Invitrogen™

Fluo-4, Pentapotassium Salt, cell impermeant

Référence: F14200
Invitrogen™

Fluo-4, Pentapotassium Salt, cell impermeant

Référence: F14200
Référence
F14200
Taille de l’unité
500 μg
Prix (EUR)
Prix 367,00
Votre prix
Disponibilité
***
Quantité
RéférenceTaille de l’unitéPrix (EUR)DisponibilitéQuantité
F14200500 μg
Prix 367,00
Votre prix
***
Présentation du produit
Figures
Vidéos
Documentation
Foire aux questions
Citations et références
Informations supplémentaires
Recommandations
Recommandations
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette, microinjection, or our Influx™ pinocytotic cell-loading reagent. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Calcium Indicator (Cell-Impermeant Salts) Specifications:
• Label (Ex/Em of Ca2+–bound form): Fluo-4 (494/516 nm)
• Fluorescence intensity increase upon binding Ca2+: >100 fold
• Kd for Ca2+ in buffer: ∼335 nM
• Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength


Using TPEN to Control Heavy Metal Cations
In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.

More Choices for Fluorescent Calcium Indicators
We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) review Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Spécifications

Méthode de détection
Fluorescent
À utiliser avec (équipement)
Microscope confocal, Microscope à fluorescence, Instrument à grande capacité, Lecteur HTS, Lecteur de microplaques, Imageur fluorescent
Type de produit
Indicateur de calcium
Type de colorant
Fluorescent à base de colorants
Conditions d’expédition
Température ambiante

Contenu et stockage

Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.