Inhibition of phosphoinositide 3-kinase delta attenuates allergic airway inflammation and hyperresponsiveness in murine asthma model.
AuthorsLee KS, Lee HK, Hayflick JS, Lee YC, Puri KD
JournalFASEB J
PubMed ID16507763
'P110delta phosphoinositide 3-kinase (PI3K) plays a pivotal role in the recruitment and activation of certain inflammatory cells. Recent findings revealed that the activity of p110delta also contributes to allergen-IgE-induced mast cell activation and vascular permeability. We investigated the role of p110delta in allergic airway inflammation and hyperresponsiveness using IC87114, a ... More
Retroviral gene therapy with an immunoglobulin-antigen fusion construct protects from experimental autoimmune uveitis.
AuthorsAgarwal RK, Kang Y, Zambidis E, Scott DW, Chan CC, Caspi RR
JournalJ Clin Invest
PubMed ID10903340
'Immunoglobulins can serve as tolerogenic carriers for antigens, and B cells can function as tolerogenic antigen-presenting cells. We used this principle to design a strategy for gene therapy of experimental autoimmune uveitis, a cell-mediated autoimmune disease model for human uveitis induced with the uveitogenic interphotoreceptor retinoid-binding protein (IRBP). A retroviral ... More
Prevention of type I diabetes transfer by glutamic acid decarboxylase 65 peptide 206-220-specific T cells.
AuthorsKim SK, Tarbell KV, Sanna M, Vadeboncoeur M, Warganich T, Lee M, Davis M, McDevitt HO
JournalProc Natl Acad Sci U S A
PubMed ID15381770
'Glutamic acid decarboxylase (GAD) 65 is one of the major pancreatic antigens targeted by self-reactive T cells in type I diabetes mellitus. T cells specific for GAD65 are among the first to enter inflamed islets and may be important for the initiation of autoimmune diabetes. However, we previously reported that ... More
Airborne Asian sand dust enhances murine lung eosinophilia.
AuthorsHe M, Ichinose T, Yoshida S, Nishikawa M, Mori I, Yanagisawa R, Takano H, Inoue K, Sun G, Shibamoto T
JournalInhal Toxicol
PubMed ID20849355
'There is no experimental study demonstrating the effects of airborne Asian sand dust (AASD) on allergic lung eosinophilia. The organic substances adsorbed onto AASD collected from the atmosphere of Iki-island in Japan were excluded by heat treatment at 360°C for 30 min. The effects of AASD or heated-AASD (H-AASD) towards ... More
Disruption of antigen-induced airway inflammation and airway hyper-responsiveness in low affinity neurotrophin receptor p75 gene deficient mice.
AuthorsTokuoka S, Takahashi Y, Masuda T, Tanaka H, Furukawa S, Nagai H
JournalBr J Pharmacol
PubMed ID11724766
'1. Recently, much attention has been paid to the relationship between the nervous and immune systems. The present study was conducted to clarify the role of neurotrophin low affinity receptor (p75N) in allergic airway inflammation and hyper-responsiveness (AHR) in mice by employing p75N gene deficient mice. 2. Mice were immunized ... More
The role of Foxp3+ T cells in long-term efficacy of prophylactic and therapeutic mucosal tolerance induction in mice.
AuthorsWinkler B, Hufnagl K, Spittler A, Ploder M, Kállay E, Vrtala S, Valenta R, Kundi M, Renz H, Wiedermann U
JournalAllergy
PubMed ID16409192
'Mucosal tolerance induction is suggested as treatment strategy for allergic diseases. Using a murine model of birch pollen (BP) allergy we investigated the long-term efficacy and the underlying mechanisms of mucosal tolerance induction with two structurally different molecules in a prophylactic and in a therapeutic set-up.' ... More
Transforming growth factor beta blocks Tec kinase phosphorylation, Ca2+ influx, and NFATc translocation causing inhibition of T cell differentiation.
