Invitrogen™

Image-iT™ LIVE Green Poly Caspases Detection Kit, for microscopy

Catalog number: I35104
Invitrogen™

Image-iT™ LIVE Green Poly Caspases Detection Kit, for microscopy

Catalog number: I35104
Catalog Number
I35104
Unit Size
1 kit
Price (USD)
285.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
I351041 kit
285.00
Your Price:
***
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The Image-iT™ LIVE Green Poly Caspases Detection Kit employs a novel approach to detect active caspases that is based on a fluorescent inhibitor of caspases (FLICA™) methodology, essentially an affinity label. The reagent associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amine acid sequence. For poly caspases, this recognition sequence is valine-alanine-aspartic acid (VAD). A fluorescein group is attached as a reporter. The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining green-fluorescent signal is a direct measure of the amount of active caspase that was present at the time the inhibitor was added.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)
Fluorescence Microscope
Product Line
Image-iT™
Shipping Condition
Room Temperature
Conjugate
FAM, FAM-VAD-FMK, Propidium Iodide, Hoechst 33342
Format
Slide
Product Type
Caspases Green Detection Kit

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.

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Citations & References