Decreased erythrocyte CD44 and CD58 expression link e-waste Pb toxicity to changes in erythrocyte immunity in preschool children.
AuthorsHuo X, Dai Y, Yang T, Zhang Y, Li M, Xu X
JournalSci Total Environ
PubMed ID30763849
'Lead (Pb) toxicity damages blood cells and disturbs the immune micro-environment. When Pb enters the circulatory system, >95% of Pb accumulates in erythrocytes. We therefore conducted this study to explore the long-term effect of Pb exposure on expression of erythrocyte adhesion molecules (CD44 and CD58) and related downstream cytokine concentrations. ... More
Cardiovascular endothelial inflammation by chronic coexposure to lead (Pb) and polycyclic aromatic hydrocarbons from preschool children in an e-waste recycling area.
AuthorsZheng X, Huo X, Zhang Y, Wang Q, Zhang Y, Xu X
JournalEnviron Pollut
PubMed ID30597391
'Lead (Pb) and polycyclic aromatic hydrocarbon (PAH) exposure is positively associated with cardiovascular disease (CVD), and the possible potential mechanism may be caused by damage to the endothelium by modulation of inflammatory processes. No comprehensive research shows co-exposure of Pb and PAH on cardiovascular endothelial inflammation in electronic waste (e-waste) ... More
HIV-associated disruption of lung cytokine networks is incompletely restored in asymptomatic HIV-infected Malawian adults on antiretroviral therapy.
AuthorsJambo KC, Tembo DL, Kamng'ona AW, Musicha P, Banda DH, Kankwatira AM, Malamba RD, Allain TJ, Heyderman RS, Russell DG, Mwandumba HC
JournalERJ Open Res
PubMed ID29255717
'Disruption of lung cytokine networks during chronic HIV infection is incompletely restored in individuals on antiretroviral therapy.'
Adaptation of influenza A (H7N9) virus in primary human airway epithelial cells.
AuthorsHuang DT, Lu CY, Chi YH, Li WL, Chang LY, Lai MJ, Chen JS, Hsu WM, Huang LM
JournalSci Rep
PubMed ID28900138
'Influenza A (H7N9) is an emerging zoonotic pathogen with pandemic potential. To understand its adaptation capability, we examined the genetic changes and cellular responses following serial infections of A (H7N9) in primary human airway epithelial cells (hAECs). After 35 serial passages, six amino acid mutations were found, i.e. HA (R54G, ... More
Niche matters: The comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus.
AuthorsKhatun M, Sorjamaa A, Kangasniemi M, Sutinen M, Salo T, Liakka A, Lehenkari P, Tapanainen JS, Vuolteenaho O, Chen JC, Lehtonen S, Piltonen TT
JournalPLoS One
PubMed ID28419140
'Intrinsic inflammatory characteristics play a pivotal role in stem cell recruitment and homing through migration where the subsequent change in niche has been shown to alter these characteristics. The bone marrow mesenchymal stem cells (bmMSCs) have been demonstrated to migrate to the endometrium contributing to the stem cell reservoir and ... More
Regulatory (FoxP3
AuthorsKoh J, Hur JY, Lee KY, Kim MS, Heo JY, Ku BM, Sun JM, Lee SH, Ahn JS, Park K, Ahn MJ
JournalSci Rep
PubMed ID33149213
'Antitumor immune responses induced by immune checkpoint inhibitors anti-PD-1 or anti-PD-L1 have been used as therapeutic strategies in advanced non-small cell lung cancer (NSCLC) patients over the last decade. Favorable antitumor activity to immune checkpoint inhibitors is correlated with high PD-L1 expression, increased tumor-infiltrating lymphocytes, and decreased suppressive immune cells ... More
Elevated lead levels and adverse effects on natural killer cells in children from an electronic waste recycling area.
AuthorsZhang Y, Huo X, Cao J, Yang T, Xu L, Xu X
JournalEnviron Pollut
PubMed ID26895538
Lead (Pb) has been proved to exert immunotoxicity to influence immune homeostasis in humans. To monitor the internal Pb level and evaluate its effect on natural killer (NK) cells and cytokine/chemokine concentrations, we recruited 285 preschool children from Guiyu, one of the largest electronic waste (e-waste) destinations and recycling areas ... More
Association between serum soluble CD14 and IL-8 levels and clinical outcome in primary biliary cholangitis.
