Prostaglandin E2 Competitive ELISA Kit, Multispecies

Catalog number: EHPGE2

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Assay PGE2 (prostaglandin E2) levels in biological samples, such as tissue culture media, plasma, saliva and urine, with our Thermo Scientific Prostaglandin E2 Competitive ELISA Kit.

Features of the Prostaglandin E2 Competitive ELISA Kit:

Target: prostaglandin E2 (PGE2)
Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
Measurement: absorbance at 405nm minus absorbance at 570nm
Assay Range: 39 to 5000 pg/mL
Sensitivity: < 15.9 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell culture supernatant, serum, plasma (EDTA), whole blood, saliva, urine
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with 13,14-dihydro-15-keto-PGF2a; 6,15-keto-13-dihydro-PGF1a; Thromboxane B2; 2-Arachidonoylglycerol; Anandamide; PGD2; Arachadonic acid. Less than 1% cross-reaction with PFG2a; 6-keto-PGF1a; PGA2; PGB1; Approx. 16% cross-reaction with PGE3; Approx. 70% cross-reaction with PGE1.

This PGE2 competitive ELISA kit is an immunoassay for the quantitative determination of prostaglandin E2 (PGE2) in biological fluids. The assay is based on the competition between PGE2 in the standard or sample and alkaline phosphatase conjugated PGE2 (PGE2-AP) for a limited amount of PGE2 monoclonal antibody bound to an anti-mouse IgG precoated 96-well plate. As the concentration of PGE2 in the sample increases, the amount of PGE2-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.

Includes:
Complete kit contains pre-coated antibody-plate, detection antibodies, diluent, wash buffer, standard, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Multiple
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Sufficient For: 96 microplate wells
• Anti-Mouse IgG Precoated 96-well Plate, 1 each
• Reagent Diluent, 30 mL
• PGE2 Antibody, 6 mL
• PGE2-AP Conjugate, 6 mL
• PGE2 Standard, 0.55 mL
• 10X Wash Buffer, 30 mL
• Substrate Solution 20 mL
• Stop Solution, 6 mL
• Plate Sealer, 1 each
• PGE2 Assay Layout Sheet, 1 each

Documents

Manuals & protocols