AuthorsChen CH, Seguin-Devaux C, Burke NA, Oriss TB, Watkins SC, Clipstone N, Ray A
JournalJ Exp Med
PubMed ID12810687
'Transforming growth factor (TGF)-beta inhibits T cell proliferation and differentiation. TGF-beta has been shown to inhibit the expression of transcription factors such as GATA-3 and T-bet that play important roles in T cell differentiation. Here we show that TGF-beta inhibits T cell differentiation at a more proximal step. An early ... More
Mucosal co-application of lactic acid bacteria and allergen induces counter-regulatory immune responses in a murine model of birch pollen allergy.
AuthorsRepa A, Grangette C, Daniel C, Hochreiter R, Hoffmann-Sommergruber K, Thalhamer J, Kraft D, Breiteneder H, Mercenier A, Wiedermann U
JournalVaccine
PubMed ID14604575
'Recent epidemiological studies and clinical trials suggest a possible role of certain lactic acid bacteria (LAB) strains in the prevention of allergic diseases. In this study, we aimed at evaluating the immunomodulatory potential of two LAB strains, Lactococcus lactis and Lactobacillus plantarum, for prophylaxis and therapy of allergic immune responses. ... More
The effects of microbial materials adhered to Asian sand dust on allergic lung inflammation.
AuthorsIchinose T, Yoshida S, Hiyoshi K, Sadakane K, Takano H, Nishikawa M, Mori I, Yanagisawa R, Kawazato H, Yasuda A, Shibamoto T
JournalArch Environ Contam Toxicol
PubMed ID18227959
'Asian sand dust (ASD) containing microbiological materials, sulfate (SO(4)(2)), and nitrate (NO(3)(-) ) derived from air pollutants in East China, reportedly cause adverse respiratory health effects. ASD aggravates ovalbumin (OVA)-associated experimental lung eosinophilia. In this study, the toxic materials adsorbed onto ASD were excluded by heat treatment at 360 degrees ... More
Resting B cells are not antigen-presenting cells in the induction of oral tolerance of specific Th2 immune responses in mice.
AuthorsPeng HJ, Chang ZN, Kuo SW, Lee CC, Tzau YY
JournalInt Arch Allergy Immunol
PubMed ID10899760
It has been shown that antigen presentation by resting B cells can induce tolerance to intravenously administered protein antigens, but the role of resting B cells in the induction of oral tolerance is unclear. ... More
Intranasal treatment with a recombinant hypoallergenic derivative of the major birch pollen allergen Bet v 1 prevents allergic sensitization and airway inflammation in mice.
AuthorsWiedermann U, Herz U, Baier K, Vrtala S, Neuhaus-Steinmetz U, Bohle B, Dekan G, Renz H, Ebner C, Valenta R, Kraft D
JournalInt Arch Allergy Immunol
PubMed ID11641608
The major birch pollen allergen Bet v 1 represents one of the most prevalent environmental allergens responsible for allergic airway inflammation.
Uteroglobin-related protein 1 expression suppresses allergic airway inflammation in mice.
AuthorsChiba Y, Kurotani R, Kusakabe T, Miura T, Link BW, Misawa M, Kimura S
JournalAm J Respir Crit Care Med
PubMed ID16456148
Uteroglobin-related protein (UGRP) 1, which is highly expressed in the epithelial cells of the airways, has been suggested to play a role in lung inflammation.
Oral administration of an edible-mushroom-derived protein inhibits the development of food-allergic reactions in mice.
AuthorsHsieh KY, Hsu CI, Lin JY, Tsai CC, Lin RH
JournalClin Exp Allergy
PubMed ID14616874
Food allergy is a common disease without effective treatment. Since strict elimination of food allergens may be difficult, strategies for effective intervention are urgently needed.
IL-5 deficiency abolishes aspects of airway remodelling in a murine model of lung inflammation.
AuthorsTrifilieff A, Fujitani Y, Coyle AJ, Kopf M, Bertrand C
JournalClin Exp Allergy
PubMed ID11422160
Lung remodelling is a recognized feature of chronic asthma. In the present study, we have used IL-5-deficient mice to evaluate the role of this cytokine and eosinophilic inflammation in the initial stages of the structural changes occurring in the lung after antigen challenge. ... More
Differential roles for CD4 and CD8 T cells after diisocyanate sensitization: genetic control of TH2-induced lung inflammation.
AuthorsHerrick CA, Das J, Xu L, Wisnewski AV, Redlich CA, Bottomly K
JournalJ Allergy Clin Immunol
PubMed ID12743574
Exposure to diisocyanates is a major cause of occupational asthma. We previously developed a novel mouse model of diisocyanate-induced asthma involving epicutaneous sensitization to hexamethylene diisocyanate (HDI) that demonstrates many features of the human disease, including airway eosinophilia and mucus hypersecretion. ... More
A recombinant allergen chimer as novel mucosal vaccine candidate for prevention of multi-sensitivities.
AuthorsWild C, Wallner M, Hufnagl K, Fuchs H, Hoffmann-Sommergruber K, Breiteneder H, Scheiner O, Ferreira F, Wiedermann U
JournalAllergy
PubMed ID17156339
As conventional immunotherapy is less efficacious in patients with allergic multi-sensitivities compared with mono-sensitized subjects, new intervention strategies are needed. Therefore, an allergen chimer was genetically engineered for treatment of multi-sensitization with birch and grass pollen on the basis of mucosal tolerance induction. ... More
Asian pear pectin administration during presensitization inhibits allergic response to ovalbumin in BALB/c mice.
AuthorsLee JC, Pak SC, Lee SH, Na CS, Lim SC, Song CH, Bai YH, Jang CH
JournalJ Altern Complement Med
PubMed ID15253858
A type of respiratory disorder resembling some aspects of human allergic asthma can be induced in mice using ovalbumin. The factors that influence the etiology of asthma are poorly understood even though cytokines are known to play a pivotal role. The purpose of this study was to test the hypothesis ... More
Involvement of CCR3-reactive chemokines in eosinophil survival.
AuthorsShinagawa K, Trifilieff A, Anderson GP
JournalInt Arch Allergy Immunol
PubMed ID12673069
Although eosinophils undergo apoptosis and are thus eliminated from sites of inflammation, they survive longer if survival factors, such as IL-3, IL-5 and GM-CSF, are present. However, it is often observed that some eosinophils survive even when incubated without any survival factors (spontaneous survival). The aim of the present study ... More
In vivo DNA electrotransfer into muscle.
AuthorsMiyazaki S, Miyazaki J
JournalDev Growth Differ
PubMed ID18445065
Naked plasmid DNA injected into skeletal muscle is taken up by muscle cells and the genes in the plasmid are expressed. Among the non-viral techniques for gene transfer in vivo, this method is especially simple, inexpensive, and safe. However, the relatively low expression levels attained by this method have limited ... More
Epigallocatechin-3-gallate protects toluene diisocyanate-induced airway inflammation in a murine model of asthma.
AuthorsKim SH, Park HJ, Lee CM, Choi IW, Moon DO, Roh HJ, Lee HK, Park YM
JournalFEBS Lett
PubMed ID16516891
Epigallocatechin-3-gallate (EGCG), a major form of tea catechin, has anti-allergic properties. To elucidate the anti-allergic mechanisms of EGCG, we investigated its regulation of matrix metalloproteinase (MMP-9) expression in toluene diisocyanate (TDI)-inhalation lung tissues as well as TNF-alpha and Th2 cytokine (IL-5) production in BAL fluid. Compared with untreated asthmatic mice ... More
B7/CD28 costimulation is essential for the homeostasis of the CD4+CD25+ immunoregulatory T cells that control autoimmune diabetes.
