Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

HCS DNA Damage Kit

Catalog number: H10292

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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The HCS DNA Damage kit simultaneously and quantitatively measures two important cell-health parameters: DNA damage and cytotoxicity. DNA damage is detected using an antibody against phosphorylated H2AX (Ser139) which is induced in response to double-strand break (DSB) formation. To detect cytotoxicity, the Image-iT® DEAD™ Green is an impermeant dye in healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.
For Research Use Only. Not for use in diagnostic procedures.


Color: Orange, Green, Blue
Format: 96-well plate
Label or Dye: Alexa Fluor™ 555, Hoechst 33258
Product Size: 1 kit
Emission Class: Visible, UV
Detection Method: Fluorescent
Excitation Class: Visible, UV
For Use With (Equipment): High Content Instrument
Shipping Condition: Dry Ice

Contents & storage

The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
Store at ≤-20°C, desiccate and protect from light.


Manuals & protocols