Invitrogen™

EnzChek™ Ultra Amylase Assay Kit

Catalog number: E33651
Invitrogen™

EnzChek™ Ultra Amylase Assay Kit

Catalog number: E33651
Catalog Number
E33651
Unit Size
1 kit
Price (USD)
302.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
E336511 kit
302.00
Your Price:
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Product Overview
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The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek™ kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY™ FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

EnzChek™ Ultra Amylase Assay Kit Specifications:
• Label (Ex/Em): BODIPY™ FL conjugate (∼502/512 nm)
• Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
• Sufficient reagents are supplied for 500 assays (using a 100 µL assay volume in a 96-well microplate assay format)


Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek™ Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more. Review Detecting Glycosidases—Section 10.2 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Fluorescence Intensity
For Use With (Application)
Ultra Amylase Assay
For Use With (Equipment)
Fluorescence Microplate Reader
Product Type
Amylase Assay
Product Line
EnzChek™
Shipping Condition
Room Temperature
Substrate Properties
Molecular Substrate
Substrate Type
Amylase Substrate
Target Enzyme
Amylase

Contents & Storage

Store at room temperature and protect from light.

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