AuthorsSalomon B, Lenschow DJ, Rhee L, Ashourian N, Singh B, Sharpe A, Bluestone JA
JournalImmunity
PubMed ID10795741
CD28/B7 costimulation has been implicated in the induction and progression of autoimmune diseases. Experimentally induced models of autoimmunity have been shown to be prevented or reduced in intensity in mice rendered deficient for CD28 costimulation. In sharp contrast, spontaneous diabetes is exacerbated in both B7-1/B7-2-deficient and CD28-deficient NOD mice. These ... More
Immunization by Arg-gingipain A DNA vaccine protects mice against an invasive Porphyromonas gingivalis infection through regulation of interferon-gamma production.
AuthorsYonezawa H, Kato T, Kuramitsu HK, Okuda K, Ishihara K
JournalOral Microbiol Immunol
PubMed ID16101960
We previously demonstrated that a Porphyromonas gingivalis rgpA DNA vaccine induced protective immune responses against P. gingivalis infection in mice. In the present study, reduction in lethality against infection by lethal doses of P. gingivalis was observed in the rgpA DNA vaccine-immunized mice. Cytokine levels in the mouse model with ... More
Influence of maternal immunity on antibody and T-cell response in mice.
AuthorsBlomqvist GA, Lövgren-Bengtsson K, Morein B
JournalVaccine
PubMed ID12706692
The prevalence of maternal antibodies (Abs) and an immature neonate immune system, which is inclined to a T-helper 2 (Th2) response, are factors that counteract active immunization in early life. In a mouse model, the maternal influence on an active immunization of 2-day-old offspring with Sendai virus (SV) envelope proteins ... More
SCID mice show a similar susceptibility to Angiostrongylus cantonensis as do wild-type mice of the C.B-17 strain.
AuthorsYoshimura K, Sugaya H, Aoki M, Ishida K
JournalParasitol Res
PubMed ID10935903
C.B-17-SCID/SCID (SCID) mice infected with Angiostrongylus cantonensis yielded a high percentage of worm recovery and did not show any body weight loss until day 24 postinfection. Unexpectedly, C.B-17-+/+(+/+) mice also produced a similar worm burden containing well-developed worms. This is probably attributable to the observation that +/+ mice failed to ... More
Protective immunity induced by the full-length cDNA encoding paramyosin of Chinese Schistosoma japonicum.
AuthorsZhou S, Liu S, Song G, Xu Y, Sun W
JournalVaccine
PubMed ID10856799
The full-length cDNA encoding paramyosin of Chinese S. japonicum (Sjc97) has been cloned and sequenced for the first time. The homology of the nucleotide sequence of paramyosin and the deduced amino acid sequence between Chinese, Philippine, Japanese strains and S. mansoni (Sm97) were 99.7, 99.8 and 96% at amino acid ... More
Overlapping roles for granulocyte-macrophage colony-stimulating factor and interleukin-3 in eosinophil homeostasis and contact hypersensitivity.
AuthorsGillessen S, Mach N, Small C, Mihm M, Dranoff G
JournalBlood
PubMed ID11159518
Studies of mice rendered deficient in granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3) have established unique roles for these cytokines in pulmonary homeostasis, resistance to infection, and antigen-specific T- and B-cell responses. In addition to these distinctive properties, however, GM-CSF and IL-3 also stimulate the development and activation of hematopoietic ... More
Allergic diathesis in transgenic mice with constitutive T cell expression of inducible vasoactive intestinal peptide receptor.
AuthorsVoice JK, Dorsam G, Lee H, Kong Y, Goetzl EJ
JournalFASEB J
PubMed ID11689474
Vasoactive intestinal peptide (VIP) and its G-protein-coupled receptors (VPAC1 and VPAC2 Rs) are prominent in the immune system. In T cells, VPAC1 R is expressed constitutively whereas VPAC2 R is induced only after stimulation of the T cell receptor (TCR) or exposure to some cytokines. VPAC1 R and VPAC2 R ... More
Blockade of transforming growth factor beta/Smad signaling in T cells by overexpression of Smad7 enhances antigen-induced airway inflammation and airway reactivity.
