IL-5 ELISA Kit, Mouse

Catalog number: EMIL5

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Assay IL-5 (mouse interleukin-5) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse IL-5 ELISA Kit.

Features of the Mouse IL-5 ELISA Kit:

Target: natural and recombinant mouse interleukin-5 (IL-5)
Format: colorimetric ELISA (TMB substrate) in a clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 20 to 320 pg/mL
Sensitivity: < 5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum
Total Assay Time: 3.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with with mouse IL-2, IL-3, IL-4, IL-6, IFNγ, GM-CSF, TNFα, rat IL-5, rabbit IL-5 or human IL-5.

This mouse IL-5 ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-5 (IL-5) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains a pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Mouse
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols