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          Invitrogen

          GC Monoclonal Antibody (5E8), Biotin

          View all (31) GC antibodies

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          Cite GC Monoclonal Antibody (5E8), Biotin

          Additional Information:

          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (1)
          GC Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.
          GC Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          GC Antibody (HYB 249-02B-005) in ELISA

          Sandwich ELISA of total VDBP using HYB 249-05 as the capture antibody and HYB 249-02B as the biotinylated detection antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GC Antibody in ELISA (ELISA)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          HYB 249-02B-005

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          -

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          5E8

          Immunogen

          Gc-globulin isolated from human plasma.

          Conjugate

          Biotin Biotin Biotin

          Form

          Liquid

          Concentration

          1.10 mg/mL

          Amount

          55 µg

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Wet ice

          RRID

          AB_1074570

          Product Specific Information

          HYB 249-02B-005 detects Gc-Globulin in human samples.

          HYB 249-02B-005 is suggested for use in ELISA and Western blotting techniques. Strong reaction is seen in ELISA with Gc-globulin coated directly onto the microtiter well and when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin. In Western blotting after SDS-PAGE, HYB 249-02B-005 reacts with Gc-globulin in both reduced as well as unreduced forms.

          The HYB 249-02B-005 immunogen is Gc-Globulin found in human plasma cells.

          NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

          Target Information

          GC (vitamin D-binding protein) is involved in vitamin D transport/storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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