DyNAmo Flash SYBR Green qPCR Kit
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Thermo Scientific™

DyNAmo Flash SYBR Green qPCR Kit

**********************Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at aRead more
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Catalog NumberNo. of Reactions
F415L500 reactions
F415XL2500 Reactions
Catalog number F415L
Price (USD)
626.00
Each
Add to cart
No. of Reactions:
500 reactions
Price (USD)
626.00
Each
Add to cart
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo Flash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo Flash SYBR Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantification and early Cq values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.

Highlights

• Extremely fast protocols: Combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2× master mix and optimized protocol

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNAmo Flash Probe qPCR Kit
DyNAmo Flash SYBR Green qPCR Kit
DyNAmo HS SYBR Green qPCR Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X (DyNAmo Flash SYBR Green qPCR Mix), 50X (ROX Dye)
For Use With (Equipment)7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
FormatTube
Kit TypeDyNAmo Flash SYBR Green qPCR Kit
No. of Reactions500 reactions
PolymeraseTbr DNA polymerase
Product LineDyNAmo Flash
Product TypeReal Time PCR SYBR Master Mix
Quantity500 reactions
Sample TypeDNA, dsDNA
Sufficient For500 Reactions of 20 μL, 200 Reactions of 50 μL
Volume5 x 1 mL
Detection MethodSYBR
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
Contains:
• 5 x 1 mL DyNAmo Flash SYBR Green qPCR Mix (2X)
• 1 x 250 μL 50X ROX dye, 25μM

Store at -20°C

Frequently asked questions (FAQs)

Do I need to use Fast plastics to run a Fast qPCR reaction?

Specialized Fast PCR plates are not required if you are using a standard qPCR machine with a fast protocol. However, Fast Block PCR Plates (AB-1900) can be purchased which are compatible with the thermal blocks on fast qPCR thermal cyclers.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.