RANTES Human ELISA Kit

Catalog number: EHRNTS5

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay RANTES (regulated upon activation, normal T-cell expressed and secreted) levels in human serum, plasma, tissue extracts, urine and culture supernatant samples with our Thermo Scientific Human RANTES ELISA Kits.

Features of the Human RANTES ELISA Kit:

Target: natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450 nm minus absorbance at 550nm
Assay Range: 51.2 to 2000 pg/mL
Sensitivity: < 2 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human Eotaxin, GROα, GROβ, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFα or TNFβ, or rat RANTES.

These human RANTES ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of RANTES (regulated upon activation, normal T-cell expressed and secreted) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Chemokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP
Gene ID: 6352
Gene Name: C-C motif chemokine ligand 5
Gene Symbol: CCL5
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Name: C-C motif chemokine 5
Target (Kit Named): RANTES
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P13501

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols