Development of a high-content screening method for chemicals modulating DNA damage response.
AuthorsKim S, Jun DH, Kim HJ, Jeong KC, Lee CH,
JournalJ Biomol Screen
PubMed ID21233308
The cellular response to DNA damage is emerging as a promising target for cancer therapy. In the present study, the authors exploited the relationship between the level of the phosphorylated form of histone H2AX (?H2AX) and the extent of DNA damage and developed a quantitative, cell-based, high-content screening system for ... More
Induction of glutathione-dependent DNA double-strand breaks by the novel anticancer drug brostallicin.
AuthorsGuirouilh-Barbat J, Zhang YW, Pommier Y,
JournalMol Cancer Ther
PubMed ID19584235
Brostallicin is a DNA minor groove binder in phase II clinical trials. Here, we show that brostallicin induces gamma-H2AX nuclear foci that colocalize with 53BP1 and are dependent on glutathione, as shown by inhibition of those gamma-H2AX foci by l-buthionine sulfoximine. To differentiate brostallicin from the clinically approved minor groove ... More
Free radical scavenging, a-glucosidase inhibitory and lipase inhibitory activities of eighteen Sudanese medicinal plants.
AuthorsElbashir SMI, Devkota HP, Wada M, Kishimoto N, Moriuchi M, Shuto T, Misumi S, Kai H, Watanabe T,
JournalBMC Complement Altern Med
PubMed ID30340582
'Lifestyle-related diseases such as diabetes are steadily increasing worldwide. In Sudan, there are a variety of plant species used traditionally for the treatment of diabetes, obesity and other symptoms which need to be validated through scientific studies for their claimed traditional uses. Therefore, in the current study, the free radical ... More
AuthorsZhou S, Hokugo A, McClendon M, Zhang Z, Bakshi R, Wang L, Segovia LA, Rezzadeh K, Stupp SI, Jarrahy R
JournalBurns
PubMed ID31043333
'Burns are physically debilitating and potentially fatal injuries. The standard-of-care for burn wounds is the coverage with gauze dressings designed to minimize trauma to the regenerating epidermis and dermis during dressing changes. However, deep partial- and full-thickness burns always heal slowly when standard wound care alone is performed. We have ... More
A novel hydroxyphenyl hydrazone derivate YCL0426 inhibits cancer cell proliferation through sequestering iron.
AuthorsLi F, Long L, Xiao J, Wang C, Li W, Li S, Zhao C, Wang L
JournalAnticancer Drugs
PubMed ID28926421
'Cancer cells have an increased requirement for iron than normal cells, and iron chelators are under active consideration for cancer treatment. The metal-sequestering potential and antiproliferative mechanisms of a novel hydroxyphenyl hydrazone derivate YCL0426 were investigated here. Antiproliferative activity of YCL0426 was detected by MTT assay. The iron-sequestering potential was ... More
Combination of Near Infrared Light-Activated Photodynamic Therapy Mediated by Indocyanine Green with Etoposide to Treat Non-Small-Cell Lung Cancer.
AuthorsLuo T, Zhang Q, Lu QB
JournalCancers (Basel)
PubMed ID28587258
'Indocyanine green (ICG) has been reported as a potential near-infrared (NIR) photosensitizer for photodynamic therapy (PDT) of cancer. However the application of ICG-mediated PDT is both intrinsically and physiologically limited. Here we report a combination of ICG-PDT with a chemotherapy drug etoposide (VP-16), aiming to enhance the anticancer efficacy, to ... More
High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro.
AuthorsQu D, Gu Y, Feng L, Han J
JournalFood Chem
PubMed ID28530558
'Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and ... More
Uptake and Toxicity of Respirable Carbon-Rich Uranium-Bearing Particles: Insights into the Role of Particulates in Uranium Toxicity.
Authors
JournalEnviron Sci Technol
PubMed ID34235927
Radiodynamic Therapy Using TAT Peptide-Targeted Verteporfin-Encapsulated PLGA Nanoparticles.
Authors
JournalInt J Mol Sci
PubMed ID34204001
On-tissue polysulfide visualization by surface-enhanced Raman spectroscopy benefits patients with ovarian cancer to predict post-operative chemosensitivity.
Authors
JournalRedox Biol
PubMed ID33752108
KEAP1 Is Required for Artesunate Anticancer Activity in Non-Small-Cell Lung Cancer.
Authors
JournalCancers (Basel)
PubMed ID33920029
Procaspase-3-activating compound 1 stabilizes hypoxia-inducible factor 1a and induces DNA damage by sequestering ferrous iron.
AuthorsLi F, Wei A, Bu L, Long L, Chen W, Wang C, Zhao C, Wang L
JournalCell Death Dis
PubMed ID30287840
Procaspase-3-activating compound 1 (PAC-1) induces procaspase-3 activation via zinc chelation. However, whether PAC-1 employs other mechanisms remains unknown. Here we systematically screened for potent PAC-1 targets using 29 enhanced green fluorescent protein-labeled reporter cell lines and identified hypoxia-inducible factor 1a (HIF1a) and RAD51 pathways as PAC-1 targets. These results were ... More
Trehalose to cryopreserve human pluripotent stem cells.
AuthorsNtai A, La Spada A, De Blasio P, Biunno I
JournalStem Cell Res
PubMed ID30071393
The successful exploitation of human pluripotent stem cells (hPSCs) for research, translational or commercial reasons requires the implementation of a simple and efficient cryopreservation method. Cryopreservation is usually performed with dimethylsulphoxide (DMSO), in addition to animal proteins. However, even at sub-toxic levels, DMSO diminishes the pluripotency capacity of hPSCs and ... More
Simultaneous delivery of olaparib and carboplatin in PEGylated liposomes imparts this drug combination hypersensitivity and selectivity for breast tumor cells.
AuthorsNovohradsky V, Zajac J, Vrana O, Kasparkova J, Brabec V,
JournalOncotarget
PubMed ID29983873
Combination regiments involving platinum anticancer drugs and agents with unrelated mechanisms of action are a subject of widespread interest. Here, we show that synergistic toxic action in cancer cells of combinations of antitumor platinum drug carboplatin and effective PARP inhibitor olaparib is considerably improved if these combined drugs are encapsulated ... More
Apigenin and hesperidin augment the toxic effect of doxorubicin against HepG2 cells.
AuthorsKorga A, Ostrowska M, Jozefczyk A, Iwan M, Wojcik R, Zgorka G, Herbet M, Vilarrubla GG, Dudka J,
JournalBMC Pharmacol Toxicol
PubMed ID31053173
Hepatocellular carcinoma (HCC) is one of the most common malignancies, with an increasing incidence. Despite the fact that systematic chemotherapy with a doxorubicin provides only marginal improvements in survival of the HCC patients, the doxorubicin is being used in transarterial therapies or combined with the target drug - sorafenib. The ... More