LIM domain only 4 (LMO4) regulates calcium-induced calcium release and synaptic plasticity in the hippocampus.
AuthorsQin Z, Zhou X, Gomez-Smith M, Pandey NR, Lee KF, Lagace DC, Béïque JC, Chen HH,
JournalJ Neurosci
PubMed ID22442089
'The LIM domain only 4 (LMO4) transcription cofactor activates gene expression in neurons and regulates key aspects of network formation, but the mechanisms are poorly understood. Here, we show that LMO4 positively regulates ryanodine receptor type 2 (RyR2) expression, thereby suggesting that LMO4 regulates calcium-induced calcium release (CICR) in central ... More
nNOS(+) striatal neurons, a subpopulation spared in Huntington's Disease, possess functional NMDA receptors but fail to generate mitochondrial ROS in response to an excitotoxic challenge.
'Huntington''s disease (HD) is a neurodegenerative condition characterized by severe neuronal loss in the cortex and striatum that leads to motor and behavioral deficits. Excitotoxicity is thought to be involved in HD and several studies have indicated that NMDA receptor (NMDAR) overactivation can play a role in the selective neuronal ... More
Mechanisms underlying cannabinoid inhibition of presynaptic Ca2+ influx at parallel fibre synapses of the rat cerebellum.
AuthorsDaniel H, Rancillac A, Crepel F
JournalJ Physiol
PubMed ID15034129
'Activation of CB1 cannabinoid receptors in the cerebellum acutely depresses excitatory synaptic transmission at parallel fibre-Purkinje cell synapses by decreasing the probability of glutamate release. This depression involves the activation of presynaptic 4-aminopyridine-sensitive K(+) channels by CB1 receptors, which in turn inhibits presynaptic Ca(2+) influx controlling glutamate release at these ... More
Cross-tolerance to otherwise lethal N-methyl-D-aspartate and oxygen-glucose deprivation in preconditioned cortical cultures.
AuthorsTauskela JS, Comas T, Hewitt K, Monette R, Paris J, Hogan M, Morley P
JournalNeuroscience
PubMed ID11720781
'In vitro ischemic preconditioning induced by subjecting rat cortical cultures to nonlethal oxygen-glucose deprivation protects against a subsequent exposure to otherwise lethal oxygen-glucose deprivation. We provide evidence that attenuation of the postsynaptic N-methyl-D-aspartate (NMDA) receptor- and Ca(2+)-dependent neurotoxicity underlies oxygen-glucose deprivation tolerance. It is demonstrated that extended tolerance to otherwise ... More
Mitochondrial calcium uniporter silencing potentiates caspase-independent cell death in MDA-MB-231 breast cancer cells.
'The mitochondrial calcium uniporter (MCU) transports free ionic Ca(2+) into the mitochondrial matrix. We assessed MCU expression in clinical breast cancer samples using microarray analysis and the consequences of MCU silencing in a breast cancer cell line. Our results indicate that estrogen receptor negative and basal-like breast cancers are characterized ... More
Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators.
AuthorsHofer AM,
JournalMethods Mol Biol
PubMed ID16422202
This chapter provides a guide for the use of compartmentalized fluorescentdyes to measure free [Ca2+] in the agonist-sensitive internal store of single cells. The basic steps involve incubating cells with the AM-ester derivative of the appropriate dye (resulting in partitioning of the probe in both the cytoplasm and organelles), and ... More
Inositol 1,4,5-triphosphate drives glutamatergic and cholinergic inhibition selectively in spiny projection neurons in the striatum.
AuthorsClements MA, Swapna I, Morikawa H,
JournalJ Neurosci
PubMed ID23392696
The striatum is critically involved in the selection of appropriate actions in a constantly changing environment. The spiking activity of striatal spiny projection neurons (SPNs), driven by extrinsic glutamatergic inputs, is shaped by local GABAergic and cholinergic networks. For example, it is well established that different types of GABAergic interneurons, ... More
Fluorescent measurement of [Ca²?]c: basic practical considerations.
AuthorsSimpson AW,
JournalMethods Mol Biol
PubMed ID23007577
There is a vast array of dyes currently available for measurement of cytosolic calcium. These encompass single and dual excitation and single and dual emission probes. The choice of particular probe depends on the experimental question and the type of equipment to be used. It is therefore extremely difficult to ... More
Concurrent maturation of inner hair cell synaptic Ca2+ influx and auditory nerve spontaneous activity around hearing onset in mice.
AuthorsWong AB, Jing Z, Rutherford MA, Frank T, Strenzke N, Moser T,
Journal
PubMed ID23804089
Hearing over a wide range of sound intensities is thought to require complementary coding by functionally diverse spiral ganglion neurons (SGNs), each changing activity only over a subrange. The foundations of SGN diversity are not well understood but likely include differences among their inputs: the presynaptic active zones (AZs) of ... More
Selective permeability of different connexin channels to the second messenger cyclic AMP.
AuthorsBedner P, Niessen H, Odermatt B, Kretz M, Willecke K, Harz H
JournalJ Biol Chem
PubMed ID16373337
Gap junctions are intercellular conduits that are formed in vertebrates by connexin proteins and allow diffusion exchange of intracellular ions and small molecules. At least 20 different connexin genes in the human and mouse genome are cell-type specifically expressed with overlapping expression patterns. A possible explanation for this diversity could ... More
Na+-dependent inactivation of the retinal cone/brain Na+/Ca2+-K+ exchanger NCKX2.
AuthorsAltimimi HF, Schnetkamp PP
JournalJ Biol Chem
PubMed ID17164249
The SLC24 gene family Na+/Ca2+-K+ exchangers (NCKX) are bidirectional plasma membrane transporters whose main function is the extrusion of Ca2+ from the cytosol. In this study, we used human embryonic kidney 293 cells expressing human retinal cone/brain NCKX2 to examine its Na+ affinity and kinetic parameters of Ca2+ transport. With ... More
Rapid suppression of mitochondrial permeability transition by methylglyoxal. Role of reversible arginine modification.
AuthorsSpeer O, Morkunaite-Haimi S, Liobikas J, Franck M, Hensbo L, Linder MD, Kinnunen PK, Wallimann T, Eriksson O
JournalJ Biol Chem
PubMed ID12815061
Methylglyoxal (MG) (pyruvaldehyde) is a reactive carbonyl compound produced in glycolysis. MG can form covalent adducts on proteins resulting in advanced glycation end products that may alter protein function. Here we report that MG covalently modifies the mitochondrial permeability transition pore (PTP), a high conductance channel involved in the signal ... More
Control of Ca(2+) influx by cannabinoid and metabotropic glutamate receptors in rat cerebellar cortex requires K(+) channels.
AuthorsDaniel H, Crepel F
JournalJ Physiol
PubMed ID11744755
1. In the rodent cerebellum, both presynaptic CB1 cannabinoid receptors and presynaptic mGluR4 metabotropic glutamate receptors acutely depress excitatory synaptic transmission at parallel fibre-Purkinje cell synapses. Using rat cerebellar slices, we have analysed the effects of selective CB1 and mGluR4 agonists on the presynaptic Ca(2+) influx which controls glutamate release ... More
Heterogeneity of Ca2+-permeable AMPA/kainate channel expression in hippocampal pyramidal neurons: fluorescence imaging and immunocytochemical assessment.
AuthorsOgoshi F, Weiss JH
JournalJ Neurosci
PubMed ID14627636
The presence of Ca2+-permeable AMPA/kainate (Ca-A/K) channels on hippocampal pyramidal neurons (HPNs) has been controversial, although they are present on many forebrain GABAergic neurons. We combined high-resolution fluorescence Ca2+ imaging with surface AMPA receptor (AMPAR) subunit immunocytochemistry to examine the expression of functional Ca-A/K channels in dissociated hippocampal neurons at ... More
Effects of electrical shocks on Cai2+ and Vm in myocyte cultures.
AuthorsFast VG, Cheek ER, Pollard AE, Ideker RE
JournalCirc Res
PubMed ID15155528
Changes in intracellular calcium concentration (DeltaCa(i)2+) induced by electrical shocks may play an important role in defibrillation, but high-resolution DeltaCa(i)2+ measurements in a multicellular cardiac tissue and their relationship to corresponding Vm changes (DeltaVm) are lacking. Here, we measured shock-induced DeltaCa(i)2+ and DeltaV(m) in geometrically defined myocyte cultures. Cell strands ... More
Spontaneous Calcium Oscillations through Differentiation: A Calcium Imaging Analysis of Rat Cochlear Nucleus Neural Stem Cells.
Authors
JournalCells
PubMed ID34685782
Mitochondrial Dysfunction Associates With Acute T Lymphocytopenia and Impaired Functionality in COVID-19 Patients.