Can I use Fluo-4, Pentapotassium Salt, cell impermeant (Cat. No. F14200) in low pH conditions (pH 4-5)?
No. pH affects the ability of Fluo-4, Pentapotassium Salt, cell impermeant (Cat. No. F14200) to bind calcium and the fluorescence intensity. The Fluo-4 dye drops in fluorescence below pH 5.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.