Citations et références (13)

Invitrogen™

Kit de dosage de calcium Fluo-4 Direct™

Name: Kit de dosage de calcium Fluo-4 Direct™
SKU: F10471
Price: 553.65 EUR

Kit d’essai de calcium Fluo-4 Direct™

Modifier l'affichage

Référence	Quantité
F10471	100 ml, 100 ml
F10472	100 ml, 100 ml
F10473	1 000 ml, 1000 ml

3 options

Référence F10471

Prix (EUR)

553,65

Offre exceptionnelle en ligne

636,00

Économisez 82,35 (13%)

Each

Quantité:

100 ml, 100 ml

Prix (EUR)

553,65

Offre exceptionnelle en ligne

636,00

Économisez 82,35 (13%)

Each

Le kit de dosage du calcium Fluo-4 Direct™ a été formulé pour fournir un dosage du calcium fluorescent et homogène qui présente les caractéristiques suivantes :
1. Se charge facilement dans les cellules
2. Permet d’obtenir une grande fenêtre de dosage et
3. Supprime la fluorescence de fond générée à partir de l’indicateur de calcium dans un milieu complet avec peu ou pas d’impact sur la fluorescence cellulaire spécifique

Cette formulation avancée permet au test d’être exécuté dans un format de “simple ajout” uniquement en présence de milieux contenant du sérum. Fluo-4 Direct™ constitue la troisième nouveauté de la famille Fluo-4 de réactifs de détection du calcium. Fluo-4 AM et Fluo-4 NW nécessitent tous deux l’élimination des milieux avant la détection par dosage, mais le Fluo-4 NW améliore le confort d’utilisation en incluant le réactif PowerLoad™ afin de faciliter le chargement des cellules. Le kit de dosage du calcium Fluo-4 Direct™ est semblable au Fluo-4 NW en ce qu’il est formulé avec le réactif PowerLoad™ afin de faciliter le chargement des cellules, mais il est unique en ce qu’il peut être utilisé en présence de milieux de culture complets et qu’il supprime efficacement la fluorescence du fond sans sacrifier la fluorescence cellulaire spécifique générée lors du dosage.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Méthode de détectionFluorescence, Fluorescent

Type de colorantFluorescent à base de colorants

FormePoudre

ModificationProduits chimiques

Quantité100 ml, 100 ml

Conditions d’expéditionTempérature ambiante

Localisation subcellulaireNoyau, organites, cytoplasme

CouleurVert

EmissionVisible

À utiliser avec (application)Dosage du calcium

À utiliser avec (équipement)Microscope confocal, microscope à fluorescence, instrument à haut contenu, lecteur HTS, lecteur de microplaques, imageur fluorescent, Microscope confocal, Microscope à fluorescence, Instruments d’analyse à haut contenu, Lecteur HTS, Lecteur de microplaques, Imageur fluorescent

Gamme de produitsMolecular Probes

Type de produitFlux de calcium cellulaire

Unit SizeEach

Contenu et stockage

Réactif de dosage du calcium direct Fluo-4 de 10 x 10 l (composant A)
2 x 77 mg de probénécide (composant B)
Tampon de dosage direct de calcium Fluo-4 Direct de 200 ml (composant C)
Conserver à ≤-20°C. À déshydrater et à tenir à l’abri de la lumière.

Foire aux questions (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (13)

Citations et références

Abstract

High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.

Authors:Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ,

Journal:J R Soc Interface

PubMed ID:21490003

'T-cell activation via antigen presentation is associated with the formation of a macromolecular membrane assembly termed the immunological synapse (IS). The genesis of the IS and the onset of juxtacrine signalling is characterized by the formation of cell membrane microclusters and the organization of such into segregated microdomains. A central ... More

Regulation of cytosolic phospholipase A2 (cPLA2alpha) and its association with cell proliferation in human lens epithelial cells.

Authors:Wang Y, Xing KY, Lou MF,

Journal:Invest Ophthalmol Vis Sci

PubMed ID:21896865

'To investigate the molecular mechanism for cytosolic phospholipase A2 (cPLA(2)a) regulation and its association to platelet-derived growth factor (PDGF)-induced cell proliferation. cPLA(2)a was examined using human lens epithelial (HLE) B3 cells. Reactive oxygen species (ROS) generation induced by PDGF was analyzed by luminescence assay. Cell proliferation was measured by cell ... More

20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.

Authors:Wen H, Östman J, Bubb KJ, Panayiotou C, Priestley JV, Baker MD, Ahluwalia A,

Journal:J Biol Chem

PubMed ID:22389490

'TRPV1 is a member of the transient receptor potential ion channel family and is gated by capsaicin, the pungent component of chili pepper. It is expressed predominantly in small diameter peripheral nerve fibers and is activated by noxious temperatures >42 °C. 20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P-450 4A/4F-derived metabolite ... More

Gq/5-HT2c receptor signals activate a local GABAergic inhibitory feedback circuit to modulate serotonergic firing and anxiety in mice.

Authors:Spoida K, Masseck OA, Deneris ES, Herlitze S,

Journal:

PubMed ID:24733892

Serotonin 2c receptors (5-HT2c-Rs) are drug targets for certain mental disorders, including schizophrenia, depression, and anxiety. 5-HT2c-Rs are expressed throughout the brain, making it difficult to link behavioral changes to circuit specific receptor expression. Various 5-HT-Rs, including 5-HT2c-Rs, are found in the dorsal raphe nucleus (DRN); however, the function of ... More

Small molecule inhibition of PAX3-FOXO1 through AKT activation suppresses malignant phenotypes of alveolar rhabdomyosarcoma.

Authors:Jothi M, Mal M, Keller C, Mal AK,

Journal:

PubMed ID:24107448

Alveolar rhabdomyosarcoma comprises a rare highly malignant tumor presumed to be associated with skeletal muscle lineage in children. The hallmark of the majority of alveolar rhabdomyosarcoma is a chromosomal translocation that generates the PAX3-FOXO1 fusion protein, which is an oncogenic transcription factor responsible for the development of the malignant phenotype ... More

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