Invitrogen™

NuPAGE™ 3-8% Tris-Acetate Protein Gels, 1.0 mm, 10-well

Catalog number:  EA0375PK2

Related applications:

Protein Electrophoresis & Western Blotting

Back to top

Description

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Adapters: No Adapters
For Use With (Equipment): Mini Gel Tank,XCell SureLock™ Mini
Gel Percentage: 3-8 %
Gel Size: Mini (8 cm x 8 cm)
Gel Type: Tris-Acetate Gel
Mode of Separation: Molecular Weight
Product Line: Novex™,NuPAGE®
Product Size: 2 gels (1 box)
Quantity: 2 gels
Recommended Applications: Denaturing,Native
Sample Loading Volume: up to 25 µL
Separation Range: 40 to 500 kDa (TA buffer)
Shipping Condition: Wet Ice
Thickness: 1.0 mm
Well Format: 1D Well
Wells: 10 wells

Contents & storage

One box contains 2 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 6 months.