AuthorsNakao A, Miike S, Hatano M, Okumura K, Tokuhisa T, Ra C, Iwamoto I
JournalJ Exp Med
PubMed ID10899902
Transforming growth factor (TGF)-beta has been implicated in immunosuppression. However, it remains obscure whether regulation of T cells by TGF-beta contributes to the immunosuppression in vivo. To address this issue, we developed transgenic mice expressing Smad7, an intracellular antagonist of TGF-beta/Smad signaling, selectively in mature T cells using a plasmid ... More
Epitope-specific antibody response to Mel-CAM induced by mimotope immunization.
AuthorsHafner C, Wagner S, Jasinska J, Allwardt D, Scheiner O, Wolff K, Pehamberger H, Wiedermann U, Breiteneder H
JournalJ Invest Dermatol
PubMed ID15654965
Peptide mimotopes of tumor antigen epitopes have been proposed as components of tumor vaccines. In this study, we determined the immunogenicity of melcam mim1 and melcam mim2, peptide mimics of an epitope of the melanoma cell-adhesion molecule (Mel-CAM). BALB/c mice were vaccinated either with mimotopes or mimotopes coupled to tetanus ... More
Evidence of GATA-3-dependent Th2 commitment during the in vivo immune response.
AuthorsTamauchi H, Terashima M, Ito M, Maruyama H, Ikewaki N, Inoue M, Gao X, Hozumi K, Habu S
JournalInt Immunol
PubMed ID14688073
The transcription factor GATA-3 has been shown to play an important role for the in vitro induction of T(h)2 cells. To clarify how the in vivo immune response is governed under GATA-3 function, we generated double-transgenic mice by crossing GATA-3 transgenic mice with ovalbumin (OVA)-specific TCR transgenic mice. After immunization ... More
The immunomodulating properties of human respiratory syncytial virus and immunostimulating complexes containing Quillaja saponin components QH-A, QH-C and ISCOPREP703.
AuthorsHu KF, Regner M, Siegrist CA, Lambert P, Chen M, Bengtsson KL, Morein B
JournalFEMS Immunol Med Microbiol
PubMed ID15681158
A successful vaccine against human RSV (HRSV) is likely to induce a Th1 or a balanced Th1/TH2 cytokine response. We tested a panel of HRSV immunostimulating complexes (ISCOMs) containing different Quillaja saponin fractions (QH-A, QH-C, and 703: a mixture of 70% QH-A and 30% QH-C) with different immunological properties for ... More
Th2-driven, allergen-induced airway inflammation is reduced after treatment with anti-Tim-3 antibody in vivo.
AuthorsKearley J, McMillan SJ, Lloyd CM
JournalJ Exp Med
PubMed ID17517968
T cell immunoglobulin and mucin domain-containing molecule-3 (Tim-3) is a surface molecule that is preferentially expressed on activated Th1 cells in comparison to Th2 cells. Blockade of Tim-3 has been shown to enhance Th1-driven pathology in vivo, suggesting that blockade of Tim-3 may improve the development of Th2-associated responses such ... More
Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice.
AuthorsJustice JP, Borchers MT, Lee JJ, Rowan WH, Shibata Y, Van Scott MR
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID11792635
Allergen-induced recruitment of T lymphocytes and eosinophils to the airways is associated with increased expression of the transcription factor GATA-3. In this study, the relationship between airway inflammation and GATA-3 expression in the lungs was investigated using ragweed-sensitized C57BL/6J mice. Intratracheal ragweed challenge increased both the number of GATA-3-expressing cells ... More
Time course of inflammatory and remodeling events in a murine model of asthma: effect of steroid treatment.
AuthorsTrifilieff A, El-Hashim A, Bertrand C
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID11076802
The kinetics of airway inflammation and remodeling processes following ovalbumin aerosol challenge in sensitized BALB/c mice was studied. Mice were exposed to either single or five ovalbumin challenges over 5 days. In both protocols, time-dependent increases in bronchoalveolar lavage (BAL) cellular fibronectin, neutrophils and eosinophils were observed. The kinetics of ... More
Effect of a single oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin on immune function in male NC/Nga mice.
AuthorsFujimaki H, Nohara K, Kobayashi T, Suzuki K, Eguchi-Kasai K, Tsukumo S, Kijima M, Tohyama C
JournalToxicol Sci
PubMed ID11861978
Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces immunosuppression in humans and animals. However, the effect of TCDD on Th2-type immune responses such as allergic reactions has been unclear. Using NC/Nga mice that developed atopic dermatitis-like skin lesions with marked elevation in plasma of total IgE when bred under conventional conditions, we investigated ... More
Cryptic determinants and promiscuous sequences on human acetylcholine receptor: HLA-dependent dichotomy in T-cell function.
AuthorsRaju R, Marietta E, Vinasco J, Conti-Fine BM, Infante AJ, David CS
JournalHum Immunol
PubMed ID12039405
Experimental autoimmune myasthenia gravis can be induced in some strains of mice and rats by immunizing with acetylcholine receptor. Also, epidemiologic studies demonstrate an MHC linkage of myasthenia gravis in the man. In order to obtain direct experimental evidence for the influence of the genes of the MHC complex in ... More
Abrogation of lung inflammation in sensitized Stat6-deficient mice is dependent on the allergen inhalation procedure.
AuthorsTrifilieff A, El-Hasim A, Corteling R, Owen CE
JournalBr J Pharmacol
PubMed ID10928961
Conflicting results have been reported about the role of Stat6 in allergen-induced airway inflammation. We have studied the influence of the allergen inhalation procedure on the inflammatory response using wild-type and Stat6-deficient mice generated on a C57BL/6 background. Animals were immunized i.p. on day 0 and 7 with ovalbumin (OVA) ... More
Interleukin-5 reduces the expression of uteroglobin-related protein (UGRP) 1 gene in allergic airway inflammation.
AuthorsChiba Y, Srisodsai A, Supavilai P, Kimura S
JournalImmunol Lett
PubMed ID15626484
Airway inflammation is thought to play a major role in the pathogenesis of bronchial asthma. The precise role of individual inflammatory cells, mediator and asthma related genes in allergic lung diseases is not completely understood. The uteroglobin-related protein (UGRP) 1 was proposed to be an asthma candidate gene and play ... More
Disruption of L-histidine decarboxylase reduces airway eosinophilia but not hyperresponsiveness.
AuthorsKoarai A, Ichinose M, Ishigaki-Suzuki S, Yamagata S, Sugiura H, Sakurai E, Makabe-Kobayashi Y, Kuramasu A, Watanabe T, Shirato K, Hattori T, Ohtsu H
JournalAm J Respir Crit Care Med
PubMed ID12480609
Histamine has a variety of airway actions and is considered to be an important mediator in asthma. This study examined the role of endogenous histamine in allergic airway eosinophil recruitment and hyperresponsiveness using L-histidine decarboxylase gene knockout mice. Histamine levels of the airways in L-histidine decarboxylase knockout mice were largely ... More
Transient anti-CD154-mediated immunotherapy of ongoing relapsing experimental autoimmune encephalomyelitis induces long-term inhibition of disease relapses.
AuthorsHoward LM, Dal Canto MC, Miller SD
JournalJ Neuroimmunol
PubMed ID12161021
Relapsing experimental autoimmune encephalomyelitis (R-EAE) is a Th1-mediated central nervous system (CNS) autoimmune disease with pathology similar to that of relapsing-remitting multiple sclerosis. Among recent therapeutic approaches to prevent or treat relapsing disease is the strategic blockade of the CD154-CD40 ligand pair interactions. We have previously shown that CD154 blockade ... More
Is IL12 necessary in experimental hypersensitivity pneumonitis?
