Thermo Scientific™

Phire Hot Start II PCR Master Mix

Catalog number: F125L
Thermo Scientific™

Phire Hot Start II PCR Master Mix

Catalog number: F125L
Catalog Number
F125L
also known as F-125L
Unit Size
1,000 reactions
Price (USD)
Price: 1,036.00
Your Price:
Availability
***
Quantity
Catalog Number
F125S
also known as F-125S
Unit Size
200 reactions
Price (USD)
Price: 263.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F125L
also known as F-125L
1,000 reactions
Price: 1,036.00
Your Price:
***
F125S
also known as F-125S
200 reactions
Price: 263.00
Your Price:
***
Product Overview
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Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction.

Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

Highlights

Quick hot start—No reactivation step
Fast enzyme—Amplify four times faster than with hot start Taq
Robust—Minimal reaction optimization due to high inhibitor tolerance
High yields—Abundant products due to high efficiency
Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq

Applications

• Hot Start PCR
• Routine PCR
• Non-high fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
PCR Amplification
Concentration
2X
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
Medium
Hot Start
Built-In Hot Start
No. of Reactions
1000 Reactions
Overhang
Blunt
Polymerase
Phire Hot Start II DNA polymerase
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
Starting Material
DNA
Includes
2X Phire Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO
Product Line
Phire
Product Type
Hot Start PCR Master Mix
Quantity
1000 x 50 μL Reactions

Contents & Storage

• 20 x 1.25 mL 2X Phire Hot Start II PCR Master Mix
• 4 x 0.5 mL DMSO
• 20 x 1.25 mL Water, nuclease free

Store at -20°C.

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