'We describe a capillary electrophoresis (CE) assay to detect G protein-coupled receptor (GPCR)-stimulated G protein GTPase activity in cell membranes expressing alpha2A adrenoreceptor-Galphao1 wild-type (wt) or C351I mutant fusion proteins using a fluorescent, hydrolyzable GTP analogue. As no change in total fluorescence is observed by conversion of substrate to product, ... More
Role and timing of GTP binding and hydrolysis during EF-G-dependent tRNA translocation on the ribosome.
AuthorsWilden B, Savelsbergh A, Rodnina MV, Wintermeyer W
JournalProc Natl Acad Sci U S A
PubMed ID16940356
The translocation of tRNA and mRNA through the ribosome is promoted by elongation factor G (EF-G), a GTPase that hydrolyzes GTP during the reaction. Recently, it was reported that, in contrast to previous observations, the affinity of EF-G was much weaker for GTP than for GDP and that ribosome-catalyzed GDP-GTP ... More
Probing the Ligand-Binding Pocket of BTN3A1.
Authors
JournalJ Med Chem
PubMed ID31268699
Protocol for structural and biochemical analyses of RhoA GTPase.
Authors
JournalSTAR Protoc
PubMed ID34036285
The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.
Authors
JournalElife
PubMed ID29489452
Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding.
Authors
JournalJ Biol Chem
PubMed ID28630045
ISRIB Blunts the Integrated Stress Response by Allosterically Antagonising the Inhibitory Effect of Phosphorylated eIF2 on eIF2B.
Authors
JournalMol Cell
PubMed ID33220178
GTP binding to translation factor eIF2B stimulates its guanine nucleotide exchange activity.
Authors
JournaliScience
PubMed ID34877508
Flow cytometry for real-time measurement of guanine nucleotide binding and exchange by Ras-like GTPases.
Authors
JournalAnal Biochem
PubMed ID18638444
Calcium-induced structural rearrangements release autoinhibition in the Rap-GEF CalDAG-GEFI.
Authors
JournalJ Biol Chem
PubMed ID29622678
Structure of an inactive conformation of GTP-bound RhoA GTPase.