AuthorsSchuyler M, Gott K, Cherne A
JournalInt J Exp Pathol
PubMed ID12084045
Inhalation of Saccharopolyspora rectivirgula (SR) can cause the disease Farmer's Lung, a classic example of hypersensitivity pneumonitis. Th1, but not Th2, cell lines can adoptively transfer experimental hypersensitivity pneumonitis (EHP). Substantial amounts of IL12 appear in bronchoalveolar lavage fluid (BALF) after a single intratracheal (IT) injection of SR, and SR-induced ... More
Innate and cognate mechanisms of pulmonary eosinophilia in helminth infection.
AuthorsCulley FJ, Brown A, Girod N, Pritchard DI, Williams TJ
JournalEur J Immunol
PubMed ID11981825
Passage of helminth larvae through the lungs can cause pulmonary eosinophilia that may have evolved as a means of parasite attrition. If allergic responses represent a misdirected activation of this arm of the immune system, then mechanisms governing eosinophil recruitment during infection would be expected to be closely related to ... More
Induction of mucosal tolerance with recombinant Hev b 1 and recombinant Hev b 3 for prevention of latex allergy in BALB/c mice.
AuthorsHufnagl K, Wagner B, Winkler B, Baier K, Hochreiter R, Thalhamer J, Kraft D, Scheiner O, Breiteneder H, Wiedermann U
JournalClin Exp Immunol
PubMed ID12869021
The prevalence of type I allergy to Hevea brasiliensis latex is particularly high among individuals with frequent exposure to latex products, such as health-care workers (HCW) and patients with spina bifida (SB). Treatment of latex allergy seems problematic as preventive measures, such as allergen avoidance, are not always possible and ... More
Cytokine responses in mice infected with Clonorchis sinensis.
AuthorsChoi YK, Yoon BI, Won YS, Lee CH, Hyun BH, Kim HC, Oh GT, Kim DY
JournalParasitol Res
PubMed ID12898229
FVB and BALB/c mice show different morbidity, development of Clonorchis sinensis, and pathological changes following C. sinensis infection. FVB mice are susceptible and BALB/c mice are relatively more resistant to C. sinensis infection. To investigate the relationship between cytokine reaction and susceptibility to C. sinensis infection in FVB and BALB/c ... More
Persistent secretion of IL-18 in the skin contributes to IgE response in mice.
AuthorsNakano H, Tsutsui H, Terada M, Yasuda K, Matsui K, Yumikura-Futatsugi S, Yamanaka K, Mizutani H, Yamamura T, Nakanishi K
JournalInt Immunol
PubMed ID12697661
After exposure of the skin to microbes, the host develops skin-specific inflammation and an acquired immune response, in which keratinocytes (KC) and Langerhans cells play critical roles respectively. We established two animal models. (i) We examined the importance of KC-derived IL-18 for the systemic IgE response by using a skin ... More
Cysteinyl leukotrienes regulate dendritic cell functions in a murine model of asthma.
AuthorsMachida I, Matsuse H, Kondo Y, Kawano T, Saeki S, Tomari S, Obase Y, Fukushima C, Kohno S
JournalJ Immunol
PubMed ID14734767
Dendritic cells (DCs) act as APCs in the airway and play a critical role in allergy. Cysteinyl leukotrienes (cysLTs) synthesized from arachidonic acid are primary mediators of immediate asthmatic reaction. The aim of this study was to investigate the effects of cysLTs on Dermatophagoides farinae (Der f)-pulsed mouse myeloid DCs ... More
Dietary nucleotides modulate antigen-specific type 1 and type 2 t-cell responses in young c57bl/6 mice.
