XCell II™ Blot Module
XCell II™ Blot Module
인용 및 참조 문헌 (7)
Invitrogen™

XCell II™ Blot Module

The XCell II Blot Module is a mini-gel wet-transfer module for the XCell SureLock or XCell II systems. The XCell자세히 알아보기
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카탈로그 번호수량
EI90511 unit
카탈로그 번호 EI9051
제품 가격(KRW)
777,000
온라인 행사
Ends: 31-Mar-2026
888,000
할인액 111,000 (13%)
Each
카트에 추가하기
수량:
1 unit
제품 가격(KRW)
777,000
온라인 행사
Ends: 31-Mar-2026
888,000
할인액 111,000 (13%)
Each
카트에 추가하기
The XCell II Blot Module is a mini-gel wet-transfer module for the XCell SureLock or XCell II systems. The XCell II Blot Module allows you to easily transfer proteins or nucleic acids for western, southern, and northern transfers from mini gels to membranes with less than 200 mL of transfer buffer. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.

See all available transfer systems ›

For Research Use Only. Not for use in diagnostic procedures.
사양
용량Up to 2 mini-gels
용도(장비)XCell SureLock™ Mini and Mini Gel Tank
젤 크기Mini (8 cm x 8 cm)
이송 모드Wet
수량1 unit
작동 치수Vertical
배송 조건Room Temperature
Gel CompatibilityBolt Bis-Tris Plus Gel, NuPAGE Gel, Novex Mini Gel
제품라인XCell II
제품 유형Blot Module
Unit SizeEach
구성 및 보관
The XCell II™ Blot Module includes anode core, cathode core, sponge pads (8/pack), and instruction manual.

자주 묻는 질문(FAQ)

Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I improve transfer efficiency for larger proteins during western blotting?

Here are some options for obtaining more efficient transfer for larger proteins:

1) Pre-equilibrate the gel with 0.02 to 0.04% SDS in 2X transfer buffer without methanol for 10 min before assembling the sandwich.

2) Increase the blotting time incrementally (in 15 min intervals).

3) Add 0.01% or 0.02% SDS to the transfer buffer to help facilitate the migration of the protein out of the gel.

4) Decrease the methanol content in the transfer buffer.

5) Switch to a more appropriate lower-percentage gel. A lower-percentage gel may allow better transfer than a higher-percentage gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do I have to run the XCell Blot Module under cold conditions?

No. The solution placed in the outer chamber serves to dissipate the heat generated during blotting. Water is usually used for this purpose. The recommended transfer conditions generate only a minor heat increase, so it is not necessary to run the unit in an ice bucket or to place it in a cold room. However if you are working with very heat-sensitive proteins, you may wish to do so.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I remove residual build-up in the XCell II Blot Module?

Build-up can be removed with 50% nitric acid. Make a solution of 50% nitric acid in deionized water and carefully apply it to areas inside the blot module until residual build-up is removed. Do not submerge the blot module or soak overnight. Use gloves when preparing the solution. Afterwards, rinse the module thoroughly at least three times in fresh deionized water. This treatment should not harm the plastic.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can residual build-up in the XCell II Blot Module be removed?

Build-up can be removed with 50% nitric acid. Make a solution of 50% nitric acid in deionized water and carefully apply it to areas inside the blot module until residual build-up is removed. Do not submerge the blot module or soak overnight. Use gloves when preparing the solution. Afterwards, rinse the module thoroughly at least three times in fresh deionized water. This treatment should not harm the plastic.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all XCell II™ Blot Module FAQs

인용 및 참조 문헌 (7)

인용 및 참조 문헌
Abstract
Cercarial Elastase Is Encoded by a Functionally Conserved Gene Family across Multiple Species of Schistosomes.
Authors:Salter Jason P.; Choe Youngchool; Albrecht Hugo; Franklin Christopher; Lim Kee-Chong; Craik Charles S.; McKerrow James H.;
Journal:J Biol Chem
PubMed ID:11986325
Water borne cercaria(ae) of the trematode genus Schistosoma rapidly penetrate host skin. A single serine protease activity, cercarial elastase, is deposited in advance of the invading parasite by holocytosis of vesicles from ten large acetabular gland cells. Cercarial elastase activity is a composite of multiple isoforms. Genes coding for the ... More
Definition of genetically distinct attenuation mechanisms in naturally virulence-attenuated Listeria monocytogenes by comparative cell culture and molecular characterization.
Authors:Roberts A, Chan Y, Wiedmann M,
Journal:Appl Environ Microbiol
PubMed ID:16000803
'Listeria monocytogenes is a foodborne pathogen able to cause serious disease in humans and animals. Not all isolates are equally pathogenic, however, and several isolates have been characterized as naturally virulence attenuated. We sought to identify the genetic basis of natural virulence attenuation using cell culture assays and molecular techniques. ... More
Combined effect of epinephrine and exercise on calpain/calpastatin and cathepsin B and L activity in porcine longissimus muscle.
Authors:Ertbjerg P, Henckel P, Karlsson A, Larsen LM, Møller AJ,
Journal:J Anim Sci
PubMed ID:10492449
The objective of the study was to improve the understanding of the relationship between the effect of epinephrine plus exercise and meat tenderness. The calpain, calpastatin, and cathepsin B + L activities and postmortem proteolysis in porcine longissimus muscle were studied. The muscle glycogen stores were depleted in five pigs ... More
Regulation of the mitogen-activated protein kinase p44 ERK activity during anoxia/recovery in rainbow trout hypodermal fibroblasts.
Authors:Ossum CG, Wulff T, Hoffmann EK,
Journal:J Exp Biol
PubMed ID:16621957
It is well known from various mammalian cells that anoxia has a major impact on the mitogen-activated protein kinase ERK, but a possible similar effect in fish cells has not been investigated. Here we characterise a p44ERK-like protein in the rainbow trout cell line RTHDF and study the effect of ... More
Separate basic region motifs within the adeno-associated virus capsid proteins are essential for infectivity and assembly.
Authors:Grieger JC, Snowdy S, Samulski RJ,
Journal:J Virol
PubMed ID:16699000
Adeno-associated virus (AAV) is gaining momentum as a gene therapy vector for human applications. However, there remain impediments to the development of this virus as a vector. One of these is the incomplete understanding of the biology of the virus, including nuclear targeting of the incoming virion during initial infection, ... More
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