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Invitrogen
Complement C3a Monoclonal Antibody (K13/16-5.7)
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FIGURE: 1 / 6
Complement C3a Antibody (GAU 013-16-02) in WB
Western Blot was performed using Anti-Complement C3a Monoclonal Antibody (K13/16-5.7) (Product # GAU 013-16-02) and a 187 kDa band corresponding to Complement C3 was observed in HepG2 comparison to HEK-293 which is reported to be negative. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Protein transport inhibitor(1X PTI for 4hr) (Lane 2), Hep G2 treated with LPS (10 ng/mL for 18 hr) followed by PTI(1X for 4hr) (Lane 3), HEK-293 (Lane 4), HEK-293 treated with PTI (1X for 4hr) (Lane 5) were electrophoresed ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
GAU 013-16-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
K13/16-5.7
Immunogen
Human C3a.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.07 mg/mL
Amount
428 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
GAU 013-16-02 detects Complement Componet C3a/C3a (desArg) in human samples. This antibody does not cross-react with C4a or C5a.
GAU 013-16-02 has been successfully used in ELISA and Western blot procedures. GAU 013-16-02 can be used as a capture antibody in sandwich ELISA with GAU 017-01-02 as detection antibody.
This antibody detects an epitope that is present on human C3, C3a, and C3a (desArg), but does not cross react with C4a or C5a.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; C3r1; complement component 3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; prepro-C3; unnamed protein product
Gene Aliases: AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1; HEL-S-62p
UniProt ID: (Human) P01024
Entrez Gene ID: (Human) 718
complement activation, lectin pathway
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
complement receptor mediated signaling pathway
proteolysis
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
opsonization
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
neuron remodeling
regulation of complement activation
regulation of complement activation, alternative pathway
leukocyte chemotaxis
B cell activation
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of receptor-mediated endocytosis
positive regulation of phagocytosis
positive regulation of immune response
response to other organism
positive regulation of phagocytosis, engulfment
beta-amyloid clearance
complement-mediated synapse pruning
vertebrate eye-specific patterning
complement activation, GZMK pathway
positive regulation of opsonization
positive regulation of apoptotic cell clearance
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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