Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC).
• Selectively stains F-actin • Excitation/Emission: 496/516 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples
Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while fluorescein provides green fluorescence. Demonstrating very little nonspecific staining, fluorescein phalloidin allows high-contrast discrimination of actin staining.
Use in Multiple Applications Fluorescein phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Fluorescein phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.
Not intended for human or animal therapeutic or diagnostic use.