Leukotriene B4 Competitive ELISA Kit, Multispecies

Catalog number: EHLTB4

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Assay LTB4 (leukotriene B4) eicosanoid levels in biological samples, such as tissue culture media, plasma, saliva and urine, with our Thermo Scientific Leukotriene B4 Competitive ELISA Kit. This complete kit contains pre-coated antibody-plate, detection antibodies, diluent, wash buffer, standard, substrate, and stop solution.

Features of the Leukotriene B4 ELISA Kit:

Target: leukotriene B4 (LTB4, LT-B4)
Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
Measurement: absorbance at 405nm minus absorbance at 570nm
Assay Range: 47 to 12,000 pg/mL
Sensitivity: < 19.4 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell culture supernatant, serum, plasma (EDTA), saliva, urine
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with PGE2,PGF2α, 20-OH-LTB4, 20-COOH-LTB4, LTC4, LTD4, LTE4, 5(S)-HETE, 12(S)-HETE, 15(S)-HETE; less than 1% cross-reaction with 12-epi-LTB4; approx. 5% cross-reaction with 6-trans-LTB4 and 6-trans-12-epi-LTB4.

This LTB4 competitive ELISA kit is an immunoassay for the quantitative determination of leukotriene B4 (LTB4) in biological fluids. The assay is based on the competition between LTB4 in the standard or sample and alkaline phosphatase conjugated LTB4 (LTB4-AP) for a limited amount of LTB4 monoclonal antibody bound to an anti-Rabbit IgG precoated 96-well plate. As the concentration of LTB4 in the sample increases, the amount of LTB4-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Multiple
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Inflammation

Contents & storage

Sufficient For: 96 microplate wells
• Anti-Rabbit IgG Precoated 96-well Plate, 1 each
• Reagent Diluent, 30 mL
• LTB4 Antibody, 6 mL
• LTB4-AP Conjugate, 6 mL
• LTB4 Standard, 0.5 mL
• 10X Wash Buffer, 30 mL
• Substrate Solution, 20 mL
• Stop Solution, 6 mL
• Plate Sealer, 1 each
• LTB4 Assay Layout Sheet, 1 each

Documents

Manuals & protocols