Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection.
AuthorsTay MZ, Rouers A, Fong SW, Goh YS, Chan YH, Chang ZW, Xu W, Tan CW, Chia WN, Torres-Ruesta A, Amrun SN, Huang Y, Hor PX, Loh CY, Yeo NK, Wang B, Ngoh EZX, Salleh SNM, Chavatte JM, Lim AJ, Maurer-Stroh S, Wang LF, Lin RVTP, Wang CI, Tan SY, Young BE, Leo YS, Lye DC, Renia L, Ng LF
JournalEMBO Mol Med
PubMed ID34994081
The SARS-CoV-2 Delta (B.1.617.2) variant is capable of infecting vaccinated persons. An open question remains as to whether deficiencies in specific vaccine-elicited immune responses result in susceptibility to vaccine breakthrough infection. We investigated 55 vaccine breakthrough infection cases (mostly Delta) in Singapore, comparing them against 86 vaccinated close contacts who ... More
Effects of altered salt intake and diet on cytokines in humans: A 20-week randomized cross-over intervention study.
AuthorsNiiranen T,Erlund I,Jalkanen S,Jula A,Salmi M
JournalEuropean journal of immunology
PubMed ID36330564
Zika Virus Infection Preferentially Counterbalances Human Peripheral Monocyte and/or NK Cell Activity.
AuthorsLum FM, Lee D, Chua TK, Tan JJL, Lee CYP, Liu X, Fang Y, Lee B, Yee WX, Rickett NY, Chia PY, Lim V, Leo YS, Matthews DA, Hiscox JA, Ng LFP
Journal
PubMed ID29600283
'Zika virus (ZIKV) has reemerged in the population and caused unprecedented global outbreaks. Here, the transcriptomic consequences of ZIKV infection were studied systematically first in human peripheral blood CD14' ... More
Influenza Virus Infection Enhances Antibody-Mediated NK Cell Functions via Type I Interferon-Dependent Pathways.
AuthorsJegaskanda S, Vanderven HA, Tan HX, Alcantara S, Wragg KM, Parsons MS, Chung AW, Juno JA, Kent SJ
JournalJ Virol
PubMed ID30541850
'Natural killer (NK) cells are an important component in the control of influenza virus infection, acting to both clear virus-infected cells and release antiviral cytokines. Engagement of CD16 on NK cells by antibody-coated influenza virus-infected cells results in antibody-dependent cellular cytotoxicity (ADCC). Increasing the potency of antibody-mediated NK cell activity ... More
Deciphering the state of immune silence in fatal COVID-19 patients.
AuthorsBost P, De Sanctis F, Canè S, Ugel S, Donadello K, Castellucci M, Eyal D, Fiore A, Anselmi C, Barouni RM, Trovato R, Caligola S, Lamolinara A, Iezzi M, Facciotti F, Mazzariol A, Gibellini D, De Nardo P, Tacconelli E, Gottin L, Polati E, Schwikowski B, Amit I, Bronte V
JournalNat Commun
PubMed ID33674591
'Since the beginning of the SARS-CoV-2 pandemic, COVID-19 appeared as a unique disease with unconventional tissue and systemic immune features. Here we show a COVID-19 immune signature associated with severity by integrating single-cell RNA-seq analysis from blood samples and broncho-alveolar lavage fluids with clinical, immunological and functional ex vivo data. This ... More
IL-10, IL-13, Eotaxin and IL-10/IL-6 ratio distinguish breast implant-associated anaplastic large-cell lymphoma from all types of benign late seromas.
AuthorsDi Napoli A, Greco D, Scafetta G, Ascenzi F, Gulino A, Aurisicchio L, Santanelli Di Pompeo F, Bonifacino A, Giarnieri E, Morgan J, Mancini R, Kadin ME
JournalCancer Immunol Immunother
PubMed ID33146828
'Breast implant-associated anaplastic large-cell lymphoma (BI-ALCL) is an uncommon peripheral T cell lymphoma usually presenting as a delayed peri-implant effusion. Chronic inflammation elicited by the implant has been implicated in its pathogenesis. Infection or implant rupture may also be responsible for late seromas. Cytomorphological examination coupled with CD30 immunostaining and ... More
Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses.
AuthorsRitter M, Ndongmo WPC, Njouendou AJ, Nghochuzie NN, Nchang LC, Tayong DB, Arndts K, Nausch N, Jacobsen M, Wanji S, Layland LE, Hoerauf A
JournalPLoS Negl Trop Dis
PubMed ID29324739
'The filarial nematode Mansonella perstans is endemic throughout Africa, northern South America and the Caribbean. Interestingly, M. perstans-infected individuals present no distinct clinical picture associated with certain pathology. Due to its relatively silent nature, research on this tropical disease has been neglected, especially M. perstans-driven immune responses. A hindrance in ... More
Differential and Overlapping Immune Programs Regulated by IRF3 and IRF5 in Plasmacytoid Dendritic Cells.
