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Fluorescein Phalloidin

Catalog number: F432

Molecular Probes™  Related applications: Cell Structure

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Description

Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC).

Selectively stains F-actin
Excitation/Emission: 496/516 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while fluorescein provides green fluorescence. Demonstrating very little nonspecific staining, fluorescein phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Fluorescein phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Fluorescein phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: FITC (Fluorescein)
Sub-Cellular Localization: Actin, Cytoskeleton
Excitation⁄Emission (nm): 496⁄516
Product Size: 300 units
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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