CRP Rat ELISA Kit

Catalog number: ERCRP

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Rat C-reactive protein ELISA research-use-only kit is an enzyme-linked immunosorbent assay designed for the quantitation and detection of C-reactive protein (CRP) in samples (see sample types indicated) using a microplate reader. The assay will recognize both natural and recombinant forms of this target.

This complete, ready-to-use kit has been developed and optimized to specifically detect C-reactive protein (CRP) and includes antibody pre-coated plate(s) and other components to perform the assay. Using the supplied pre-coated, pre-calibrated plate helps reduce variability issues associated with using other kits and assays that require you to coat plates with consistent levels of antibody.

Performance characteristics

• Sensitivity: 200 pg/mL
• Standard curve range: 0.2 ng/mL–60 ng/mL
• Sample type(s): cell culture supernatants, plasma, serum
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
• Sample volume: 100 µL per sample after dilution
• Total assay time: 5 hours

Rigorous validation

To help ensure sensitive, accurate, and consistent performance, this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, lot-to-lot consistency, recovery, linearity, and parallelism. See product inserts for further information on validation for this kit.

Learn more about our rigorous ELISA validation and quality testing process.

Principle of the method

This C-reactive protein (CRP) solid-phase, sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure and detect the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation periods and wash steps to remove unbound antibody from the plate, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is proportional to the concentration of target present in the original specimen.

Related links

Learn more about ELISA kits.
Learn more about other immunoassays.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Proteases Peptidases & Inhibitors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: PTX1
Gene ID: 25419
Gene Name: C-reactive protein
Gene Symbol: CRP
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: C-reactive protein
Shipping Condition: Approved for shipment on Wet or Dry Ice
Target (Kit Named): CRP
Target Species (Named): Rat
Target Species (Validated): Rat
UniProt ID: P48199

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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