HIF-1 alpha ELISA Kit, Human

Catalog number: EHIF1A

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific HIF-1 alpha ELISA Kit contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate. This product contains reagents for analyte detection in 96 microwells.

Features of Thermo Scientific HIF-1 alpha ELISA Kits:†

Target: natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 81.92 to 20,000 pg/mL
Sensitivity: < 30 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture lysate, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50 µL
Specificity: specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A.

† Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

These HIF-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of hypoxia-inducible factor 1-alpha (HIF1A, also called BHLHE78, MOP1, PASD8) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Human
Product Size: 96 assays
Protein Family: Transcription Factors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols