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IFN gamma Rat ELISA Kit

Catalog number: ERIFNG5

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay IFN-γ (rat interferon gamma) levels in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Rat IFN gamma ELISA Kits.

Features of the Rat IFN gamma ELISA Kit:

Target: natural and recombinant rat interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 8 to 500 pg/mL
Sensitivity: < 2 pg/mL
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 2.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, or TNFα, or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250 pg/mL mouse IFNγ was equivalent to 258 pg/mL rat IFNγ (4.1% cross-reactivity).

The Rat IFN gamma ELISA Kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. This complete kit contains precoated 96-well strip plates and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: IFNG2
Gene ID: 25712
Gene Name: interferon gamma
Gene Symbol: IFNG
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: IFN-gamma
Protein Name: Interferon gamma
Target (Kit Named): IFN gamma
Target Species (Named): Rat
Target Species (Validated): Rat
UniProt ID: P01581

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols