Cercarial Elastase Is Encoded by a Functionally Conserved Gene Family across Multiple Species of Schistosomes.
AuthorsSalter Jason P.; Choe Youngchool; Albrecht Hugo; Franklin Christopher; Lim Kee-Chong; Craik Charles S.; McKerrow James H.;
JournalJ Biol Chem
PubMed ID11986325
Water borne cercaria(ae) of the trematode genus Schistosoma rapidly penetrate host skin. A single serine protease activity, cercarial elastase, is deposited in advance of the invading parasite by holocytosis of vesicles from ten large acetabular gland cells. Cercarial elastase activity is a composite of multiple isoforms. Genes coding for the ... More
Definition of genetically distinct attenuation mechanisms in naturally virulence-attenuated Listeria monocytogenes by comparative cell culture and molecular characterization.
AuthorsRoberts A, Chan Y, Wiedmann M,
JournalAppl Environ Microbiol
PubMed ID16000803
'Listeria monocytogenes is a foodborne pathogen able to cause serious disease in humans and animals. Not all isolates are equally pathogenic, however, and several isolates have been characterized as naturally virulence attenuated. We sought to identify the genetic basis of natural virulence attenuation using cell culture assays and molecular techniques. ... More
Combined effect of epinephrine and exercise on calpain/calpastatin and cathepsin B and L activity in porcine longissimus muscle.
AuthorsErtbjerg P, Henckel P, Karlsson A, Larsen LM, Møller AJ,
JournalJ Anim Sci
PubMed ID10492449
The objective of the study was to improve the understanding of the relationship between the effect of epinephrine plus exercise and meat tenderness. The calpain, calpastatin, and cathepsin B + L activities and postmortem proteolysis in porcine longissimus muscle were studied. The muscle glycogen stores were depleted in five pigs ... More
Regulation of the mitogen-activated protein kinase p44 ERK activity during anoxia/recovery in rainbow trout hypodermal fibroblasts.
AuthorsOssum CG, Wulff T, Hoffmann EK,
JournalJ Exp Biol
PubMed ID16621957
It is well known from various mammalian cells that anoxia has a major impact on the mitogen-activated protein kinase ERK, but a possible similar effect in fish cells has not been investigated. Here we characterise a p44ERK-like protein in the rainbow trout cell line RTHDF and study the effect of ... More
Separate basic region motifs within the adeno-associated virus capsid proteins are essential for infectivity and assembly.
AuthorsGrieger JC, Snowdy S, Samulski RJ,
JournalJ Virol
PubMed ID16699000
Adeno-associated virus (AAV) is gaining momentum as a gene therapy vector for human applications. However, there remain impediments to the development of this virus as a vector. One of these is the incomplete understanding of the biology of the virus, including nuclear targeting of the incoming virion during initial infection, ... More
Neuropeptide Y Y4 receptor homodimers dissociate upon agonist stimulation.
AuthorsBerglund MM, Schober DA, Esterman MA, Gehlert DR,
JournalJ Pharmacol Exp Ther
PubMed ID14551289
The pancreatic polypeptide-fold family of peptides consists of three 36-amino acid peptides, namely neuropeptide Y (NPY), peptide YY, and pancreatic polypeptide (PP). These peptides regulate important functions, including food intake, circadian rhythms, mood, blood pressure, intestinal secretion, and gut motility, through four receptors: Y1, Y2, Y4, and Y5. Additional receptor ... More
Surface-exposed adeno-associated virus Vp1-NLS capsid fusion protein rescues infectivity of noninfectious wild-type Vp2/Vp3 and Vp3-only capsids but not that of fivefold pore mutant virions.
AuthorsGrieger JC, Johnson JS, Gurda-Whitaker B, Agbandje-McKenna M, Samulski RJ,
JournalJ Virol
PubMed ID17507473
Over the past 2 decades, significant effort has been dedicated to the development of adeno-associated virus (AAV) as a vector for human gene therapy. However, understanding of the virus with respect to the functional domains of the capsid remains incomplete. In this study, the goal was to further examine the ... More