AuthorsJyonouchi H, Sun S, Abiru T, Winship T, Kuchan MJ
JournalNutrition
PubMed ID10869901
Mice fed a nucleotide-free (NF) diet have impaired antibody (Ab) responses. The mechanisms responsible for this effect are not understood but may be related to specific changes in T-cell functions. The objective of this study was to examine the effects of dietary nucleotides on serum immunoglobulin-G (IgG) subclass Ab levels ... More
Allergen-specific immunosuppression by mucosal treatment with recombinant Ves v 5, a major allergen of Vespula vulgaris venom, in a murine model of wasp venom allergy.
AuthorsWinkler B, Bolwig C, Seppälä U, Spangfort MD, Ebner C, Wiedermann U
JournalImmunology
PubMed ID14632666
Up to 5% of the population suffer from systemic, 19% from local allergic hypersensitivity reactions to stinging insects. Even though specific immunotherapy is very effective in treating allergy to insect venom, new concepts of treatment strategies with only the disease eliciting allergen in recombinant form, along with antigen application via ... More
Influence of the route of sensitization on local and systemic immune responses in a murine model of type I allergy.
AuthorsRepa A, Wild C, Hufnagl K, Winkler B, Bohle B, Pollak A, Wiedermann U
JournalClin Exp Immunol
PubMed ID15196238
The pathophysiological and immunological characteristics of allergic immune responses are controlled by a variety of factors. We have studied the extent to which the route of sensitization influences allergen-specific IgE synthesis and local airway inflammation using a mouse model of allergic sensitization to the major birch pollen allergen Bet v ... More
Asian sand dust enhances ovalbumin-induced eosinophil recruitment in the alveoli and airway of mice.
AuthorsHiyoshi K, Ichinose T, Sadakane K, Takano H, Nishikawa M, Mori I, Yanagisawa R, Yoshida S, Kumagai Y, Tomura S, Shibamoto T
JournalEnviron Res
PubMed ID16307978
Asian sand dust (ASD) containing sulfate (SO4(2-)) reportedly causes adverse respiratory health effects but there is no experimental study showing the effect of ASD toward allergic respiratory diseases. The effects of ASD and ASD plus SO4(2-) toward allergic lung inflammation induced by ovalbumin (OVA) were investigated in this study. ICR ... More
DNA vaccination with map1 gene followed by protein boost augments protection against challenge with Cowdria ruminantium, the agent of heartwater.
AuthorsNyika A, Barbet AF, Burridge MJ, Mahan SM
JournalVaccine
PubMed ID11803084
A DNA vaccine encoding the immunodominant MAP1 protein of Cowdria ruminantium (Crystal Springs (CS) strain) was shown to partially protect DBA/2 mice against homologous lethal challenge. To enhance the protective capacity of this DNA vaccine, the effects of length of interval between vaccinations and of prime-boost regimes were investigated. Increasing ... More
Dietary ribonucleotides modulate type 1 and type 2 T-helper cell responses against ovalbumin in young BALB/cJ mice.
AuthorsJyonouchi H, Sun S, Winship T, Kuchan MJ
JournalJ Nutr
PubMed ID11285320
Dietary ribonucleotides have been shown to augment type 1 T-helper cell (Th1) responses to a protein antigen (Ag) in Th1-prone C57BL/6 mice, but their effects on type 2 Th (Th2)-prone mice are unknown. BALB/cJ mice have skewed Th2 responses against ovalbumin (OVA), characterized by augmented production of Th2 cytokines and ... More
Dense bodies of human cytomegalovirus induce both humoral and cellular immune responses in the absence of viral gene expression.
AuthorsPepperl S, Münster J, Mach M, Harris JR, Plachter B
JournalJ Virol
PubMed ID10846097
Infection of fibroblast cell cultures with human cytomegalovirus (HCMV) leads to the production of significant amounts of defective enveloped particles, termed dense bodies (DB). These noninfectious structures contain major antigenic determinants which are responsible for induction of both the humoral and the cellular immune response against HCMV. We tested the ... More