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FluoZin™-3, AM, cell permeant

Catalog number: F24195

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Target & Lead Identification & Validation

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Description

The FluoZin™-3 indicator is a Zn2+-selective indicator with a structure that resembles fluo-4. FluoZin™-3 exhibits high Zn2+-binding affinity that is unperturbed by Ca2+ concentrations up to at least 1 µM. In addition, FluoZin™-3 exhibits a >50-fold increase in fluorescence in response to saturating levels of Zn2+. Although imaging applications of FluoZin™-3 are predominant, cell-based microplate assays and flow cytometry protocols have also been developed. FluoZin™-3 indicator is available in a cell-permeant and a cell-impermeant form.

Fluorescent Zinc Indicators Specifications:
• Label (Ex/Em): FluoZin™-3 (~494/516 nm)
• Kd (Zn2+) (in buffer): ~15 nM
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding the dissolved indicator to medium containing cells


Find Fluorescent Indicators for Zinc and Other Metal Ions
We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more. Review Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: Zinc Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Fluorescence Microscope
Cell Permeability: Cell-Permeant
Product Size: 100 µg
Detection Method: Fluorescent
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols

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