AuthorsUmemura T, Sekiguchi T, Joshita S, Yamazaki T, Fujimori N, Shibata S, Ichikawa Y, Komatsu M, Matsumoto A, Shums Z, Norman GL, Tanaka E, Ota M
JournalLiver Int
PubMed ID27860118
Primary biliary cholangitis (PBC) is an autoimmune liver disease characterized by portal inflammation and immune-mediated destruction of intrahepatic bile ducts that often leads to liver decompensation and liver failure. Although the biochemical response to ursodeoxycholic acid (UDCA) can predict disease outcome in PBC, few biomarkers have been identified as prognostic ... More
Human IDO-competent, long-lived immunoregulatory dendritic cells induced by intracellular pathogen, and their fate in humanized mice.
Targeting of myeloid-dendritic cell receptor DC-SIGN by numerous chronic infectious agents, including Porphyromonas gingivalis, is shown to drive-differentiation of monocytes into dysfunctional mDCs. These mDCs exhibit alterations of their fine-tuned homeostatic function and contribute to dysregulated immune-responses. Here, we utilize P. gingivalis mutant strains to show that pathogen-differentiated mDCs from ... More
Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474.
AuthorsFazekas J, Grunt TW, Jensen-Jarolim E, Singer J
JournalOncotarget
PubMed ID28402269
Cancer cell lines are indispensible surrogate models in cancer research, as they can be used off-the-shelf, expanded to the desired extent, easily modified and exchanged between research groups for affirmation, reproduction or follow-up experiments.As malignant cells are prone to genomic instability, phenotypical changes may occur after certain passages in culture. ... More
Excess a-synuclein compromises phagocytosis in iPSC-derived macrophages.
AuthorsHaenseler W, Zambon F, Lee H, Vowles J, Rinaldi F, Duggal G, Houlden H, Gwinn K, Wray S, Luk KC, Wade-Martins R, James WS, Cowley SA
JournalSci Rep
PubMed ID28827786
To examine the pathogenic role of a-synuclein (aS) in Parkinson's Disease, we have generated induced Pluripotent Stem Cell lines from early onset Parkinson's Disease patients with SNCA A53T and SNCA Triplication mutations, and in this study have differentiated them to PSC-macrophages (pMac), which recapitulate many features of their brain-resident cousins, ... More
Heparin-gold nanoparticles for enhanced microdialysis sampling.
Cerebral microdialysis is a sampling technique which offers much potential for understanding inflammatory pathophysiology following traumatic brain injury (TBI). At present, the recovery of cytokines via microdialysis in clinical studies is not straightforward primarily due to their size, steric properties and low concentrations. Heparin and heparin-coated microspheres have previously shown ... More
Cross-laboratory evaluation of multiplex bead assays including independent common reference standards for immunological monitoring of observational and interventional human studies.
Authorsvan Meijgaarden KE, Khatri B, Smith SG, Drittij AMFH, de Paus RA, Goeman JJ, Ho MM, Dockrell HM, McShane H, Joosten SA, Ottenhoff THM
JournalPLoS One
PubMed ID30180167
Multiplex assays are increasingly applied to analyze multicomponent signatures of human immune responses, including the dynamics of cytokine and chemokine production, in observational as well as interventional studies following treatment or vaccination. However, relatively limited information is available on the performance of the different available multiplex kits, and comparative evaluations ... More
IL-1ß induces expression of costimulatory molecules and cytokines but not immune feedback regulators in dendritic cells.
AuthorsMichelini S, Sarajlic M, Duschl A, Horejs-Hoeck J
JournalHum Immunol
PubMed ID29886260
Dendritic cells play an important role in the initiation of immune reactions. Due to their high capacity to prime T-cell responses, the activation of dendritic cells must be tightly controlled. Because Interleukin-1ß (IL-1ß) is a key player in autoinflammatory diseases, we compared the ability of IL-1ß to activate human dendritic ... More
Elevated inflammatory Lp-PLA2 and IL-6 link e-waste Pb toxicity to cardiovascular risk factors in preschool children.
