Uncoupling of the base excision and nucleotide incision repair pathways reveals their respective biological roles.
AuthorsIshchenko AA, Deprez E, Maksimenko A, Brochon JC, Tauc P, Saparbaev MK
JournalProc Natl Acad Sci U S A
PubMed ID16473948
'The multifunctional DNA repair enzymes apurinic/apyrimidinic (AP) endonucleases cleave DNA at AP sites and 3''-blocking moieties generated by DNA glycosylases in the base excision repair pathway. Alternatively, in the nucleotide incision repair (NIR) pathway, the same AP endonucleases incise DNA 5'' of a number of oxidatively damaged bases. At present, ... More
Fluorescence imaging study of extracellular zinc at the hippocampal mossy fiber synapse.
AuthorsBastian C, Li YV
JournalNeurosci Lett
PubMed ID17485170
'Although synaptically released, vesicular Zn(2+) has been proposed to play a neuromodulatory or neuronal signaling role at the mossy fiber-CA3 synapse, Zn(2+) release remains controversial, especially when detected using fluorescent imaging. In the present study, we investigated synaptically released Zn(2+) at the mossy fiber (MF) synapse in rat hippocampal slices ... More
Determining zinc with commonly used calcium and zinc fluorescent indicators, a question on calcium signals.
AuthorsMartin JL, Stork CJ, Li YV
JournalCell Calcium
PubMed ID16764924
'Investigations into the roles of Ca(2+) and Zn(2+) in cell biology have been facilitated by the development of sensitive fluorometric probes that have enabled the measurement of Ca(2+) or Zn(2+) in both extracellular and intracellular environments. It is critical to be aware of the specificity and relative selectivity of a ... More
Integration of multianalyte sensing functions on a capillary-assembled microchip: simultaneous determination of ion concentrations and enzymatic activities by a "drop-and-sip" technique.
AuthorsHenares TG, Takaishi M, Yoshida N, Terabe S, Mizutani F, Sekizawa R, Hisamoto H
JournalAnal Chem
PubMed ID17263315
'A general and simple implementation of simultaneous multiparametric sensing in a single microchip is presented by using a capillary-assembled microchip (CAs-CHIP) integrated with the plural different reagent-release capillaries (RRCs), acting as various biochemical sensors. A novel "drop-and-sip" technique of fluid handling is performed with a microliter droplet of a model ... More
Dual nanomolar and picomolar Zn(II) binding properties of metallothionein.
AuthorsKrezel A, Maret W,
JournalJ Am Chem Soc
PubMed ID17696343
'Each of the seven Zn(II) ions in the Zn(3)S(9) and Zn(4)S(11) clusters of human metallothionein is in a tetrathiolate coordination environment. Yet analysis of Zn(II) association with thionein, the apoprotein, and analysis of Zn(II) dissociation from metallothionein using the fluorescent chelating agents FluoZin-3 and RhodZin-3 reveal at least three classes ... More
Measuring cell viability with membrane impermeable zinc fluorescent indicator.
AuthorsStork CJ, Li YV,
JournalJ Neurosci Methods
PubMed ID16466804
'Recent findings suggest that the accumulation of cytoplasmic zinc [Zn2+]i is a ubiquitous component in the cell death cascade. Zn2+ can be liberated from intracellular stores following oxidative stress and contribute to cell death processes. Here we show that the membrane/cell impermeable Zn2+ fluorescent indicator Newport Green (NG), which is ... More
Metals in neurobiology: probing their chemistry and biology with molecular imaging.
AuthorsQue EL, Domaille DW, Chang CJ,
JournalChem Rev
PubMed ID18426241
'In this review, we will provide a brief overview of the field of transition metals in neurobiology, focusing on the contributions of d-block metals zinc, iron, and copper to neurophysiology, aging, and neuropathology, as well as progress in the development of molecular probes for visualizing zinc, iron, and copper ion ... More
Biomimetic nanostructures: creating a high-affinity zinc-binding site in a folded nonbiological polymer.
