated using a neuron-specific promoter synapsin I and enhanced green fluorescent protein (EGFP) reporter (AdSynEGFP). In addition, two modifications were identified that resulted in robust and reliable retrograde transport and EGFP expression after injection of the virus into three different brain regions in adult rats (medial ... More
Fiber pathways and positional changes in efferent perikarya of 2.5- to 7-day chick embryos as revealed with DiI and dextran amines.
AuthorsFritzsch B, Christensen MA, Nichols DH
JournalJ Neurobiol
PubMed ID7506749
The differentiation of facial motoneurons and inner ear (octaval) efferents was examined in chicken embryos by applying DiI or dextran amines to the cut VII/VIII nerve (peripheral label) or to the basal/floor plate of rhombomeres 4/5 (central label). Central labeling found axons of these efferent neurons to leave the brain ... More
Vital staining from dye-coated microprobes identifies new olfactory interneurons for optical and electrical recording.
AuthorsGelperin A, Flores J
JournalJ Neurosci Methods
PubMed ID9128173
A versatile technique for dye application in living tissue is described, which results in labeling of viable cells from which electrophysiological or optical recordings can be obtained. The dye-coated surface of a glass microelectrode tip is used to apply anatomical tracers or calcium sensitive probes with spatial precision. A total ... More
Potentiation of cerebellar Purkinje cells facilitates whisker reflex adaptation through increased simple spike activity.
Authors
JournalElife
PubMed ID30561331
Local Axonal Conduction Shapes the Spatiotemporal Properties of Neural Sequences.
Authors
JournalCell
PubMed ID33064989
Maintenance of Mouse Gustatory Terminal Field Organization Is Disrupted following Selective Removal of Peripheral Sodium Salt Taste Activity at Adulthood.
Authors
JournalJ Neurosci
PubMed ID28676575
Non-thalamic origin of zebrafish sensory nuclei implies convergent evolution of visual pathways in amniotes and teleosts.
Authors
JournalElife
PubMed ID32896272
The aqueous humor outflow pathway of zebrafish.
Authors
JournalInvest Ophthalmol Vis Sci
PubMed ID19060283
Modular output circuits of the fastigial nucleus for diverse motor and nonmotor functions of the cerebellar vermis.
Authors
JournalElife
PubMed ID32639229
Locomotion dependent neuron-glia interactions control neurogenesis and regeneration in the adult zebrafish spinal cord.
Authors
JournalNat Commun
PubMed ID34381039
Organization of primate amygdalar-thalamic pathways for emotions.
Authors
JournalPLoS Biol
PubMed ID32106269
Adult spinal motoneurons change their neurotransmitter phenotype to control locomotion.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID30275331
Acquisition of innate odor preference depends on spontaneous and experiential activities during critical period.