AuthorsChow KT, Wilkins C, Narita M, Green R, Knoll M, Loo YM, Gale M
JournalJ Immunol
PubMed ID30297339
'We examined the signaling pathways and cell type-specific responses of IFN regulatory factor (IRF) 5, an immune-regulatory transcription factor. We show that the protein kinases IKKa, IKKß, IKKe, and TANK-binding kinase 1 each confer IRF5 phosphorylation/dimerization, thus extending the family of IRF5 activator kinases. Among primary human immune cell subsets, ... More
Assessment of biomarkers using multiplex assays in aqueous humor of patients with diabetic retinopathy.
AuthorsChen H, Zhang X, Liao N, Wen F
JournalBMC Ophthalmol
PubMed ID28969616
With the high prevalence of type 2 diabetes, diabetic retinopathy (DR) has become a leading health problem worldwide. The pathogenesis of DR is complex and several vascular, inflammatory, and neuronal mechanisms are involved. The purpose of this study was to assess the levels of immune and inflammatory biomarkers in the ... More
Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells.
Worldwide, more than 1 billion people suffer from allergic diseases. However, until now it is not fully understood how certain proteins can induce allergic immune responses, while others cannot. Studies suggest that allergenicity is a process not only determined by properties of the allergen itself but also by costimulatory factors, ... More
Subcutaneous and Visceral Adipose Tissue Secretions from Extremely Obese Men and Women both Acutely Suppress Muscle Insulin Signaling.
AuthorsSarr O, Strohm RJ, MacDonald TL, Gaudio N, Reed JK, Foute-Nelong J, Dyck DJ, Mutch DM
JournalInt J Mol Sci
PubMed ID28468326
Adipose tissue plays a key role in the development of type-2 diabetes via the secretion of adipokines. The current study investigated if secretion media derived from intact visceral (VAT) and subcutaneous (SAT) adipose tissues from extremely obese men and women differently suppressed insulin signaling in human skeletal myotubes derived from ... More
Human Adipose-Derived Mesenchymal Stem Cells Respond to Short-Term Hypoxia by Secreting Factors Beneficial for Human Islets In Vitro and Potentiate Antidiabetic Effect In Vivo.
AuthorsSchive SW, Mirlashari MR, Hasvold G, Wang M, Josefsen D, Gullestad HP, Korsgren O, Foss A, Kvalheim G, Scholz H
JournalCell Med
PubMed ID28713640
Adipose-derived mesenchymal stem cells (ASCs) release factors beneficial for islets in vitro and protect against hyperglycemia in rodent models of diabetes. Oxygen tension has been shown to induce metabolic changes and alter ASCs' release of soluble factors. The effects of hypoxia on the antidiabetic properties of ASCs have not been ... More
Elevation of CD16
AuthorsWang J, Han W, Gao Z, Wang Y, Wu L, Zhang J, Lai W, Wang Z
JournalHematology
PubMed ID28413901
Encouraging progress has been made in application of splenectomy in the treatment of relapsed hemophagocytic lymphohistiocytosis (HLH) of unknown cause. The aim was to determine the roles of lymphocyte subpopulations and inflammatory cytokines in splenectomy. ... More
Cytokines in umbilical cord blood-derived cellular product: a mechanistic insight into bone repair.
AuthorsSane MS, Misra N, Mousa OM, Czop S, Tang H, Khoo LT, Jones CD, Mustafi SB
JournalRegen Med
PubMed ID30346891
Umbilical cord blood (UCB) finds frequent applications in regenerative medicine. We evaluated the role of cytokines present in a uniquely processed, UCB-derived cellular allograft product (UCBp). ... More
Cultivated Human Vaginal Microbiome Communities Impact Zika and Herpes Simplex Virus Replication in
AuthorsAmerson-Brown MH, Miller AL, Maxwell CA, White MM, Vincent KL, Bourne N, Pyles RB
JournalFront Microbiol
PubMed ID30692980
The human vaginal microbiome (VMB) is a complex bacterial community that interacts closely with vaginal epithelial cells (VECs) impacting the mucosal phenotype and its responses to pathogenic insults. The VMB and VEC relationship includes nutrient exchange and regulation of signaling molecules that controls numerous host functions and defends against invading ... More
Longitudinal Study of Cellular and Systemic Cytokine Signatures to Define the Dynamics of a Balanced Immune Environment During Disease Manifestation in Zika Virus-Infected Patients.
AuthorsLum FM, Lye DCB, Tan JJL, Lee B, Chia PY, Chua TK, Amrun SN, Kam YW, Yee WX, Ling WP, Lim VWX, Pang VJX, Lee LK, Mok EWH, Chong CY, Leo YS, Ng LFP
JournalJ Infect Dis
PubMed ID29672707
Since its unexpected reemergence, Zika virus (ZIKV) has caused numerous outbreaks globally. This study characterized the host immune responses during ZIKV infection.
A Preclinical Model for the ATLL Lymphoma Subtype With Insights Into the Role of Microenvironment in HTLV-1-Mediated Lymphomagenesis.
