Search

  • Statut des commandes
  • Commande rapide
  • Promos
Product Image
Citations et références (8)
Invitrogen™

Kit de dosage de calcium Fluo-4 Direct™

Kit d’essai de calcium Fluo-4 Direct™
Have Questions?
Modifier l'affichagebuttonViewtableView
RéférenceQuantité
F10472100 ml, 100 ml
F10471100 ml, 100 ml
F104731 000 ml, 1000 ml
Référence F10472
Prix (EUR)
514,65
Offre exceptionnelle en ligne
592,00
Économisez 77,35 (13%)
Each
Quantité:
100 ml, 100 ml
Prix (EUR)
514,65
Offre exceptionnelle en ligne
592,00
Économisez 77,35 (13%)
Each
Le kit de dosage du calcium Fluo-4 Direct™ a été formulé pour fournir un dosage du calcium fluorescent et homogène qui présente les caractéristiques suivantes :
1. Se charge facilement dans les cellules
2. Permet d’obtenir une grande fenêtre de dosage et
3. Supprime la fluorescence de fond générée à partir de l’indicateur de calcium dans un milieu complet avec peu ou pas d’impact sur la fluorescence cellulaire spécifique

Cette formulation avancée permet au test d’être exécuté dans un format de “simple ajout” uniquement en présence de milieux contenant du sérum. Fluo-4 Direct™ constitue la troisième nouveauté de la famille Fluo-4 de réactifs de détection du calcium. Fluo-4 AM et Fluo-4 NW nécessitent tous deux l’élimination des milieux avant la détection par dosage, mais le Fluo-4 NW améliore le confort d’utilisation en incluant le réactif PowerLoad™ afin de faciliter le chargement des cellules. Le kit de dosage du calcium Fluo-4 Direct™ est semblable au Fluo-4 NW en ce qu’il est formulé avec le réactif PowerLoad™ afin de faciliter le chargement des cellules, mais il est unique en ce qu’il peut être utilisé en présence de milieux de culture complets et qu’il supprime efficacement la fluorescence du fond sans sacrifier la fluorescence cellulaire spécifique générée lors du dosage.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Méthode de détectionFluorescence, Fluorescent
Type de colorantFluorescent à base de colorants
FormePoudre
ModificationProduits chimiques
Quantité100 ml, 100 ml
Conditions d’expéditionTempérature ambiante
Localisation subcellulaireNoyau, organites, cytoplasme
CouleurVert
EmissionVisible
À utiliser avec (application)Dosage du calcium
À utiliser avec (équipement)Microscope confocal, microscope à fluorescence, instrument à haut contenu, lecteur HTS, lecteur de microplaques, imageur fluorescent, Microscope confocal, Microscope à fluorescence, Instruments d’analyse à haut contenu, Lecteur HTS, Lecteur de microplaques, Imageur fluorescent
Gamme de produitsMolecular Probes
Type de produitKit de dosage du calcium
Unit SizeEach
Contenu et stockage
  • Réactif de dosage direct du calcium Fluo-4 de 100 ml (composant A)
  • 2 x 77 mg de probénécide (composant B)
  • Tampon de dosage direct de calcium Fluo-4 Direct de 200 ml (composant C)
  • Conserver à ≤-20°C. À déshydrater et à tenir à l’abri de la lumière.

Foire aux questions (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (8)

Citations et références
Abstract
High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.
Authors:Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ,
Journal:J R Soc Interface
PubMed ID:21490003
'T-cell activation via antigen presentation is associated with the formation of a macromolecular membrane assembly termed the immunological synapse (IS). The genesis of the IS and the onset of juxtacrine signalling is characterized by the formation of cell membrane microclusters and the organization of such into segregated microdomains. A central ... More
Transient receptor potential vanilloid-1 (TRPV1) is a mediator of lung toxicity for coal fly ash particulate material.
Authors:Deering-Rice CE, Johansen ME, Roberts JK, Thomas KC, Romero EG, Lee J, Yost GS, Veranth JM, Reilly CA,
Journal:Mol Pharmacol
PubMed ID:22155782
'Environmental particulate matter (PM) pollutants adversely affect human health, but the molecular basis is poorly understood. The ion channel transient receptor potential vanilloid-1 (TRPV1) has been implicated as a sensor for environmental PM and a mediator of adverse events in the respiratory tract. The objectives of this study were to ... More
20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.
Authors:Wen H, Östman J, Bubb KJ, Panayiotou C, Priestley JV, Baker MD, Ahluwalia A,
Journal:J Biol Chem
PubMed ID:22389490
'TRPV1 is a member of the transient receptor potential ion channel family and is gated by capsaicin, the pungent component of chili pepper. It is expressed predominantly in small diameter peripheral nerve fibers and is activated by noxious temperatures >42 °C. 20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P-450 4A/4F-derived metabolite ... More
Screening ß-arrestin recruitment for the identification of natural ligands for orphan G-protein-coupled receptors.
Authors:Southern C, Cook JM, Neetoo-Isseljee Z, Taylor DL, Kettleborough CA, Merritt A, Bassoni DL, Raab WJ, Quinn E, Wehrman TS, Davenport AP, Brown AJ, Green A, Wigglesworth MJ, Rees S,
Journal:J Biomol Screen
PubMed ID:23396314
A variety of G-protein-coupled receptor (GPCR) screening technologies have successfully partnered a number of GPCRs with their cognate ligands. GPCR-mediated ß-arrestin recruitment is now recognized as a distinct intracellular signaling pathway, and ligand-receptor interactions may show a bias toward ß-arrestin over classical GPCR signaling pathways. We hypothesized that the failure ... More
A novel in vitro model system for smooth muscle differentiation from human embryonic stem cell-derived mesenchymal cells.
Authors:Guo X, Stice SL, Boyd NL, Chen SY,
Journal:Am J Physiol Cell Physiol
PubMed ID:23220114
The objective of this study was to develop a novel in vitro model for smooth muscle cell (SMC) differentiation from human embryonic stem cell-derived mesenchymal cells (hES-MCs). We found that hES-MCs were differentiated to SMCs by transforming growth factor-ß (TGF-ß) in a dose- and time-dependent manner as demonstrated by the ... More
View all Kit de dosage de calcium Fluo-4 Direct™ citations