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Citas y referencias (47)
Invitrogen™
FUN™ 1 Cell Stain
FUN™ 1 es sonda de viabilidad fluorescente de dos colores para levaduras y hongos. La tinción FUN™ 1 se difundeMás información
| Número de catálogo | Cantidad |
|---|---|
| F7030 | 100 μl |
Número de catálogo F7030
Precio (MXN)
-
Cantidad:
100 μl
FUN™ 1 es sonda de viabilidad fluorescente de dos colores para levaduras y hongos. La tinción FUN™ 1 se difunde pasivamente en una variedad de tipos de células e inicialmente tiñe el citoplasma con fluorescencia verde distribuida difusamente En varias especies comunes de levaduras y hongos, el procesamiento posterior del tinte por células vivas da lugar a la formación de estructuras vacuolares distintas con forma compacta que muestran una llamativa fluorescencia roja, acompañada de la reducción de la fluorescencia citoplasmática verde La formación de estructuras intravacuolares requiere tanto la integridad de la membrana plasmática como la capacidad metabólica Las células muertas tienen una fluorescencia amarillo-verde brillante, sin estructuras rojas discernibles.
Obtenga más información sobre los indicadores de iones, incluidos el calcio, el potasio, el pH y los indicadores de potencial de membrana ›
Obtenga más información sobre los indicadores de iones, incluidos el calcio, el potasio, el pH y los indicadores de potencial de membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCélulas fúngicas, células de levadura
Concentración10 mm
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormatoTubos, portaobjetos
Cantidad100 μl
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo
Línea de productosFUN
Tipo de productoTinción
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Preguntas frecuentes
Can FUN 1 stained cells be examined by flow cytometry?
Live, metabolically active fungi transport FUN 1 into vacuoles to give a red-shifted fluorescence versus green/yellow fluorescence in the nucleus and cytoplasm of dead or metabolically-inactive cells. Is this a reliable indicator of fungal viability?
Citations & References (47)
Citations & References
Abstract
SOD2 functions downstream of Sch9 to extend longevity in yeast.
Journal:Genetics
PubMed ID:12586694
'Signal transduction pathways inactivated during periods of starvation are implicated in the regulation of longevity in organisms ranging from yeast to mammals, but the mechanisms responsible for life-span extension are poorly understood. Chronological life-span extension in S. cerevisiae cyr1 and sch9 mutants is mediated by the stress-resistance proteins Msn2/Msn4 and
Early events in macrophage killing of Aspergillus fumigatus conidia: new flow cytometric viability assay.
Journal:Clin Diagn Lab Immunol
PubMed ID:11687470
'Detailed investigations of macrophage phagocytosis and killing of Aspergillus fumigatus conidia have been limited by technical difficulties in quantifying fungal uptake and viability. In order to study early events in cell pathogen ingestion and killing, we developed a new flow cytometry assay that utilizes the fungus-specific viability dye FUN-1. Metabolically
Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation.
Journal:J Immunol
PubMed ID:11751952
'Immature dendritic cells (DC) reside in peripheral tissues, where they pick up and process incoming pathogens via scavenger receptors or FcR such as FcgammaR and FcepsilonR. At mucosal surfaces, IgA is the main Ig to protect the body from incoming pathogens. In addition, DC are present in high numbers at
In vitro growth and analysis of Candida biofilms.
Journal:Nat Protoc
PubMed ID:19180075
'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion
Viruses activate a genetically conserved cell death pathway in a unicellular organism.
Journal:J Cell Biol
PubMed ID:16061692
'Given the importance of apoptosis in the pathogenesis of virus infections in mammals, we investigated the possibility that unicellular organisms also respond to viral pathogens by activating programmed cell death. The M1 and M2 killer viruses of Saccharomyces cerevisiae encode pore-forming toxins that were assumed to kill uninfected yeast cells