Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ Panel 1

Catalog number: EPX450-12171-901

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

This kit does not contain a Multiscreen Filter Plate.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
BDNF; Eotaxin/CCL11; EGF; FGF-2; GM-CSF; GRO alpha/CXCL1; HGF; NGF beta; LIF; IFN alpha; IFN gamma; IL-1 beta; IL-1 alpha; IL-1RA; IL-2; IL-4; IL-5; IL-6; IL-7; IL-8/CXCL8; IL-9; IL-10; IL-12 p70; IL-13; IL-15; IL-17A; IL-18; IL-21; IL-22; IL-23; IL-27; IL-31; IP-10/CXCL10; MCP-1/CCL2; MIP-1 alpha/CCL3; MIP-1 beta/CCL4; RANTES/CCL5; SDF-1 alpha/CXCL12; TNF alpha; TNF beta/LTA; PDGF-BB; PLGF; SCF; VEGF-A; VEGF-D

Bead Regions
IFN gamma: 43; IL-12p70: 34 ; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF alpha: 45; GM-CSF: 44; IL-18: 66; IL-10: 28; IL-17A: 36; IL-21: 72; IL-22: 76; IL-23: 63; IL-27: 14; IL-9: 52; IFN alpha: 48; IL-31: 37; IL-15: 65; IL-1 alpha: 62; IL-1RA: 38; IL-7: 26; TNF beta: 54; Eotaxin: 33; GRO alpha: 61; IL-8: 27; IP-10: 22; MCP-1: 51; MIP-1 alpha: 12; MIP-1 beta: 47; RANTES: 42; SDF-1 alpha: 13; NGF beta: 55; EGF: 56; FGF-2: 75; HGF: 46; BDNF: 57; PDGF-BB: 77; PIGF-1: 29; SCF: 39; VEGF-A: 78; LIF: 15; VEGF-D: 53

Assay Range
See product documentation

Assay Sensitivity
See product documentation

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Former Identifiers: eBioscience
Detection Method: Fluorescent
For Use With (Equipment): Luminex™ Instruments
Immunoassay Kit Format: Panel
Interassay CV: Less than 10%
Intraassay CV: Less than 10%
Label or Dye: R-PE
Multiplex Panel Name: Cytokine/Chemokine/Growth Factor
Multiplex Panel Version: 1
Multiplex Size (Plex No.): 45
Product Line: ProcartaPlex™
Product Size: 96 tests
Sample Type (Specific): Cell Culture Supernatants, Plasma, Serum
Sample Volume: CCS: 50 µL, Serum, Plasma: 25 µL
System: Luminex™
Target Species (Named): Human
Target Species (Validated): Human

Contents & storage

2 vials Human Standard Mix A
2 vials Human Standard Mix B
2 vials Human Standard Mix C
1 vial Human Bead Mix B
1 vial Human Bead Mix C
1 vial Human Bead Mix D
1 vial Human Bead Mix F
1 vial Human Bead Mix G
1 vial Human Detection Antibody Mix B
1 vial Human Detection Antibody Mix C
1 vial Human Detection Antibody Mix D
1 vial Human Detection Antibody Mix E
1 vial Human Detection Antibody Mix G
1 bottle Reading Buffer
1 bottle Wash Buffer 10x
1 bottle Streptavidin-PE
1 bottle Universal Assay Buffer
1 bottle Detection Antibody Diluent
PCR 8-Tube Strip
Adhesive Film
Greiner Microplate black 96 well

Store at 2-8°C.

Documents

Manuals & protocols