Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR.
AuthorsSuzuki H, Murakami A, Yoshida K,
JournalAppl Environ Microbiol
PubMed ID22885745
'Counterselection systems facilitate marker-free genetic modifications in microbes by enabling positive selections for both the introduction of a marker gene into the microbe and elimination of the marker from the microbe. Here we report a counterselection system for Geobacillus kaustophilus HTA426, established through simultaneous disruption of the pyrF and pyrR ... More
PAR2 exerts local protection against acute pancreatitis via modulation of MAP kinase and MAP kinase phosphatase signaling.
AuthorsNamkung W, Yoon JS, Kim KH, Lee MG,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID18755806
'During acute pancreatitis, protease-activated receptor 2 (PAR2) can be activated by interstitially released trypsin. In the mild form of pancreatitis, PAR2 activation exerts local protection against intrapancreatic damage, whereas, in the severe form of pancreatitis, PAR2 activation mediates some systemic complications. This study aimed to identify the molecular mechanisms of ... More
Cell therapy for salivary gland regeneration.
AuthorsLin CY, Chang FH, Chen CY, Huang CY, Hu FC, Huang WK, Ju SS, Chen MH,
JournalJ Dent Res
PubMed ID21297017
'There are still no effective therapies for hyposalivation caused by irradiation. In our previous study, bone marrow stem cells can be transdifferentiated into acinar-like cells in vitro. Therefore, we hypothesized that transplantation with bone marrow stem cells or acinar-like cells may help functional regeneration of salivary glands. Bone marrow stem ... More
Pancreatic contamination of mesenteric adipose tissue samples can be avoided by adjusted dissection procedures.
AuthorsCaesar R, Drevon CA,
JournalJ Lipid Res
PubMed ID18408254
Mesenteric adipose tissue, located in the mesenterium of the intestines, is believed to play a central role in the development of obesity-related diseases. We have found that mesenteric fat samples harvested from rodents are frequently of poor quality, exhibiting partly degraded RNA. To investigate the background for this observation, we ... More
Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences.
AuthorsWatanabe K, Tsuchida Y, Okibe N, Teramoto H, Suzuki N, Inui M, Yukawa H,
JournalMicrobiology
PubMed ID19246745
Systematic screening of secretion proteins using an approach based on the completely sequenced genome of Corynebacterium glutamicum R revealed 405 candidate signal peptides, 108 of which were able to heterologously secrete an active-form alpha-amylase derived from Geobacillus stearothermophilus. These comprised 90 general secretory (Sec)-type, 10 twin-arginine translocator (Tat)-type and eight ... More
Induction of chimerism permits low-dose islet grafts in the liver or pancreas to reverse refractory autoimmune diabetes.
AuthorsZhang C, Wang M, Racine JJ, Liu H, Lin CL, Nair I, Lau J, Cao YA, Todorov I, Atkinson M, Zeng D,
JournalDiabetes
PubMed ID20530743
To test whether induction of chimerism lowers the amount of donor islets required for reversal of diabetes and renders the pancreas a suitable site for islet grafts in autoimmune diabetic mice. The required donor islet dose for reversal of diabetes in late-stage diabetic NOD mice after transplantation into the liver ... More
The gastrointestinal tract as a nutrient-balancing organ.
Failure to provision tissues with an appropriate balance of nutrients engenders fitness costs. Maintaining nutrient balance can be achieved by adjusting the selection and consumption of foods, but this may not be possible when the nutritional environment is limiting. Under such circumstances, rebalancing of an imbalanced nutrient intake requires post-ingestive ... More
Cholecystokinin-58 and cholecystokinin-8 exhibit similar actions on calcium signaling, zymogen secretion, and cell fate in murine pancreatic acinar cells.
AuthorsCriddle DN, Booth DM, Mukherjee R, McLaughlin E, Green GM, Sutton R, Petersen OH, Reeve JR,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID19815626
The gastrointestinal hormone CCK exists in various molecular forms, with differences in bioactivity between the well-characterized CCK-8 and larger CCK-58 previously reported. We have compared the effects of these peptides on cytosolic calcium concentration ([Ca(2+)](c)), mitochondrial metabolism, enzyme secretion, and cell fate in murine isolated pancreatic acinar cells using fluorescence ... More
Epithelial lining fluid solute concentrations in chronic obstructive lung disease patients and normal subjects.
AuthorsEffros RM, Peterson B, Casaburi R, Su J, Dunning M, Torday J, Biller J, Shaker R,
JournalJ Appl Physiol
PubMed ID15920100
The exhaled breath condensate (EBC) method represents a new, noninvasive way to detect inflammatory and metabolic markers in the fluid that covers the airways [epithelial lining fluid (ELF)]. However, respiratory droplets represent only a very small and variable fraction of the EBC, most (approximately 99.99%) of which is water vapor. ... More