AuthorsLu X, Xu X, Zhang Y, Zhang Y, Wang C, Huo X
JournalEnviron Pollut
PubMed ID29223817
Cardiovascular toxicity of lead (Pb) manifests primarily as an effect on blood pressure and eventual increased risk of atherosclerosis and cardiovascular events. Therefore, we investigated vascular inflammatory biomarkers and cardiovascular effects of Pb-exposed children. A total of 590 children (3-7 years old) were recruited from Guiyu (n = 337), an electronic waste ... More
Increased memory T cell populations in Pb-exposed children from an e-waste-recycling area.
AuthorsCao J, Xu X, Zhang Y, Zeng Z, Hylkema MN, Huo X
JournalSci Total Environ
PubMed ID29096958
Chronic exposure to heavy metals could affect cell-mediated immunity. The aim of this study was to explore the status of memory T cell development in preschool children from an e-waste recycling area. Blood lead (Pb) levels, peripheral T cell subpopulations, and serum levels of cytokines (IL-2/IL-7/IL-15), relevant to generation and ... More
Biallelic interferon regulatory factor 8 mutation: A complex immunodeficiency syndrome with dendritic cell deficiency, monocytopenia, and immune dysregulation.
AuthorsBigley V, Maisuria S, Cytlak U, Jardine L, Care MA, Green K, Gunawan M, Milne P, Dickinson R, Wiscombe S, Parry D, Doffinger R, Laurence A, Fonseca C, Stoevesandt O, Gennery A, Cant A, Tooze R, Simpson AJ, Hambleton S, Savic S, Doody G, Collin M
JournalJ Allergy Clin Immunol
PubMed ID29128673
The homozygous K108E mutation of interferon regulatory factor 8 (IRF8) is reported to cause dendritic cell (DC) and monocyte deficiency. However, more widespread immune dysfunction is predicted from the multiple roles ascribed to IRF8 in immune cell development and function. ... More
Functional characterization and phenotypic monitoring of human hematopoietic stem cell expansion and differentiation of monocytes and macrophages by whole-cell mass spectrometry.
AuthorsVogel G, Cuénod A, Mouchet R, Strauss A, Daubenberger C, Pflüger V, Portevin D
JournalStem Cell Res
PubMed ID29227832
The different facets of macrophages allow them to play distinct roles in tissue homeostasis, tissue repair and in response to infections. Individuals displaying dysregulated macrophage functions are proposed to be prone to inflammatory disorders or infections. However, this being a cause or a consequence of the pathology remains often unclear. ... More
Coactivation of TLR2 and TLR8 in Primary Human Monocytes Triggers a Distinct Inflammatory Signaling Response.
AuthorsBösl K, Giambelluca M, Haug M, Bugge M, Espevik T, Kandasamy RK, Bergstrøm B,
JournalFront Physiol
PubMed ID29896111
Innate immune signaling is essential to mount a fast and specific immune response to pathogens. Monocytes and macrophages are essential cells in the early response in their capacity as ubiquitous phagocytic cells. They phagocytose microorganisms or damaged cells and sense pathogen/damage-associated molecular patterns (PAMPs/DAMPs) through innate receptors such as Toll-like ... More
Dampening of CD8+ T Cell Response by B Cell Depletion Therapy in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
AuthorsNéel A, Bucchia M, Néel M, Tilly G, Caristan A, Yap M, Rimbert M, Bruneau S, Cadoux M, Agard C, Hourmant M, Godmer P, Brouard S, Bressollette C, Hamidou M, Josien R, Fakhouri F, Degauque N
JournalArthritis Rheumatol
PubMed ID30375745
To compare the effects of rituximab (RTX) and conventional immunosuppressants (CIs) on CD4+ T cells, Treg cells, and CD8+ T cells in antineutrophil cytoplasmic antibody-associated vasculitis (AAV). ... More
Soluble ST2 and CD163 as Potential Biomarkers to Differentiate Primary Hemophagocytic Lymphohistiocytosis from Macrophage Activation Syndrome.
AuthorsGao Z, Wang Y, Wang J, Zhang J, Wang Z
JournalMediterr J Hematol Infect Dis
PubMed ID30671214
The differentiation of primary hemophagocytic lymphohistiocytosis (pHLH) and macrophage activation syndrome (MAS) poses a challenge to hematologists. The aim of this study was (1) to compare the levels of soluble ST2 (sST2), sCD163, IFN-?, IL-10, IL-18, TNF-a and Serum soluble interleukin-2 receptor (sCD25) in patients with pHLH and MAS and ... More