IL-1 beta Bovine Uncoated ELISA Kit

Catalog number:  ESS0027

 Related applications: Protein Assays and Analysis

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Assay IL-1β (bovine interleukin-1 beta) levels in bovine cell culture supernatant samples with our Thermo Scientific Bovine IL-1 beta ELISA Kit.

Features of the Bovine IL-1 beta ELISA Kit:

Target: natural and recombinant bovine interleukin-1 beta (IL-1β)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31 to 2000 pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3.5 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with recombinant bovine IL-2, IL-4, IL-6, IL-8, IFNγ, or TNFα.

This bovine IL-1 beta ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene ID: 281251
Gene Name: interleukin 1 beta
Gene Symbol: IL1B
Immunoassay Kit Format: Uncoated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: IL-1 beta
Protein Name: Interleukin-1 beta
Target (Kit Named): IL-1 beta
Target Species (Named): Bovine
Target Species (Validated): Bovine
UniProt ID: P09428

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols