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Citations & References (86)
Invitrogen™
MQAE (N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide)
6-methoxyquinolinium derivative and fluorescent indicator for intracellular Cl-
| Catalog Number | Quantity |
|---|---|
| E3101 | 100 mg |
Catalog number E3101
Price (USD)
351.00
Each
Quantity:
100 mg
Price (USD)
351.00
Each
MQAE is a 6-methoxyquinolinium derivative and is a fluorescent indicator for intracellular Cl-. This dye detects the ion via diffusion-limited collisional quenching. MQAE has greater sensitivity to Cl- and a higher fluorescence quantum yield than SPQ; consequently, it is currently the more widely used of the two indicators.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeFluorescent Dye-Based
Emission Wavelength460 nm
Excitation Wavelength350 nm
Quantity100 mg
Shipping ConditionRoom Temperature
For Use With (Application)Chlorine Indicator
For Use With (Equipment)Confocal Microscope, Flow Cytometer
Product TypeMQAE
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (86)
Citations & References
Abstract
Noninvasive measurement of chloride concentration in rat olfactory receptor cells with use of a fluorescent dye.
Journal:Am J Physiol Cell Physiol
PubMed ID:11350733
Inwardly directed Ca(2+)-dependent chloride currents are thought to prolong and boost the odorant-induced transient receptor currents in olfactory cilia. Cl(-) inward current, of course, requires a sufficiently high intracellular Cl(-) concentration ([Cl(-)](i)). In previous measurements using a fluorescent Cl(-) probe, N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE), [Cl(-)](i) of newt olfactory cells was estimated
TRPV1 acts as proton channel to induce acidification in nociceptive neurons.
Journal:J Biol Chem
PubMed ID:15173182
The low extracellular pH of inflamed or ischemic tissues enhances painful sensations by sensitizing and activating the vanilloid receptor 1 (TRPV1). We report here that activation of TRPV1 results in a marked intracellular acidification in nociceptive dorsal root ganglion neurons and in a heterologous expression system. A characterization of the
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Determination of the intracellular free chloride concentration in rat brain synaptoneurosomes using a chloride-sensitive fluorescent indicator.
Journal:Biochim Biophys Acta
PubMed ID:8274496
'The chloride-sensitive fluorescent indicator MQAE (N-(6-methoxyquinolyl) acetoacetyl ester) has been used for determination of the intracellular free chloride concentration in rat brain synaptoneurosomes. Loading of the synaptoneurosomes with MQAE occurs by transmembrane diffusion. Calibration of the intracellular MQAE was done by determining the correlation between fluorescence intensity and intrasynaptoneurosomal Cl-
Effects of sasanquasaponin on ischemia and reperfusion injury in mouse hearts.
Journal:J Pharmacol Sci
PubMed ID:15037817
'We investigated effects of sasanquasaponin (SQS), a traditional Chinese herb''s effective component, on ischemia and reperfusion injury in mouse hearts and the possible role of intracellular Cl- homeostasis on SQS''s protective effects during ischemia and reperfusion. An in vivo experimental ischemia model was made in mice (weight 27-45 g) using