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Indo-1, AM, cell permeant
Citations & References (1088)
Invitrogen™

Indo-1, AM, cell permeant

Indo-1, AM, cell permeant

Have Questions?
Catalog NumberQuantity
I12031 mg
Catalog number I1203
Price (USD)
251.00
Each
Quantity:
1 mg
Price (USD)
251.00
Each

Indo-1, AM is a high affinity, intracellular calcium indicator (Kd ∼0.23 μM) that is ratiometric and UV light—excitable. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading and is also available in special packaging (I-1223) and in a DMSO solution (I-1226).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeIndo-1
Emission Wavelength401 nm
Excitation Wavelength330 nm
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Application)Calcium Indicator, Flow Cytometry
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product TypeCalcium Indicator
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What indicator dye can I use to measure calcium flux by flow cytometry?

Indo-1, AM is the preferred stain for flow cytometry, where it is more practical to use a single laser for excitation, usually the 351–364 nm spectral lines of the argon-ion laser, and monitor two emissions. The emission maximum of Indo-1 shifts from ~475 nm in Ca2+-free medium to ~400 nm when the dye is saturated with Ca2+. Indo-1, AM is particularly suited for multicolor fluorescence applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (1088)

Citations & References
Abstract
Modulation of myocardial function and [Ca2+] sensitivity by moderate hypothermia in guinea pig isolated hearts.
Authors:Stowe DF,Fujita S,An J,Paulsen RA,Varadarajan SG,Smart SC
Journal:The American journal of physiology
PubMed ID:10600852
FDF03, a novel inhibitory receptor of the immunoglobulin superfamily, is expressed by human dendritic and myeloid cells.
Authors:Fournier N,Chalus L,Durand I,Garcia E,Pin JJ,Churakova T,Patel S,Zlot C,Gorman D,Zurawski S,Abrams J,Bates EE,Garrone P
Journal:Journal of immunology (Baltimore, Md. : 1950)
PubMed ID:10903717
CD5 antibodies increase intracellular ionized calcium concentration in T cells.
Authors:June CH,Rabinovitch PS,Ledbetter JA
Journal:Journal of immunology (Baltimore, Md. : 1950)
PubMed ID:3106489
The binding of a variety of monoclonal antibodies to the CD5 (T, gp67) pan T cell differentiation antigen has been shown to potentiate T cell proliferation. In this paper we show that CD5 monoclonal antibodies cause increased intracellular free calcium concentration ([Ca2+]i) in T cells. An increase in [Ca2+]i occurred ... More
Nitric oxide is an upstream signal of vascular endothelial growth factor-induced extracellular signal-regulated kinase1/2 activation in postcapillary endothelium.
Authors:Parenti A, Morbidelli L, Cui XL, Douglas JG, Hood JD, Granger HJ, Ledda F, Ziche M
Journal:J Biol Chem
PubMed ID:9461619
We recently demonstrated that nitric oxide (NO) significantly contributes to the mitogenic effect of vascular endothelial growth factor (VEGF), suggesting a role for the NO pathway in the signaling cascade following kinase-derivative receptor activation in vascular endothelium. The aim of this study was to investigate the intracellular pathways linked to ... More
Cultured postnatal rat septohippocampal neurons change intracellular calcium in response to ethanol and nerve growth factor.
Authors:Webb B, Suarez SS, Heaton MB, Walker DW
Journal:Brain Res
PubMed ID:9459553
Ethanol exposure affects cellular mechanisms involved in the regulation of calcium (Ca2+) homeostasis. Neurotrophins, such as nerve growth factor (NGF), stabilize intracellular Ca2+([Ca2+]i) during a variety of neurotoxic insults. In this study, changes in [Ca2+]i during treatment with ethanol and NGF were measured at the cell body of neurons using ... More
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