AuthorsVicario M, Mattiolo A, Montini B, Piano MA, Cavallari I, Amadori A, Chieco-Bianchi L, Calabrò ML
JournalFront Microbiol
PubMed ID29951044
Adult T cell Leukemia/Lymphoma (ATLL) is a mature T cell malignancy associated with Human T cell Leukemia Virus type 1 (HTLV-1) infection. Among its four main clinical subtypes, the prognosis of acute and lymphoma variants remains poor. The long latency (3-6 decades) and low incidence (3-5%) of ATLL imply the ... More
Monocyte-Derived Signals Activate Human Natural Killer Cells in Response to
Activated natural killer (NK) cells release interferon (IFN)-?, which is crucial for the control of intracellular pathogens such as
Improving hematopoietic recovery through modeling and modulation of the mesenchymal stromal cell secretome.
AuthorsLiu FD, Tam K, Pishesha N, Poon Z, Van Vliet KJ
JournalStem Cell Res Ther
PubMed ID30352620
Efficient and sustained hematopoietic recovery after hematopoietic stem cell or bone marrow transplantation is supported by paracrine signaling from specific subpopulations of mesenchymal stromal cells (MSCs). Here, we considered whether in vitro mechanopriming of human MSCs could be administered to predictively and significantly improve in vivo hematopoietic recovery after irradiation ... More
Multiple antigen-engineered DC vaccines with or without IFNa to promote antitumor immunity in melanoma.
AuthorsButterfield LH, Vujanovic L, Santos PM, Maurer DM, Gambotto A, Lohr J, Li C, Waldman J, Chandran U, Lin Y, Lin H, Tawbi HA, Tarhini AA, Kirkwood JM
JournalJ Immunother Cancer
PubMed ID31014399
Cancer vaccines are designed to promote systemic antitumor immunity and tumor eradication. Cancer vaccination may be more efficacious in combination with additional interventions that may build on or amplify their effects. ... More
Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems.
The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the ... More
Changes in aqueous oxidative stress, prostaglandins, and cytokines: Comparisons of low-energy femtosecond laser-assisted cataract surgery versus conventional phacoemulsification.
AuthorsLiu YC, Setiawan M, Ang M, Yam GHF, Mehta JS
JournalJ Cataract Refract Surg
PubMed ID30528516
To compare the aqueous oxidative stress, prostaglandin E
Effects of a major deletion in the SARS-CoV-2 genome on the severity of infection and the inflammatory response: an observational cohort study.
AuthorsYoung BE, Fong SW, Chan YH, Mak TM, Ang LW, Anderson DE, Lee CY, Amrun SN, Lee B, Goh YS, Su YCF, Wei WE, Kalimuddin S, Chai LYA, Pada S, Tan SY, Sun L, Parthasarathy P, Chen YYC, Barkham T, Lin RTP, Maurer-Stroh S, Leo YS, Wang LF, Renia L, Lee VJ, Smith GJD, Lye DC, Ng LFP
JournalLancet
PubMed ID32822564
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with a 382-nucleotide deletion (?382) in the open reading frame 8 (ORF8) region of the genome have been detected in Singapore and other countries. We investigated the effect of this deletion on the clinical features of infection. ... More
Dynamics of SARS-CoV-2 neutralising antibody responses and duration of immunity: a longitudinal study.
AuthorsChia WN, Zhu F, Ong SWX, Young BE, Fong SW, Le Bert N, Tan CW, Tiu C, Zhang J, Tan SY, Pada S, Chan YH, Tham CYL, Kunasegaran K, Chen MI, Low JGH, Leo YS, Renia L, Bertoletti A, Ng LFP, Lye DC, Wang LF
JournalLancet Microbe
PubMed ID33778792
Studies have found different waning rates of neutralising antibodies compared with binding antibodies against SARS-CoV-2. The impact of neutralising antibody waning rate at the individual patient level on the longevity of immunity remains unknown. We aimed to investigate the peak levels and dynamics of neutralising antibody waning and IgG avidity ... More
Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection.
AuthorsTay MZ, Rouers A, Fong SW, Goh YS, Chan YH, Chang ZW, Xu W, Tan CW, Chia WN, Torres-Ruesta A, Amrun SN, Huang Y, Hor PX, Loh CY, Yeo NK, Wang B, Ngoh EZX, Salleh SNM, Chavatte JM, Lim AJ, Maurer-Stroh S, Wang LF, Lin RVTP, Wang CI, Tan SY, Young BE, Leo YS, Lye DC, Renia L, Ng LF
JournalEMBO Mol Med
PubMed IDs
The SARS-CoV-2 Delta (B.1.617.2) variant is capable of infecting vaccinated persons. An open question remains as to whether deficiencies in specific vaccine-elicited immune responses result in susceptibility to vaccine breakthrough infection. We investigated 55 vaccine breakthrough infection cases (mostly Delta) in Singapore, comparing them against 86 vaccinated close contacts who ... More