ICAM-1 Rat ELISA Kit

Catalog number: ERICAM1

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Rat ICAM-1 ELISA research-use-only kit is an enzyme-linked immunosorbent assay designed for the quantitation and detection of ICAM-1 in samples (see sample types indicated) using a microplate reader. The assay will recognize both natural and recombinant forms of this target.

This complete, ready-to-use kit has been developed and optimized to specifically detect ICAM-1 and includes antibody pre-coated plate(s) and other components to perform the assay. Using the supplied pre-coated, pre-calibrated plate helps reduce variability issues associated with using other kits and assays that require you to coat plates with consistent levels of antibody.

Performance characteristics

• Sensitivity: 20 pg/ml
• Standard curve range: 20 pg/ml - 7000 pg/ml
• Sample type(s): cell culture supernatants, plasma, serum
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
• Sample volume: 100 µL per sample after dilution
• Total assay time: 5 hours

Rigorous validation

To help ensure sensitive, accurate, and consistent performance, this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, lot-to-lot consistency, recovery, linearity, and parallelism. See product inserts for further information on validation for this kit.

Learn more about our rigorous ELISA validation and quality testing process.

Principle of the method

This ICAM-1 solid-phase, sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure and detect the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation periods and wash steps to remove unbound antibody from the plate, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is proportional to the concentration of target present in the original specimen.

Related links

Learn more about ELISA kits.
Learn more about other immunoassays.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Chemokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: CD54, ICAM
Gene ID: 25464
Gene Name: intercellular adhesion molecule 1
Gene Symbol: ICAM1
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: ICAM-1
Protein Name: intercellular adhesion molecule 1
Shipping Condition: Approved for shipment on Wet or Dry Ice
Target (Kit Named): ICAM-1
Target Species (Named): Rat
Target Species (Validated): Rat
UniProt ID: Q00238

Contents & storage

Pre-coated 96-well plate, standard, detector antibody, streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers. May be stored at 2–8°C for up to 6 months from the date of shipment.

Documents

Manuals & protocols

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