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Fluorescein Phalloidin, Green, 496/516 nm - FAQs

View additional product information for Phalloidin Labeling Probes - FAQs (R415, A12379, A30104, B7474, A22282, A30105, A22281, A30106, A30107, A22286, P3457, F432, A22285, A22284, A22283, T7471, A22287, B3475, A34055, A12380, O7466, A12381, A34054)

2 product FAQs found

I want to label F-actin with phalloidin and image with transmission electron microscopy. What is the best way to go about it?

One way is to use a biotin-phalloidin conjugate, then label that with a colloidal gold conjugate of streptavidin. You will need to block endogenous biotin first. Another method is to label with fluorescein phalloidin, then with an anti-fluorescein antibody conjugated with colloidal gold.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.