IFN gamma Rat ELISA Kit

Catalog number:  ERIFNG

 Related applications: Protein Assays and Analysis

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Use the Thermo Scientific® Rat IFN gamma ELISA Kit to assay IFN-γ (rat interferon gamma) levels in rat serum, plasma (with EDTA, heparin, sodium citrate), and cell culture supernatant samples. This is an alternate product for Cat. No. KRC4021.

This kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of IFNγ levels in samples. The Thermo Scientific® Rat IFN gamma ELISA Kit also includes calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution, and stop solution. This complete kit contains one pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: natural and recombinant rat interferon gamma (IFNγ)
• Assay range: 8 to 500 pg/mL
• Sensitivity: <2 pg/mL
• Sample types: cell culture supernatant, serum, plasma (with EDTA, sodium citrate, heparin)
• Total assay time: 2.5 hours
• Sample Volume: 50 μL
• Specificity: no significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, or TNFα, or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250pg/mL mouse IFNγ was equivalent to 258pg/mL rat IFNγ (4.1% cross-reactivity)
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: IFNG2
Gene ID: 25712
Gene Name: interferon gamma
Gene Symbol: IFNG
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: IFN-gamma
Protein Name: Interferon gamma
Target (Kit Named): IFN gamma
Target Species (Named): Rat
Target Species (Validated): Rat
UniProt ID: P01581

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols