SOD2 functions downstream of Sch9 to extend longevity in yeast.
AuthorsFabrizio P, Liou LL, Moy VN, Diaspro A, Valentine JS, Gralla EB, Longo VD
JournalGenetics
PubMed ID12586694
'Signal transduction pathways inactivated during periods of starvation are implicated in the regulation of longevity in organisms ranging from yeast to mammals, but the mechanisms responsible for life-span extension are poorly understood. Chronological life-span extension in S. cerevisiae cyr1 and sch9 mutants is mediated by the stress-resistance proteins Msn2/Msn4 and ... More
Early events in macrophage killing of Aspergillus fumigatus conidia: new flow cytometric viability assay.
AuthorsMarr KA, Koudadoust M, Black M, Balajee SA
JournalClin Diagn Lab Immunol
PubMed ID11687470
'Detailed investigations of macrophage phagocytosis and killing of Aspergillus fumigatus conidia have been limited by technical difficulties in quantifying fungal uptake and viability. In order to study early events in cell pathogen ingestion and killing, we developed a new flow cytometry assay that utilizes the fungus-specific viability dye FUN-1. Metabolically ... More
Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation.
AuthorsHeystek HC, Moulon C, Woltman AM, Garonne P, van Kooten C
JournalJ Immunol
PubMed ID11751952
'Immature dendritic cells (DC) reside in peripheral tissues, where they pick up and process incoming pathogens via scavenger receptors or FcR such as FcgammaR and FcepsilonR. At mucosal surfaces, IgA is the main Ig to protect the body from incoming pathogens. In addition, DC are present in high numbers at ... More
In vitro growth and analysis of Candida biofilms.
AuthorsChandra J, Mukherjee PK, Ghannoum MA,
JournalNat Protoc
PubMed ID19180075
'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More
Viruses activate a genetically conserved cell death pathway in a unicellular organism.
AuthorsIvanovska I, Hardwick JM
JournalJ Cell Biol
PubMed ID16061692
'Given the importance of apoptosis in the pathogenesis of virus infections in mammals, we investigated the possibility that unicellular organisms also respond to viral pathogens by activating programmed cell death. The M1 and M2 killer viruses of Saccharomyces cerevisiae encode pore-forming toxins that were assumed to kill uninfected yeast cells ... More
Pleiotropic effects of HIV-1 protein R (Vpr) on morphogenesis and cell survival in fission yeast and antagonism by pentoxifylline.
AuthorsZhao Y, Yu M, Chen M, Elder RT, Yamamoto A, Cao J
JournalVirology
PubMed ID9657945
'Expression of HIV-1 Vpr causes cell cycle G2 arrest, change in cell shape, and cell death over a large evolutionary distance ranging from human to yeast cells. As a step toward understanding these highly conserved Vpr functions, we have examined the effect of Vpr on cytoskeletal elements and the viability ... More
Viability of Saccharomyces cerevisiae cells exposed to low-amperage electrolysis as assessed by staining procedure and ATP content.
AuthorsGuillou S, Besnard V, El Murr N, Federighi M
JournalInt J Food Microbiol
PubMed ID14527789
'Assessment of yeast viability by plate counts, ATP determination and FUN-1 viability staining was performed to study sublethal injury of Saccharomyces cerevisiae cells submitted to low-amperage electrolysis. Lethal effects of electrolysis were confirmed by all methods, demonstrated by the decrease in viable counts observed during electrolysis. FUN-1 viability staining and ... More
Attenuation of itraconazole fungicidal activity following preexposure of Aspergillus fumigatus to fluconazole.
AuthorsLiu W, Lionakis MS, Lewis RE, Wiederhold N, May GS, Kontoyiannis DP
JournalAntimicrob Agents Chemother
PubMed ID14576123
'Fluconazole (FLC), a triazole with limited activity against Aspergillus species, is frequently used as prophylaxis in leukemia patients and bone marrow transplant recipients. Prior FLC use has been associated with an increasing incidence of invasive aspergillosis in these patients. We hypothesized that prior exposure of Aspergillus fumigatus to FLC could ... More
The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function.
AuthorsMadrid AS, Mancuso J, Cande WZ, Weis K
JournalJ Cell Biol
PubMed ID16682526
'The nuclear pore complex (NPC) is a large channel that spans the two lipid bilayers of the nuclear envelope and mediates transport events between the cytoplasm and the nucleus. Only a few NPC components are transmembrane proteins, and the role of these proteins in NPC function and assembly remains poorly ... More
Flow cytometric assessment of cell structural and functional changes induced by acetic acid in the yeasts Zygosaccharomyces bailii and Saccharomyces cerevisiae.
AuthorsPrudêncio C, Sansonetty F, Côrte-Real M
JournalCytometry
PubMed ID9551607
'Flow cytometry (FCM) was used with different viability dyes to assess changes in cell structure and function induced by acetic acid (AA) in populations of Zygosaccharomyces bailii (AA resistant) and Saccharomyces cerevisiae (AA sensitive). Kinetic changes in esterase activity, intracellular dye processing, and membrane integrity were monitored, and to detect ... More
Enhancement of streptolysin O activity and intrinsic cytotoxic effects of the group A streptococcal toxin, NAD-glycohydrolase.
AuthorsMichos A, Gryllos I, Håkansson A, Srivastava A, Kokkotou E, Wessels MR
JournalJ Biol Chem
PubMed ID16431917
'Streptolysin O (SLO) is a cholesterol-dependent cytolysin produced by the important human pathogen, group A Streptococcus (Streptococcus pyogenes or GAS). In addition to its cytolytic activity, SLO mediates the translocation of GAS NAD-glycohydrolase (NADase) into human epithelial cells in vitro. Production of both NADase and SLO is associated with augmented ... More
Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols.
AuthorsMukherjee PK, Chandra J, Kuhn DM, Ghannoum MA
JournalInfect Immun
PubMed ID12874310
'Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic Candida strains lacking one or more of the drug efflux pumps Cdr1p, Cdr2p, and Mdr1p to determine their role in FLU resistance ... More
Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells.
AuthorsHenry-Stanley MJ, Garni RM, Wells CL,
JournalJ Microbiol Methods
PubMed ID15369865
'The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and ... More
Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton.
AuthorsGoodson HV, Anderson BL, Warrick HM, Pon LA, Spudich JA
JournalJ Cell Biol
PubMed ID8682864
'The organization of the actin cytoskeleton plays a critical role in cell physiology in motile and nonmotile organisms. Nonetheless, the function of the actin based motor molecules, members of the myosin superfamily, is not well understood. Deletion of MYO3, a yeast gene encoding a "classic" myosin I, has no detectable ... More
Fungicidal effect of tyrothricin on Candida albicans.
AuthorsKretschmar M, Nichterlein T, Nebe CT, Hof H, Burger KJ
JournalMycoses
PubMed ID8786757
'Tyrothricin, a polypeptide antibiotic, is active against yeast cells. Tyrothricin was rapidly fungicidal towards Candida albicans. Concentration of four times the minimum inhibitory (25 mg l-1) reduced the yeast numbers by more than 3 log10 within 1 h. Similar results were obtained in a flow cytometric antifungal activity assay using ... More
Rapid susceptibility testing of fungi by flow cytometry using vital staining.
AuthorsWenisch C, Linnau KF, Parschalk B, Zedtwitz-Liebenstein K, Georgopoulos A
JournalJ Clin Microbiol
PubMed ID8968873
'A 1-h assay for antifungal susceptibility testing measuring the impairment of fungal metabolic activity was developed. Yeast viability was analyzed by flow cytometry with a novel fluorescent probe, FUN-1, which emits a red fluorescence when the yeast is metabolically active. For nine Candida albicans strains tested, this method yielded results ... More
Antifungal activity of local anesthetics against Candida species.
AuthorsPina-Vaz C, Rodrigues AG, Sansonetty F, Martinez-De-Oliveira J, Fonseca AF, Mårdh PA
JournalInfect Dis Obstet Gynecol
PubMed ID10968594
'OBJECTIVE: To evaluate the activity of benzydamine, lidocaine, and bupivacaine, three drugs with local anesthetic activity, against Candida albicans and non-albicans strains and to clarify their mechanism of activity. METHODS: The minimal inhibitory concentration (MIC) was determined for 20 Candida strains (18 clinical isolates and two American Type Culture Collection ... More
Determination of cell viability in single or mixed samples using capillary electrophoresis laser-induced fluorescence microfluidic systems.
AuthorsArmstrong DW, He L
JournalAnal Chem
PubMed ID11605830
The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that currently is found for chemical systems. ... More
A plant defense response effector induces microbial apoptosis.
AuthorsNarasimhan ML, Damsz B, Coca MA, Ibeas JI, Yun DJ, Pardo JM, Hasegawa PM, Bressan RA
JournalMol Cell
PubMed ID11684026
Osmotin is a tobacco PR-5 protein that has antifungal activity and is implicated in host-plant defense. We show here that osmotin induces apoptosis in Saccharomyces cerevisiae. Induction of apoptosis was correlated with intracellular accumulation of reactive oxygen species and was mediated by RAS2, but not RAS1. Osmotin treatment resulted in ... More
Viability of endolithic micro-organisms in rocks from the McMurdo Dry Valleys of Antarctica established by confocal and fluorescence microscopy.
AuthorsWierzchos J, De Los Ríos A, Sancho LG, Ascaso C,
JournalJ Microsc
PubMed ID15369484
The rocks of the McMurdo Dry Valleys desert in Antarctica harbour endolithic communities of micro-organisms such as lichens, fungi, cyanobacteria and bacteria. Establishing the physiological status and viability of these microbial colonies in their natural microhabitat has far-reaching implications for understanding the microbial ecology of the harsh environment of this ... More
Human CD4(+)CD25(+) regulatory, contact-dependent T cells induce interleukin 10-producing, contact-independent type 1-like regulatory T cells [corrected].
AuthorsDieckmann D, Bruett CH, Ploettner H, Lutz MB, Schuler G
JournalJ Exp Med
PubMed ID12119349
It has been recently demonstrated that regulatory CD4(+)CD25(+) CD45RO(+) T cells are present in the peripheral blood of healthy adults and exert regulatory function similar to their rodent counterparts. It remains difficult to understand how the small fraction of these T cells that regulate via direct cell-to-cell contact and not ... More
Changes in Candida albicans colonization and morphology under influence of voriconazole.
AuthorsBernhardt H, Knoke M, Bernhardt J
JournalMycoses
PubMed ID14622384
The aim was the investigation of fungal colonization and morphological alterations under the influence of voriconazole in an in vitro system. Voriconazole stopped growth and colonization of Candida albicans (wild type SC5314) on cover slips in microtiter plates dependent on drug concentration, the time of Candida growth before the input ... More
Transcriptional profiling of ubp10 null mutant reveals altered subtelomeric gene expression and insurgence of oxidative stress response.
AuthorsOrlandi I, Bettiga M, Alberghina L, Vai M
JournalJ Biol Chem
PubMed ID14623890
UBP10 codes for a deubiquitinating enzyme of Saccharomyces cerevisiae whose loss of function determines slow growth rate and partial impairment of silencing at telomeres and HM loci. A genome-wide analysis performed on a ubp10 disruptant revealed alterations in expression of subtelomeric genes together with a broad change in the whole ... More
The Ras/protein kinase A pathway acts in parallel with the Mob2/Cbk1 pathway to effect cell cycle progression and proper bud site selection.
AuthorsSchneper L, Krauss A, Miyamoto R, Fang S, Broach JR
JournalEukaryot Cell
PubMed ID14871942
In Saccharomyces cerevisiae, Ras proteins connect nutrient availability to cell growth through regulation of protein kinase A (PKA) activity. Ras proteins also have PKA-independent functions in mitosis and actin repolarization. We have found that mutations in MOB2 or CBK1 confer a slow-growth phenotype in a ras2Delta background. The slow-growth phenotype ... More
Dectin-1 mediates macrophage recognition of Candida albicans yeast but not filaments.
AuthorsGantner BN, Simmons RM, Underhill DM
JournalEMBO J
PubMed ID15729357
The ability of Candida albicans to rapidly and reversibly switch between yeast and filamentous morphologies is crucial to pathogenicity, and it is thought that the filamentous morphology provides some advantage during interaction with the mammalian immune system. Dectin-1 is a receptor that binds beta-glucans and is important for macrophage phagocytosis ... More
Conidial viability assay for rapid susceptibility testing of Aspergillus species.
AuthorsBalajee SA, Marr KA
JournalJ Clin Microbiol
PubMed ID12149322
Antifungal susceptibility testing of filamentous fungi has become more important given the recognition of drug-resistant organisms and the availability of therapies other than amphotericin B (AMB). As current microdilution and E-test methods are limited by a 2 to 3 day incubation time required to obtain results, a more rapid method ... More
Antifungal susceptibility testing of fluconazole by flow cytometry correlates with clinical outcome.
AuthorsWenisch C, Moore CB, Krause R, Presterl E, Pichna P, Denning DW
JournalJ Clin Microbiol
PubMed ID11427554
Susceptibility testing of fungi by flow cytometry (also called fluorescence-activated cell sorting [FACS]) using vital staining with FUN-1 showed a good correlation with the standard M27-A procedure for assessing MICs. In this study we determined MICs for blood culture isolates from patients with candidemia by NCCLS M27-A and FACS methods ... More
Cell cycle-dependent nuclear localization of yeast RNase III is required for efficient cell division.
AuthorsCatala M, Lamontagne B, Larose S, Ghazal G, Elela SA
JournalMol Biol Cell
PubMed ID15090619
Members of the double-stranded RNA-specific ribonuclease III (RNase III) family were shown to affect cell division and chromosome segregation, presumably through an RNA interference-dependent mechanism. Here, we show that in Saccharomyces cerevisiae, where the RNA interference machinery is not conserved, an orthologue of RNase III (Rnt1p) is required for progression ... More
Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast.
AuthorsCope MJ, Yang S, Shang C, Drubin DG
JournalJ Cell Biol
PubMed ID10087264
Ark1p (actin regulating kinase 1) was identified as a yeast protein that binds to Sla2p, an evolutionarily conserved cortical actin cytoskeleton protein. Ark1p and a second yeast protein, Prk1p, contain NH2-terminal kinase domains that are 70% identical. Together with six other putative kinases from a number of organisms, these proteins ... More
Human T cell activation by costimulatory signal-deficient allogeneic cells induces inducible costimulator-expressing anergic T cells with regulatory cell activity.
AuthorsVermeiren J, Ceuppens JL, Van Ghelue M, Witters P, Bullens D, Mages HW, Kroczek RA, Van Gool SW
JournalJ Immunol
PubMed ID15100277
Although immunoregulation by several types of regulatory T cells is now clearly established in mice, the demonstration of such regulatory T cells in humans has been proven more difficult. In this study we demonstrate the induction of anergic regulatory T cells during an MLR performed in the presence of blocking ... More
Mitochondrial fission proteins regulate programmed cell death in yeast.
AuthorsFannjiang Y, Cheng WC, Lee SJ, Qi B, Pevsner J, McCaffery JM, Hill RB, Basañez G, Hardwick JM
JournalGenes Dev
PubMed ID15520274
The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast encode a homolog of human Drp1, a mitochondrial fission protein that was shown previously to promote mammalian cell death and the excessive ... More
Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications.
AuthorsZipper H, Brunner H, Bernhagen J, Vitzthum F
JournalNucleic Acids Res
PubMed ID15249599
The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure-property relationships that affect methods applying SG. These studies ... More
Quantitative variation of biofilms among strains in natural populations of Candida albicans.
AuthorsLi X, Yan Z, Xu J
JournalMicrobiology
PubMed ID12624197
This study examined the quantitative variation of biofilm formation and its relationship to multilocus genotypes in 115 strains of the human pathogenic fungus Candida albicans. These strains were isolated from three sources: 47 from oral cavities of healthy volunteers, 31 from the environment and 37 from the vaginas of patients ... More
c-MIR, a human E3 ubiquitin ligase, is a functional homolog of herpesvirus proteins MIR1 and MIR2 and has similar activity.
AuthorsGoto E, Ishido S, Sato Y, Ohgimoto S, Ohgimoto K, Nagano-Fujii M, Hotta H
JournalJ Biol Chem
PubMed ID12582153
Kaposi's sarcoma associated-herpes virus encodes two proteins, MIR (modulator of immune recognition) 1 and 2, which are involved in the evasion of host immunity. MIR1 and 2 have been shown to function as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1) ... More
Antifungal activity of ibuprofen alone and in combination with fluconazole against Candida species.
AuthorsPina-Vaz C, Sansonetty F, Rodrigues AG, Martinez-De-Oliveira J, Fonseca AF, Mårdh PA
JournalJ Med Microbiol
PubMed ID10966233
Ibuprofen, a non-steroidal anti-inflammatory drug, exhibited antimicrobial activity against Candida albicans and non-albicans strains. At 10 mg/ml, ibuprofen showed a rapid cidal activity against exponential growth phase C. albicans, accompanied by rapid and extensive leakage of intracellular K+, permeation to propidium iodide, lysis of spheroplasts and severe membrane ultrastructural alterations. ... More
Saccharomyces cerevisiae samples stained with FUN-1 dye can be stored at -20 degrees C for later observation.
AuthorsEggleston MD, Marshall PA
JournalJ Microsc
PubMed ID17286700
FUN-1, a fluorescent vital dye, has been observed to form cylindrical intravacuolar structures within the vacuoles of metabolically active yeast cells. FUN-1 staining, which begins as a diffuse pool of fluorescent cytoplasmic stain, uses an unknown endogenous biochemical processing mechanism to compact and form orange-red cylindrical intravacuolar structures within the ... More
Mature dendritic cells derived from human monocytes within 48 hours: a novel strategy for dendritic cell differentiation from blood precursors.
AuthorsDauer M, Obermaier B, Herten J, Haerle C, Pohl K, Rothenfusser S, Schnurr M, Endres S, Eigler A
JournalJ Immunol
PubMed ID12682236
It is widely believed that generation of mature dendritic cells (DCs) with full T cell stimulatory capacity from human monocytes in vitro requires 5-7 days of differentiation with GM-CSF and IL-4, followed by 2-3 days of activation. Here, we report a new strategy for differentiation and maturation of monocyte-derived DCs ... More
Antifungal activity of amiodarone is mediated by disruption of calcium homeostasis.
AuthorsGupta SS, Ton VK, Beaudry V, Rulli S, Cunningham K, Rao R
JournalJ Biol Chem
PubMed ID12754197
The antiarrhythmic drug amiodarone was recently demonstrated to have novel broad range fungicidal activity. We provide evidence that amiodarone toxicity is mediated by disruption of Ca2+ homeostasis in Saccharomyces cerevisiae. In mutants lacking calcineurin and various Ca2+ transporters, including pumps (Pmr1 and Pmc1), channels (Cch1/Mid1 and Yvc1), and exchangers (Vcx1), ... More
Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeasts.
AuthorsMillard PJ, Roth BL, Thi HP, Yue ST, Haugland RP
JournalAppl Environ Microbiol
PubMed ID9212436
A new family of fluorescent probes has been developed for assessing the viability and metabolic activity of yeasts. This class of halogenated unsymmetric cyanine dyes is exemplified by the FUN-1 [2-chloro-4-(2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)- methylidene)-1-phenylquinolinium iodide] stain, a membrane-permeant nucleic acid-binding dye that has been found to give rise to cylindrical intravacuolar structures ... More
Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces.
AuthorsKuhn DM, Chandra J, Mukherjee PK, Ghannoum MA
JournalInfect Immun
PubMed ID11796623
Little is known about fungal biofilms, which may cause infection and antibiotic resistance. In this study, biofilm formation by different Candida species, particularly Candida albicans and C. parapsilosis, was evaluated by using a clinically relevant model of Candida biofilm on medical devices. Candida biofilms were allowed to form on silicone ... More
CO2 enhances the formation, nutrient scavenging and drug resistance properties of C. albicans biofilms.
Authors
JournalNPJ Biofilms Microbiomes
PubMed ID34385462
Transformation of Fonsecaea pedrosoi into sclerotic cells links to the refractoriness of experimental chromoblastomycosis in BALB/c mice via a mechanism involving a chitin-induced impairment of IFN-γ production.
Authors
JournalPLoS Negl Trop Dis
PubMed ID29481557
Possible Role for Allelic Variation in Yeast MED15 in Ecological Adaptation.
Authors
JournalFront Microbiol
PubMed ID34733258
Muriform Cells Can Reproduce by Dividing in an Athymic Murine Model of Chromoblastomycosis due to Fonsecaea pedrosoi.
Authors
JournalAm J Trop Med Hyg
PubMed ID32524944
GP IIb/IIIa-Mediated Platelet Activation and Its Modulation of the Immune Response of Monocytes Against Candida albicans.
Authors
JournalFront Cell Infect Microbiol
PubMed ID34938671
Cytological and genetic consequences for the progeny of a mitotic catastrophe provoked by Topoisomerase II deficiency.
Authors
JournalAging (Albany NY)
PubMed ID31812950
Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae.