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Abnova
RUNX2 Monoclonal Antibody (4E5)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
H00000860-M16A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgM, kappa
Class
Monoclonal
Type
Antibody
Clone
4E5
Immunogen
RUNX2 (NP_001019801.1, 311 a.a. approximately 450 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
See Label
Storage buffer
ascites
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: TSPSIHSTTP LSSTRGTGLP AITDVPRRIS DDDTATSDFC LWPSTLSKKS QAGASELGPF SDPRQFPSIS SLTESRFSNP RMHYPATFTY TPPVTSGMSL GMSATTHYHT YLPPPYPGSS QSQSGPFQTS STPYLYYGTS
Target Information
RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acute myeloid leukemia 3 protein; AKV core binding factor; AML-3; core binding factor alpha 1; core-binding factor, runt domain, alpha subunit 1; nuclear protein; oncogene AML-3; osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2 alpha A; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; transcription factor; transcription factor Cbfa1/Osf2
Gene Aliases: AML3; Cbf; CBF-alpha-1; Cbfa-1; CBFA1; CCD; CCD1; CLCD; LS3; OSF-2; OSF2; PEA2aA; Pebp2a1; PEBP2aA; Pebpa2a
UniProt ID: (Human) Q13950, (Mouse) Q08775
Entrez Gene ID: (Human) 860, (Mouse) 12393
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ATP binding
sequence-specific double-stranded DNA binding
transcription regulatory region sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
protein domain specific binding
chromatin DNA binding
histone deacetylase binding
bHLH transcription factor binding
general transcription initiation factor binding
DNA-binding transcription factor binding
skeletal system development
ossification
osteoblast differentiation
endochondral ossification
osteoblast fate commitment
chondrocyte differentiation
chondrocyte development
osteoblast development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transmembrane receptor protein serine/threonine kinase signaling pathway
cell proliferation
positive regulation of cell proliferation
gene expression
regulation of gene expression
positive regulation of Schwann cell proliferation
positive regulation of gene expression
hemopoiesis
neuron differentiation
T cell differentiation
regulation of ossification
BMP signaling pathway
positive regulation of chondrocyte differentiation
embryonic forelimb morphogenesis
ligamentous ossification
regulation of fibroblast growth factor receptor signaling pathway
odontogenesis of dentin-containing tooth
regulation of odontogenesis of dentin-containing tooth
regulation of cell differentiation
regulation of osteoblast differentiation
positive regulation of osteoblast differentiation
positive regulation of ossification
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell development
cell maturation
embryonic cranial skeleton morphogenesis
skeletal system morphogenesis
stem cell differentiation
positive regulation of multicellular organismal process
SMAD protein signal transduction
response to sodium phosphate
regulation of multicellular organismal development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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