AuthorsLee BC, Chu TK, Dill KA, Zuckermann RN,
JournalJ Am Chem Soc
PubMed ID18597438
'One of the long-term goals in developing advanced biomaterials is to generate protein-like nanostructures and functions from a completely nonnatural polymer. Toward that end, we introduced a high-affinity zinc-binding function into a peptoid (N-substituted glycine polymer) two-helix bundle. Borrowing from well-understood zinc-binding motifs in proteins, thiol and imidazole moieties were ... More
Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction.
AuthorsHao M, Head WS, Gunawardana SC, Hasty AH, Piston DW,
JournalDiabetes
PubMed ID17575085
'OBJECTIVE: Type 2 diabetes is often accompanied by abnormal blood lipid and lipoprotein levels, but most studies on the link between hyperlipidemia and diabetes have focused on free fatty acids (FFAs). In this study, we examined the relationship between cholesterol and insulin secretion from pancreatic beta-cells that is independent of ... More
Simultaneous detection of intracellular free calcium and zinc using fura-2FF and FluoZin-3.
AuthorsDevinney MJ, Reynolds IJ, Dineley KE
JournalCell Calcium
PubMed ID15670869
'Elevation of intracellular free zinc ([Zn2+]i) probably contributes to cell death in injury paradigms involving calcium deregulation and oxidative stress such as glutamate excitotoxicity. However, it is difficult to monitor both ions simultaneously in live cells. Here we present a new method using fluorescence microscopy and the ion sensitive indicators ... More
Visualization of transmitter release with zinc fluorescence detection at the mouse hippocampal mossy fibre synapse.
AuthorsQian J, Noebels JL
JournalJ Physiol
PubMed ID15919713
'Exocytosis of synaptic vesicle contents defines the quantal nature of neurotransmitter release. Here we developed a technique to directly assess exocytosis by measuring vesicular zinc release with the zinc-sensitive dye FluoZin-3 at the hippocampal mossy fibre (MF) synapse. Using a photodiode, we were able to clearly resolve the zinc fluorescence ... More
Detection and imaging of zinc secretion from pancreatic beta-cells using a new fluorescent zinc indicator.
AuthorsGee KR, Zhou ZL, Qian WJ, Kennedy R
JournalJ Am Chem Soc
PubMed ID11817952
The zinc indicator FluoZin-3 is not perturbed significantly by physiological levels of calcium or magnesium.
AuthorsZhao J, Bertoglio BA, Gee KR, Kay AR,
JournalCell Calcium
PubMed ID18353435
There has been some dispute in the literature as to the sensitivity of the zinc indicator FluoZin-3 to calcium, with suggestions that physiological levels of calcium and magnesium effectively occlude the response of the probe to zinc. In this communication we demonstrate that calcium concentrations as high as 10mM do ... More
The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green.
AuthorsZhao J, Bertoglio BA, Devinney MJ, Dineley KE, Kay AR,
JournalAnal Biochem
PubMed ID18848515
Zinc-sensitive fluorescent probes have become increasingly important in the investigation of the cellular roles of zinc. There is, however, little information on how the other transition metals in cells may influence the measurement of zinc. We have characterized in vitro the interaction of the nominal zinc indicators FluoZin-3 and Newport ... More
Synthetic fluorescent sensors for studying the cell biology of metals.
AuthorsDomaille DW, Que EL, Chang CJ,
JournalNat Chem Biol
PubMed ID18277978
Metals are essential for sustaining all forms of life, but alterations in their cellular homeostasis are connected to severe human disorders, including cancer, diabetes and neurodegenerative diseases. Fluorescent small molecules that respond to metal ions in the cell with appropriate selectivity and sensitivity offer the ability to probe physiological and ... More
Detecting and minimizing zinc contamination in physiological solutions.
AuthorsKay AR
JournalBMC Physiol
PubMed ID15113426
BACKGROUND: To explore the role of zinc (Zn) in cellular physiology it is important to be able to control and quantify the level of Zn contamination in experimental solutions. A technique that relies on a Zn-sensitive fluorimetric probe is introduced for measuring Zn concentrations as low as 100 pM. The ... More
Critical role of transmembrane segment zinc binding in the structure and function of rhodopsin.
AuthorsStojanovic A, Stitham J, Hwa J
JournalJ Biol Chem
PubMed ID15194703
Zinc deficiency and retinitis pigmentosa are both important factors resulting in retinal dysfunction and night blindness. In this study, we address the critical biochemical and structural relevance of zinc ions in rhodopsin and examine whether zinc deficiency can lead to rhodopsin dysfunction. We report the identification of a high-affinity zinc ... More
Simultaneous monitoring of Zn2+ secretion and intracellular Ca2+ from islets and islet cells by fluorescence microscopy.
A method for simultaneously imaging Zn2+ secretion and intracellular Ca2+ at beta-cell clusters and single islets of Langerhans was developed. Cells were loaded with the Ca2+ indicator Fura Red, incubated in buffer containing the Zn2+ indicator FluoZin-3, and imaged via laser scanning fluorescence confocal microscopy. FluoZin-3 and Fura Red are ... More
Exocytosis of vesicular zinc reveals persistent depression of neurotransmitter release during metabotropic glutamate receptor long-term depression at the hippocampal CA3-CA1 synapse.
AuthorsQian J, Noebels JL
JournalJ Neurosci
PubMed ID16738253
Exocytosis can be directly measured in mammalian brain slices by fluorescence detection of vesicular zinc release. Detection of the low-level evoked zinc signal [Zn]t was first demonstrated at the zinc-rich hippocampal mossy fiber pathway and required the use of high-frequency presynaptic stimulation. Here, we show that release after individual action ... More
Zinc-buffering capacity of a eukaryotic cell at physiological pZn.
AuthorsKrezel A, Maret W
JournalJ Biol Inorg Chem
PubMed ID16924557
In spite of the paramount importance of zinc in biology, dynamic aspects of cellular zinc metabolism remain poorly defined at the molecular level. Investigations with human colon cancer (HT-29) cells establish a total cellular zinc concentration of 264 microM. Remarkably, about 10% of the potential high-affinity zinc-binding sites are not ... More
A novel and simple fluorescence method for the measurement of presynaptic vesicular zinc release in acute hippocampal slices with a fluorescence plate reader.
AuthorsDatki ZL, Hunya A, Penke B
JournalBrain Res Bull
PubMed ID17683806
The synaptic vesicles in the hippocampal neuronal terminals are abundantly supplied with zinc ions (Zn2+), which can be released into the synaptic cleft. In the glutamatergic systems (e.g. the hippocampus and the amygdala), the vesicular Zn2+ is co-localized with glutamate (Glu). Glu functions as a neurotransmitter, and Zn2+ as a ... More
Protocol for Quantifying Zinc Flux in Cultured Cells using Fluorescent Indicators.
Authors
JournalSTAR Protoc
PubMed ID33111096
Microtubules regulate pancreatic β-cell heterogeneity via spatiotemporal control of insulin secretion hot spots.
Authors
JournalElife
PubMed ID34783306
Novel Zinc-Attenuating Compounds as Potent Broad-Spectrum Antifungal Agents with In Vitro and In Vivo Efficacy.
Authors
JournalAntimicrob Agents Chemother
PubMed ID29439980
Determination of trace zinc in seawater by coupling solid phase extraction and fluorescence detection in the Lab-On-Valve format.
Authors
JournalAnal Chim Acta
PubMed ID27155301
Cryo-EM structures of human ZnT8 in both outward- and inward-facing conformations.
Authors
JournalElife
PubMed ID32723473
Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and ZP2 Cleavage to Prevent Polyspermy.